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Title: Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release

Abstract

Thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) has been widely used as a surface coating to thermally control the detachment of adsorbed cells without the need for extreme stimuli such as enzyme treatment. Recently, the use of 2D and 3D scaffolds in controlling cell positioning, growth, spreading, and migration has been of a great interest in tissue engineering and cell biology. We use a PNIPAM polymer surface coating atop a nanostructured linear diffraction grating to controllably change the surface topography of 2D linear structures using temperature stimuli. Neutron reflectometry and surface diffraction are utilized to examine the conformity of the polymer coating to the grating surface, its hydration profile, and its evolution in response to temperature variations. Our results show that, in the collapsed state, the PNIPAM coating conforms to the grating structures and retains a uniform hydration of 63%. In the swollen state, the polymer expands beyond the grating channels and absorbs up to 87% water. Such properties are particularly desirable for 2D cell growth scaffolds with a built-in nonextreme tissue-release mechanism. Indeed, the current system demonstrates advanced performance in the effective alignment of cultured fibroblast cells and the easy release of the cells upon temperature change.

Authors:
 [1];  [2];  [3];  [4];  [4];  [4];  [5];  [6]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Univ. of Maryland, College Park, MD (United States)
  3. Indiana Univ., Bloomington, IN (United States)
  4. Univ. of South Florida, Tampa, FL (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  6. Indiana Univ., Bloomington, IN (United States). Center for the Exploration of Energy and Matter and Physics Dept.; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Neutron Sciences Directorate
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1237172
Alternate Identifier(s):
OSTI ID: 1426595
Report Number(s):
BNL-108299-2015-JA
Journal ID: ISSN 1944-8244; R&D Project: LS001
Grant/Contract Number:  
SC0012704; AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
ACS Applied Materials and Interfaces
Additional Journal Information:
Journal Volume: 7; Journal Issue: 22; Journal ID: ISSN 1944-8244
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; PNIPAM coating; thermoresponsive nanostructured scaffolds; cell alignment and release; neutron relfectrometry

Citation Formats

Zhernenkov, Mikhail, Ashkar, Rana, Feng, Hao, Akintewe, Olukemi O., Gallant, Nathan D., Toomey, Ryan, Ankner, John F., and Pynn, Roger. Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release. United States: N. p., 2015. Web. doi:10.1021/acsami.5b01453.
Zhernenkov, Mikhail, Ashkar, Rana, Feng, Hao, Akintewe, Olukemi O., Gallant, Nathan D., Toomey, Ryan, Ankner, John F., & Pynn, Roger. Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release. United States. https://doi.org/10.1021/acsami.5b01453
Zhernenkov, Mikhail, Ashkar, Rana, Feng, Hao, Akintewe, Olukemi O., Gallant, Nathan D., Toomey, Ryan, Ankner, John F., and Pynn, Roger. 2015. "Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release". United States. https://doi.org/10.1021/acsami.5b01453. https://www.osti.gov/servlets/purl/1237172.
@article{osti_1237172,
title = {Thermoresponsive PNIPAM Coatings on Nanostructured Gratings for Cell Alignment and Release},
author = {Zhernenkov, Mikhail and Ashkar, Rana and Feng, Hao and Akintewe, Olukemi O. and Gallant, Nathan D. and Toomey, Ryan and Ankner, John F. and Pynn, Roger},
abstractNote = {Thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) has been widely used as a surface coating to thermally control the detachment of adsorbed cells without the need for extreme stimuli such as enzyme treatment. Recently, the use of 2D and 3D scaffolds in controlling cell positioning, growth, spreading, and migration has been of a great interest in tissue engineering and cell biology. We use a PNIPAM polymer surface coating atop a nanostructured linear diffraction grating to controllably change the surface topography of 2D linear structures using temperature stimuli. Neutron reflectometry and surface diffraction are utilized to examine the conformity of the polymer coating to the grating surface, its hydration profile, and its evolution in response to temperature variations. Our results show that, in the collapsed state, the PNIPAM coating conforms to the grating structures and retains a uniform hydration of 63%. In the swollen state, the polymer expands beyond the grating channels and absorbs up to 87% water. Such properties are particularly desirable for 2D cell growth scaffolds with a built-in nonextreme tissue-release mechanism. Indeed, the current system demonstrates advanced performance in the effective alignment of cultured fibroblast cells and the easy release of the cells upon temperature change.},
doi = {10.1021/acsami.5b01453},
url = {https://www.osti.gov/biblio/1237172}, journal = {ACS Applied Materials and Interfaces},
issn = {1944-8244},
number = 22,
volume = 7,
place = {United States},
year = {Wed May 20 00:00:00 EDT 2015},
month = {Wed May 20 00:00:00 EDT 2015}
}

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Cited by: 21 works
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Works referencing / citing this record:

The Role of Backbone Hydration of Poly(N-isopropyl acrylamide) Across the Volume Phase Transition Compared to its Monomer
journal, December 2017


A review on thermoresponsive cell culture systems based on poly( N -isopropylacrylamide) and derivatives
journal, July 2017