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Title: Inflammatory Bowel Disease Gene Discovery Final Report CRADA No. TC-1335-96

Abstract

The ultimate goal of this project is to identify the human gene( s) responsible for the disorder known as IBD. As a first step towards this goal, the Parties agreed to carry out experiments to c011fim1 and refine linkage of IBD to the chromosomal region( s) of interest identified by gene/Networks, Inc. If results confirmed association of these region(s) with the disease, the Parties planned to complete a physical map of the genetically defined region(s). The work was planned in two phases. The desired products resulting from Phase I were BAC clone(s) containing the genetic marker(s) identified by gene\Networks, Inc. as potentially linked to IBD, plasmid subclones of those BAC(s), and new genetic markers developed from these plasmid subclones. The newly developed markers would be genotyped by gene/Networks, Inc. to ascertain evidence for linkage or non-linkage of IBD to this region. If non-linkage was indicated, the project would move to investigation of other candidate chromosomal regions. Where linkage was indicated, the project would move to Phase II, in which a physical map of the candidate region(s) would be developed. The products of this phase would be contig(s) of BAC clones in the region exhibiting linkage to IBD, as well asmore » plasmid subclones of the BACs and further genetic marker development. There would also be continued genotyping with new polymorphic markers during this phase. It was anticipated that clones identified and developed during these two phases would provide the physical resources for eventual disease gene discovery.« less

Authors:
 [1];  [2]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. gene/Networks, Inc., Alameda, CA (United States)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1430922
Report Number(s):
LLNL-TR-747675
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Olsen, A. S., and Wake, C. T.. Inflammatory Bowel Disease Gene Discovery Final Report CRADA No. TC-1335-96. United States: N. p., 2018. Web. doi:10.2172/1430922.
Olsen, A. S., & Wake, C. T.. Inflammatory Bowel Disease Gene Discovery Final Report CRADA No. TC-1335-96. United States. doi:10.2172/1430922.
Olsen, A. S., and Wake, C. T.. Mon . "Inflammatory Bowel Disease Gene Discovery Final Report CRADA No. TC-1335-96". United States. doi:10.2172/1430922. https://www.osti.gov/servlets/purl/1430922.
@article{osti_1430922,
title = {Inflammatory Bowel Disease Gene Discovery Final Report CRADA No. TC-1335-96},
author = {Olsen, A. S. and Wake, C. T.},
abstractNote = {The ultimate goal of this project is to identify the human gene( s) responsible for the disorder known as IBD. As a first step towards this goal, the Parties agreed to carry out experiments to c011fim1 and refine linkage of IBD to the chromosomal region( s) of interest identified by gene/Networks, Inc. If results confirmed association of these region(s) with the disease, the Parties planned to complete a physical map of the genetically defined region(s). The work was planned in two phases. The desired products resulting from Phase I were BAC clone(s) containing the genetic marker(s) identified by gene\Networks, Inc. as potentially linked to IBD, plasmid subclones of those BAC(s), and new genetic markers developed from these plasmid subclones. The newly developed markers would be genotyped by gene/Networks, Inc. to ascertain evidence for linkage or non-linkage of IBD to this region. If non-linkage was indicated, the project would move to investigation of other candidate chromosomal regions. Where linkage was indicated, the project would move to Phase II, in which a physical map of the candidate region(s) would be developed. The products of this phase would be contig(s) of BAC clones in the region exhibiting linkage to IBD, as well as plasmid subclones of the BACs and further genetic marker development. There would also be continued genotyping with new polymorphic markers during this phase. It was anticipated that clones identified and developed during these two phases would provide the physical resources for eventual disease gene discovery.},
doi = {10.2172/1430922},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Mar 12 00:00:00 EDT 2018},
month = {Mon Mar 12 00:00:00 EDT 2018}
}

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