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Title: Sol-Generating Chemical Vapor into Liquid (SG-CViL) deposition – a facile method for encapsulation of diverse cell types in silica matrices

Abstract

In nature, cells perform a variety of complex functions such as sensing, catalysis, and energy conversion which hold great potential for biotechnological device construction. However, cellular sensitivity to ex vivo environments necessitates development of bio–nano interfaces which allow integration of cells into devices and maintain their desired functionality. In order to develop such an interface, the use of a novel Sol-Generating Chemical Vapor into Liquid (SG-CViL) deposition process for whole cell encapsulation in silica was explored. In SG-CViL, the high vapor pressure of tetramethyl orthosilicate (TMOS) is utilized to deliver silica into an aqueous medium, creating a silica sol. Cells are then mixed with the resulting silica sol, facilitating encapsulation of cells in silica while minimizing cell contact with the cytotoxic products of silica generating reactions (i.e. methanol), and reduce exposure of cells to compressive stresses induced from silica condensation reactions. Using SG-CVIL, Saccharomyces cerevisiae (S. cerevisiae) engineered with an inducible beta galactosidase system were encapsulated in silica solids and remained both viable and responsive 29 days post encapsulation. By tuning SG-CViL parameters, thin layer silica deposition on mammalian HeLa and U87 human cancer cells was also achieved. Thus, the ability to encapsulate various cell types in either a multimore » cell (S. cerevisiae) or a thin layer (HeLa and U87 cells) fashion shows the promise of SG-CViL as an encapsulation strategy for generating cell–silica constructs with diverse functions for incorporation into devices for sensing, bioelectronics, biocatalysis, and biofuel applications.« less

Authors:
 [1];  [2];  [3];  [4]
  1. New Mexico Inst. of Mining and Technology, Socorro, NM (United States). Materials Engineering Dept.
  2. New Mexico Inst. of Mining and Technology, Socorro, NM (United States). Biology Dept.
  3. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Bioenergy and Biodefense Technologies Dept.
  4. New Mexico Inst. of Mining and Technology, Socorro, NM (United States). Materials and Chemical Engineering Dept.
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH); Univ. of Colorado, Boulder, CO (United States)
OSTI Identifier:
1426898
Report Number(s):
SAND2014-17515J
Journal ID: ISSN 2050-750X; JMCBDV; 537380
DOE Contract Number:  
AC04-94AL85000; 151375; 5P20RR016480-12; 8 P20 GM103451-12
Resource Type:
Journal Article
Journal Name:
Journal of Materials Chemistry. B
Additional Journal Information:
Journal Volume: 3; Journal Issue: 6; Journal ID: ISSN 2050-750X
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 36 MATERIALS SCIENCE; Sol-gel, Silica; Living hybrid biomaterials; thin layer deposition; Cellular encapsulation/entrapment; Solgenerating chemical vapor into liquid

Citation Formats

Johnston, Robert, Rogelj, Snezna, Harper, Jason C., and Tartis, Michaelann. Sol-Generating Chemical Vapor into Liquid (SG-CViL) deposition – a facile method for encapsulation of diverse cell types in silica matrices. United States: N. p., 2014. Web. doi:10.1039/C4TB01349B.
Johnston, Robert, Rogelj, Snezna, Harper, Jason C., & Tartis, Michaelann. Sol-Generating Chemical Vapor into Liquid (SG-CViL) deposition – a facile method for encapsulation of diverse cell types in silica matrices. United States. doi:10.1039/C4TB01349B.
Johnston, Robert, Rogelj, Snezna, Harper, Jason C., and Tartis, Michaelann. Fri . "Sol-Generating Chemical Vapor into Liquid (SG-CViL) deposition – a facile method for encapsulation of diverse cell types in silica matrices". United States. doi:10.1039/C4TB01349B. https://www.osti.gov/servlets/purl/1426898.
@article{osti_1426898,
title = {Sol-Generating Chemical Vapor into Liquid (SG-CViL) deposition – a facile method for encapsulation of diverse cell types in silica matrices},
author = {Johnston, Robert and Rogelj, Snezna and Harper, Jason C. and Tartis, Michaelann},
abstractNote = {In nature, cells perform a variety of complex functions such as sensing, catalysis, and energy conversion which hold great potential for biotechnological device construction. However, cellular sensitivity to ex vivo environments necessitates development of bio–nano interfaces which allow integration of cells into devices and maintain their desired functionality. In order to develop such an interface, the use of a novel Sol-Generating Chemical Vapor into Liquid (SG-CViL) deposition process for whole cell encapsulation in silica was explored. In SG-CViL, the high vapor pressure of tetramethyl orthosilicate (TMOS) is utilized to deliver silica into an aqueous medium, creating a silica sol. Cells are then mixed with the resulting silica sol, facilitating encapsulation of cells in silica while minimizing cell contact with the cytotoxic products of silica generating reactions (i.e. methanol), and reduce exposure of cells to compressive stresses induced from silica condensation reactions. Using SG-CVIL, Saccharomyces cerevisiae (S. cerevisiae) engineered with an inducible beta galactosidase system were encapsulated in silica solids and remained both viable and responsive 29 days post encapsulation. By tuning SG-CViL parameters, thin layer silica deposition on mammalian HeLa and U87 human cancer cells was also achieved. Thus, the ability to encapsulate various cell types in either a multi cell (S. cerevisiae) or a thin layer (HeLa and U87 cells) fashion shows the promise of SG-CViL as an encapsulation strategy for generating cell–silica constructs with diverse functions for incorporation into devices for sensing, bioelectronics, biocatalysis, and biofuel applications.},
doi = {10.1039/C4TB01349B},
journal = {Journal of Materials Chemistry. B},
issn = {2050-750X},
number = 6,
volume = 3,
place = {United States},
year = {2014},
month = {12}
}

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