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Title: A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley

Abstract

Starch and fructan are two important carbohydrates in many flowering plants and in human diets. Understanding how plants allocate photosynthates and how they prioritize synthesis of different carbohydrates during development is essential in efforts to improve cereals for increased stress tolerance and for desirable carbohydrate compositions in food and feed. We report the coordinated synthesis of starch and fructan in barley, orchestrated by two functionally opposing transcription factors encoded from two alternative promoters, one intronic/exonic, harbored on a single gene. . This dual-transcription factor system employs an autoregulatory, antagonsitic mechanism in sensing sucrose at one promoter, potentially via sucrose/glucose/fructose/trehalose 6-phosphate signaling, and conduct a coordinated synthesis of starch and fructan synthesis by competitive transcription factor binding to the second promoter The finding of an intron/exon-spanning promoter in a hosting gene, resulting in proteins with distinct functions, contributes to our appreciation of the complexity of the plant genome As a case in point for the physiological role of the antagonistic transcription factor system, we have demonstrated that it can be exploited in breeding barley with tailored amounts of fructan for production of specialty food ingredients.

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1430437
Report Number(s):
PNNL-SA-130518
Journal ID: ISSN 1674-2052; 49714
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Molecular Plant; Journal Volume: 10; Journal Issue: 12
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Jin, Yunkai, Fei, Mingliang, Rosenquist, Sara, Jin, Lu, Gohil, Suresh, Sandström, Corine, Olsson, Helena, Persson, Cecilia, Höglund, Anna-Stina, Fransson, Gunnel, Ruan, Ying, Åman, Per, Jansson, Christer, Liu, Chunlin, Andersson, Roger, and Sun, Chuanxin. A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley. United States: N. p., 2017. Web. doi:10.1016/j.molp.2017.10.013.
Jin, Yunkai, Fei, Mingliang, Rosenquist, Sara, Jin, Lu, Gohil, Suresh, Sandström, Corine, Olsson, Helena, Persson, Cecilia, Höglund, Anna-Stina, Fransson, Gunnel, Ruan, Ying, Åman, Per, Jansson, Christer, Liu, Chunlin, Andersson, Roger, & Sun, Chuanxin. A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley. United States. doi:10.1016/j.molp.2017.10.013.
Jin, Yunkai, Fei, Mingliang, Rosenquist, Sara, Jin, Lu, Gohil, Suresh, Sandström, Corine, Olsson, Helena, Persson, Cecilia, Höglund, Anna-Stina, Fransson, Gunnel, Ruan, Ying, Åman, Per, Jansson, Christer, Liu, Chunlin, Andersson, Roger, and Sun, Chuanxin. Fri . "A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley". United States. doi:10.1016/j.molp.2017.10.013.
@article{osti_1430437,
title = {A Dual-Promoter Gene Orchestrates the Sucrose-Coordinated Synthesis of Starch and Fructan in Barley},
author = {Jin, Yunkai and Fei, Mingliang and Rosenquist, Sara and Jin, Lu and Gohil, Suresh and Sandström, Corine and Olsson, Helena and Persson, Cecilia and Höglund, Anna-Stina and Fransson, Gunnel and Ruan, Ying and Åman, Per and Jansson, Christer and Liu, Chunlin and Andersson, Roger and Sun, Chuanxin},
abstractNote = {Starch and fructan are two important carbohydrates in many flowering plants and in human diets. Understanding how plants allocate photosynthates and how they prioritize synthesis of different carbohydrates during development is essential in efforts to improve cereals for increased stress tolerance and for desirable carbohydrate compositions in food and feed. We report the coordinated synthesis of starch and fructan in barley, orchestrated by two functionally opposing transcription factors encoded from two alternative promoters, one intronic/exonic, harbored on a single gene. . This dual-transcription factor system employs an autoregulatory, antagonsitic mechanism in sensing sucrose at one promoter, potentially via sucrose/glucose/fructose/trehalose 6-phosphate signaling, and conduct a coordinated synthesis of starch and fructan synthesis by competitive transcription factor binding to the second promoter The finding of an intron/exon-spanning promoter in a hosting gene, resulting in proteins with distinct functions, contributes to our appreciation of the complexity of the plant genome As a case in point for the physiological role of the antagonistic transcription factor system, we have demonstrated that it can be exploited in breeding barley with tailored amounts of fructan for production of specialty food ingredients.},
doi = {10.1016/j.molp.2017.10.013},
journal = {Molecular Plant},
number = 12,
volume = 10,
place = {United States},
year = {Fri Dec 01 00:00:00 EST 2017},
month = {Fri Dec 01 00:00:00 EST 2017}
}