The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones

Truttmann, Matthias C.; Cruz, Victor E.; Guo, Xuanzong; Engert, Christoph; Schwartz, Thomas U.; Ploegh, Hidde L.; Tan, Man-Wah

doi:10.1371/journal.pgen.1006023

Title: The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones

Journal Article · Tue May 03 00:00:00 EDT 2016 · PLoS Genetics

DOI:https://doi.org/10.1371/journal.pgen.1006023· OSTI ID:1426270

Truttmann, Matthias C. ^[1];

^[2]; Guo, Xuanzong ^[2]; Engert, Christoph ^[2]; Schwartz, Thomas U. ^[2]; Ploegh, Hidde L. ^[3]; Tan, Man-Wah ^[4]

Whitehead Inst. for Biomedical Research, Cambridge, MA (United States)
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Whitehead Inst. for Biomedical Research, Cambridge, MA (United States); Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Genentech, South San Francisco, CA (United States)

Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Inst. of Health

Grant/Contract Number:: P41 GM103403

OSTI ID:: 1426270

Journal Information:: PLoS Genetics, Vol. 12, Issue 5; ISSN 1553-7404

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 29 works

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Caenorhabditis elegans
histones
elongation factors
Pseudomonas aeruginosa
DNA-binding proteins
heat shock response
autoradiography
protein translation

Title: The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones

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References (59)

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