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Title: Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech ( Fagus sylvatica )

Abstract

Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.

Authors:
 [1];  [2];  [3];  [4];  [4];  [4];  [5];  [4];  [6]
  1. Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.; Laboratory for Environmental and Life Sciences, University of Nova Gorica, Glavni Trg 9 – SI-5261, Vipava, Slovenia.
  2. Laboratory for Environmental and Life Sciences, University of Nova Gorica, Glavni Trg 9 – SI-5261, Vipava, Slovenia.; Dipartimento di Scienze Mediche Chirurgiche e della Salute Trieste, Universita degli Studi di Trieste, Friuli-Venezia Giulia, Italy.
  3. Biotechnical Faculty, Department of Agronomy, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
  4. Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia.
  5. Slovenian Forestry Institute, Večna pot 2, SI-1000 Ljubljana, Slovenia.; Pacific Northwest National Laboratory, Richland, WA 99354, USA.
  6. Laboratory for Environmental and Life Sciences, University of Nova Gorica, Glavni Trg 9 – SI-5261, Vipava, Slovenia.
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1430445
Report Number(s):
PNNL-SA-132844
Journal ID: ISSN 0045-5067
DOE Contract Number:
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Canadian Journal of Forest Research; Journal Volume: 47; Journal Issue: 11
Country of Publication:
United States
Language:
English
Subject:
beach; drought; superoxide dismutase; oxidative stress; glutathione peroxidase

Citation Formats

Popović, Milica, Gregori, Marco, Vodnik, Dominik, Ferlan, Mitja, Mrak, Tanja, Štraus, Ines, McDowell, Nathan G., Kraigher, Hojka, and de Marco, Ario. Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech ( Fagus sylvatica ). United States: N. p., 2017. Web. doi:10.1139/cjfr-2016-0530.
Popović, Milica, Gregori, Marco, Vodnik, Dominik, Ferlan, Mitja, Mrak, Tanja, Štraus, Ines, McDowell, Nathan G., Kraigher, Hojka, & de Marco, Ario. Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech ( Fagus sylvatica ). United States. doi:10.1139/cjfr-2016-0530.
Popović, Milica, Gregori, Marco, Vodnik, Dominik, Ferlan, Mitja, Mrak, Tanja, Štraus, Ines, McDowell, Nathan G., Kraigher, Hojka, and de Marco, Ario. Wed . "Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech ( Fagus sylvatica )". United States. doi:10.1139/cjfr-2016-0530.
@article{osti_1430445,
title = {Identification of stress biomarkers for drought and increased soil temperature in seedlings of European beech ( Fagus sylvatica )},
author = {Popović, Milica and Gregori, Marco and Vodnik, Dominik and Ferlan, Mitja and Mrak, Tanja and Štraus, Ines and McDowell, Nathan G. and Kraigher, Hojka and de Marco, Ario},
abstractNote = {Drought is an environmental stress that impacts plant productivity. Projections show both an increase in intense rain events and a reduction in the number of rain days, conditions that leads to increased risk of drought. Consequently, the identification of molecular biomarkers suitable for evaluating the impact of water deprivation conditions on forest plant seedlings is of significant value for monitoring purposes and forest management. In this study, we evaluated a biochemical methodology for the assessment of drought stress coupled with variable soil temperature in European beech (Fagus sylvatica L.) seedlings by analyzing a set of metabolites and enzymes involved in free radical scavenging and cell wall synthesis. The results indicate that the specific activities and isoform profile of superoxide dismutases and glutathione peroxidases together with the variation of phenolic compounds enable discrimination between seedlings with different degrees of photosynthetic activity. This approach represents a promising platform for the assessment of drought stress in forest trees and could serve for enhancing selection and breeding practices, allowing for plants that are more tolerant of abiotic stress.},
doi = {10.1139/cjfr-2016-0530},
journal = {Canadian Journal of Forest Research},
number = 11,
volume = 47,
place = {United States},
year = {Wed Nov 01 00:00:00 EDT 2017},
month = {Wed Nov 01 00:00:00 EDT 2017}
}