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Title: Conversion of Corn Stover Hydrolysates to Acids: Comparison Between Clostridium carboxidivorans P7 and Microbial Communities Developed from Lake Sediment and an Anaerobic Digester

Abstract

Anaerobic fermentation is an environmentally sustainable technology for converting a variety of feedstocks to biofuels and bioproducts. Considering the complex nature of lignocellulosic hydrolysates, we aimed to investigate product formation from corn stover hydrolysates by using microbial communities under anaerobic conditions. A community developed from lake sediment was able to produce lactic acid from only glucose in the raw or overlimed hydrolysates. Another community from an anaerobic digester, however, was capable of using all hexose and pentose sugars in the raw and undetoxified hydrolysates and released lactic acid at 26.76 g/L. A pure acetogen, Clostridium carboxidivorans P7, was able to grow on the raw and overlimed hydrolysates, too. But the consumption of sugars was minimal and the total released acid concentrations were less than 2 g/L. Next generation sequencing of the enriched community derived from the anaerobic digester revealed the presence of Lactobacillus strains. The predominant species were Lactobacillus parafarraginis (72.6%) and L. buchneri (13.4%). Product titer from using this enriched community can be further enhanced by cultivating at fed-batch or continuous fermentation modes. Results from this study widened the door for producing valuable products from lignocellulosic feedstocks through using mixed cultures.

Authors:
 [1];  [1];  [2];  [3];  [2]
  1. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
  2. Southern Illinois University
  3. Carbondale Community High School
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1426637
Report Number(s):
NREL/JA-5100-71117
Journal ID: ISSN 2190-6815
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Journal Name:
Biomass Conversion and Biorefinery
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2190-6815
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; anaerobic fermentation; lactic acid; Clostridium carboxidivorans p7; microbial community; corn stover hydrolysate

Citation Formats

Chen, Xiaowen, Tucker, Melvin P, Xia, Chunjie, Kumar, Aditi, and Liang, Yanna. Conversion of Corn Stover Hydrolysates to Acids: Comparison Between Clostridium carboxidivorans P7 and Microbial Communities Developed from Lake Sediment and an Anaerobic Digester. United States: N. p., 2017. Web. doi:10.1007/s13399-017-0239-9.
Chen, Xiaowen, Tucker, Melvin P, Xia, Chunjie, Kumar, Aditi, & Liang, Yanna. Conversion of Corn Stover Hydrolysates to Acids: Comparison Between Clostridium carboxidivorans P7 and Microbial Communities Developed from Lake Sediment and an Anaerobic Digester. United States. doi:10.1007/s13399-017-0239-9.
Chen, Xiaowen, Tucker, Melvin P, Xia, Chunjie, Kumar, Aditi, and Liang, Yanna. Wed . "Conversion of Corn Stover Hydrolysates to Acids: Comparison Between Clostridium carboxidivorans P7 and Microbial Communities Developed from Lake Sediment and an Anaerobic Digester". United States. doi:10.1007/s13399-017-0239-9.
@article{osti_1426637,
title = {Conversion of Corn Stover Hydrolysates to Acids: Comparison Between Clostridium carboxidivorans P7 and Microbial Communities Developed from Lake Sediment and an Anaerobic Digester},
author = {Chen, Xiaowen and Tucker, Melvin P and Xia, Chunjie and Kumar, Aditi and Liang, Yanna},
abstractNote = {Anaerobic fermentation is an environmentally sustainable technology for converting a variety of feedstocks to biofuels and bioproducts. Considering the complex nature of lignocellulosic hydrolysates, we aimed to investigate product formation from corn stover hydrolysates by using microbial communities under anaerobic conditions. A community developed from lake sediment was able to produce lactic acid from only glucose in the raw or overlimed hydrolysates. Another community from an anaerobic digester, however, was capable of using all hexose and pentose sugars in the raw and undetoxified hydrolysates and released lactic acid at 26.76 g/L. A pure acetogen, Clostridium carboxidivorans P7, was able to grow on the raw and overlimed hydrolysates, too. But the consumption of sugars was minimal and the total released acid concentrations were less than 2 g/L. Next generation sequencing of the enriched community derived from the anaerobic digester revealed the presence of Lactobacillus strains. The predominant species were Lactobacillus parafarraginis (72.6%) and L. buchneri (13.4%). Product titer from using this enriched community can be further enhanced by cultivating at fed-batch or continuous fermentation modes. Results from this study widened the door for producing valuable products from lignocellulosic feedstocks through using mixed cultures.},
doi = {10.1007/s13399-017-0239-9},
journal = {Biomass Conversion and Biorefinery},
issn = {2190-6815},
number = 1,
volume = 8,
place = {United States},
year = {2017},
month = {1}
}

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