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Title: A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements

Abstract

To better understand disease conditions and environmental perturbations, multi-omic studies (i.e. proteomic, lipidomic, metabolomic, etc. analyses) are vastly increasing in popularity. In a multi-omic study, a single sample is typically extracted in multiple ways and numerous analyses are performed using different instruments. Thus, one sample becomes many analyses, making high throughput and reproducible evaluations a necessity. One way to address the numerous samples and varying instrumental conditions is to utilize a flow injection analysis (FIA) system for rapid sample injection. While some FIA systems have been created to address these challenges, many have limitations such as high consumable costs, low pressure capabilities, limited pressure monitoring and fixed flow rates. To address these limitations, we created an automated, customizable FIA system capable of operating at diverse flow rates (~50 nL/min to 500 µL/min) to accommodate low- and high-flow instrument sources. This system can also operate at varying analytical throughputs from 24 to 1200 samples per day to enable different MS analysis approaches. Applications ranging from native protein analyses to molecular library construction were performed using the FIA system. The results from these studies showed a highly robust platform, providing consistent performance over many days without carryover as long as washing buffersmore » specific to each molecular analysis were utilized.« less

Authors:
 [1];  [1];  [1];  [1]; ORCiD logo [1];  [1];  [1];  [1];  [1];  [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, United States
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1427915
Report Number(s):
PNNL-SA-128111
Journal ID: ISSN 0003-2700; 400412000
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry; Journal Volume: 90; Journal Issue: 1
Country of Publication:
United States
Language:
English

Citation Formats

Orton, Daniel J., Tfaily, Malak M., Moore, Ronald J., LaMarche, Brian L., Zheng, Xueyun, Fillmore, Thomas L., Chu, Rosalie K., Weitz, Karl K., Monroe, Matthew E., Kelly, Ryan T., Smith, Richard D., and Baker, Erin S. A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements. United States: N. p., 2017. Web. doi:10.1021/acs.analchem.7b02986.
Orton, Daniel J., Tfaily, Malak M., Moore, Ronald J., LaMarche, Brian L., Zheng, Xueyun, Fillmore, Thomas L., Chu, Rosalie K., Weitz, Karl K., Monroe, Matthew E., Kelly, Ryan T., Smith, Richard D., & Baker, Erin S. A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements. United States. doi:10.1021/acs.analchem.7b02986.
Orton, Daniel J., Tfaily, Malak M., Moore, Ronald J., LaMarche, Brian L., Zheng, Xueyun, Fillmore, Thomas L., Chu, Rosalie K., Weitz, Karl K., Monroe, Matthew E., Kelly, Ryan T., Smith, Richard D., and Baker, Erin S. Wed . "A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements". United States. doi:10.1021/acs.analchem.7b02986.
@article{osti_1427915,
title = {A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements},
author = {Orton, Daniel J. and Tfaily, Malak M. and Moore, Ronald J. and LaMarche, Brian L. and Zheng, Xueyun and Fillmore, Thomas L. and Chu, Rosalie K. and Weitz, Karl K. and Monroe, Matthew E. and Kelly, Ryan T. and Smith, Richard D. and Baker, Erin S.},
abstractNote = {To better understand disease conditions and environmental perturbations, multi-omic studies (i.e. proteomic, lipidomic, metabolomic, etc. analyses) are vastly increasing in popularity. In a multi-omic study, a single sample is typically extracted in multiple ways and numerous analyses are performed using different instruments. Thus, one sample becomes many analyses, making high throughput and reproducible evaluations a necessity. One way to address the numerous samples and varying instrumental conditions is to utilize a flow injection analysis (FIA) system for rapid sample injection. While some FIA systems have been created to address these challenges, many have limitations such as high consumable costs, low pressure capabilities, limited pressure monitoring and fixed flow rates. To address these limitations, we created an automated, customizable FIA system capable of operating at diverse flow rates (~50 nL/min to 500 µL/min) to accommodate low- and high-flow instrument sources. This system can also operate at varying analytical throughputs from 24 to 1200 samples per day to enable different MS analysis approaches. Applications ranging from native protein analyses to molecular library construction were performed using the FIA system. The results from these studies showed a highly robust platform, providing consistent performance over many days without carryover as long as washing buffers specific to each molecular analysis were utilized.},
doi = {10.1021/acs.analchem.7b02986},
journal = {Analytical Chemistry},
number = 1,
volume = 90,
place = {United States},
year = {Wed Dec 13 00:00:00 EST 2017},
month = {Wed Dec 13 00:00:00 EST 2017}
}