skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Climate-simulated raceway pond culturing: quantifying the maximum achievable annual biomass productivity of Chlorella sorokiniana in the contiguous USA

Abstract

Chlorella sorokiniana (DOE 1412) emerged as one of the most promising microalgae strains from the NAABB consortium project, with a remarkable doubling time under optimal conditions of 2.57 hr-1. However, its maximum achievable annual biomass productivity in outdoor ponds in the contiguous United States remained unknown. In order to address this knowledge gap, this alga was cultured in indoor LED-lighted and temperature-controlled raceways in nutrient replete freshwater (BG-11) medium at pH 7 under conditions simulating the daily sunlight intensity and water temperature fluctuations during three seasons in Southern Florida, an optimal outdoor pond culture location for this organism identified by biomass growth modeling. Prior strain characterization indicated that the average maximum specific growth rate (µmax) at 36 ºC declined continuously with pH, with µmax corresponding to 5.92, 5.83, 4.89, and 4.21 day-1 at pH 6, 7, 8, and 9, respectively. In addition, the maximum specific growth rate declined nearly linearly with increasing salinity until no growth was observed above 35 g/L NaCl. In the climate-simulated culturing studies, the volumetric ash-free dry weight-based biomass productivities during the linear growth phase were 57, 69, and 97 mg/L-day for 30-year average light and temperature simulations for January (winter), March (spring), and July (summer),more » respectively, which corresponds to average areal productivities of 11.6, 14.1, and 19.9 g/m2-day at a constant pond depth of 20.5 cm. The photosynthetic efficiencies (PAR) in the three climate-simulated pond culturing experiments ranged from 4.1 to 5.1%. The annual biomass productivity was estimated as ca. 15 g/m2-day, nearly double the U.S. Department of Energy (DOE) 2015 State of Technology annual cultivation productivity of 8.5 g/m2-day, but this is still significantly below the projected 2022 target of ca. 25 g/m2-day (U.S. DOE, 2016) for economic microalgal biofuel production, indicating the need for additional research in strain biology and system engineering.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1430414
Report Number(s):
PNNL-SA-124328
Journal ID: ISSN 0921-8971; BM0108010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of Applied Phycology
Additional Journal Information:
Journal Volume: 30; Journal Issue: 1; Journal ID: ISSN 0921-8971
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
Chlorella sorokiniana; annual biomass productivity; climate-simulation pond culture; maximum specific growth rate as a function of pH; maximum specific growth rate as a function of salinity; photosyn

Citation Formats

Huesemann, M., Chavis, A., Edmundson, S., Rye, D., Hobbs, S., Sun, N., and Wigmosta, M. Climate-simulated raceway pond culturing: quantifying the maximum achievable annual biomass productivity of Chlorella sorokiniana in the contiguous USA. United States: N. p., 2017. Web. doi:10.1007/s10811-017-1256-6.
Huesemann, M., Chavis, A., Edmundson, S., Rye, D., Hobbs, S., Sun, N., & Wigmosta, M. Climate-simulated raceway pond culturing: quantifying the maximum achievable annual biomass productivity of Chlorella sorokiniana in the contiguous USA. United States. doi:10.1007/s10811-017-1256-6.
Huesemann, M., Chavis, A., Edmundson, S., Rye, D., Hobbs, S., Sun, N., and Wigmosta, M. Wed . "Climate-simulated raceway pond culturing: quantifying the maximum achievable annual biomass productivity of Chlorella sorokiniana in the contiguous USA". United States. doi:10.1007/s10811-017-1256-6.
@article{osti_1430414,
title = {Climate-simulated raceway pond culturing: quantifying the maximum achievable annual biomass productivity of Chlorella sorokiniana in the contiguous USA},
author = {Huesemann, M. and Chavis, A. and Edmundson, S. and Rye, D. and Hobbs, S. and Sun, N. and Wigmosta, M.},
abstractNote = {Chlorella sorokiniana (DOE 1412) emerged as one of the most promising microalgae strains from the NAABB consortium project, with a remarkable doubling time under optimal conditions of 2.57 hr-1. However, its maximum achievable annual biomass productivity in outdoor ponds in the contiguous United States remained unknown. In order to address this knowledge gap, this alga was cultured in indoor LED-lighted and temperature-controlled raceways in nutrient replete freshwater (BG-11) medium at pH 7 under conditions simulating the daily sunlight intensity and water temperature fluctuations during three seasons in Southern Florida, an optimal outdoor pond culture location for this organism identified by biomass growth modeling. Prior strain characterization indicated that the average maximum specific growth rate (µmax) at 36 ºC declined continuously with pH, with µmax corresponding to 5.92, 5.83, 4.89, and 4.21 day-1 at pH 6, 7, 8, and 9, respectively. In addition, the maximum specific growth rate declined nearly linearly with increasing salinity until no growth was observed above 35 g/L NaCl. In the climate-simulated culturing studies, the volumetric ash-free dry weight-based biomass productivities during the linear growth phase were 57, 69, and 97 mg/L-day for 30-year average light and temperature simulations for January (winter), March (spring), and July (summer), respectively, which corresponds to average areal productivities of 11.6, 14.1, and 19.9 g/m2-day at a constant pond depth of 20.5 cm. The photosynthetic efficiencies (PAR) in the three climate-simulated pond culturing experiments ranged from 4.1 to 5.1%. The annual biomass productivity was estimated as ca. 15 g/m2-day, nearly double the U.S. Department of Energy (DOE) 2015 State of Technology annual cultivation productivity of 8.5 g/m2-day, but this is still significantly below the projected 2022 target of ca. 25 g/m2-day (U.S. DOE, 2016) for economic microalgal biofuel production, indicating the need for additional research in strain biology and system engineering.},
doi = {10.1007/s10811-017-1256-6},
journal = {Journal of Applied Phycology},
issn = {0921-8971},
number = 1,
volume = 30,
place = {United States},
year = {2017},
month = {9}
}