Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Methods and compositions for altering lignin composition in plants

Patent ·
OSTI ID:1425907
The invention provides methods for decreasing lignin content in plants by reducing expression of a folylpolyglutamate synthetase 1 (FPGS1) coding sequence in the plant. Also provided are methods for reducing lignin content in a plant by down-regulation of FPGS1 expression in the plant. Nucleic acid molecules for modulation of FPGS1 expression and transgenic plants the same are also provided. Plants described herein may be used, for example, as improved biofuel feedstock and as highly digestible forage crops. Methods for processing plant tissue and for producing biofuels by utilizing such plants are also provided.
Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-00OR22725
Assignee:
The Samuel Roberts Noble Foundation, Inc. (Ardmore, OK)
Patent Number(s):
9,909,136
Application Number:
14/710,419
OSTI ID:
1425907
Country of Publication:
United States
Language:
English

References (35)

Quantification of plasma S-adenosylmethionine and S-adenosylhomocysteine as their fluorescent 1,N6-etheno derivatives: an adaptation of previously described methodology journal July 2002
Cellulase digestibility of pretreated biomass is limited by cellulose accessibility journal January 2007
Using the Acetyl Bromide Assay To Determine Lignin Concentrations in Herbaceous Plants:  Some Cautionary Notes journal February 1999
Functional analysis of folate polyglutamylation and its essential role in plant metabolism and development journal September 2010
Methionine catabolism in Arabidopsis cells is initiated by a γ-cleavage process and leads to S-methylcysteine and isoleucine syntheses journal October 2006
Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks journal October 2011
Differential expression of genes involved in C1 metabolism and lignin biosynthesis in wooden core and bast tissues of fibre hemp (Cannabis sativa L.) journal February 2008
Loss of function of folylpolyglutamate synthetase 1 reduces lignin content and improves cell wall digestibility in Arabidopsis journal December 2015
Hydrolysis of lignocellulosic materials for ethanol production: a review journal May 2002
New insights into the molecular architecture of hardwood lignins by chemical degradative methods journal March 1995
The Folylpolyglutamate Synthetase Plastidial Isoform Is Required for Postembryonic Root Development in Arabidopsis journal January 2011
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana journal August 2003
Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling book January 2012
Fermentation of lignocellulosic hydrolysates for ethanol production journal April 1996
The maize brown midrib4 (bm4) gene encodes a functional folylpolyglutamate synthase journal January 2015
Collection and Analysis of Expressed Sequence Tags Derived from Laser Capture Microdissected Switchgrass (Panicum virgatum L. Alamo) Vascular Tissues journal February 2010
An associative analysis of gene expression array data journal January 2003
The plastidial folylpolyglutamate synthetase and root apical meristem maintenance journal May 2011
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data journal March 2003
Ethanol Production from Renewable Resources book January 1999
Changes in composition and sugar release across the annual rings of Populus wood and implications on recalcitrance journal January 2011
The Genetics of Lignin Biosynthesis: Connecting Genotype to Phenotype journal December 2010
Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana journal January 2013
Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation journal September 2010
A Gateway Cloning Vector Set for High-Throughput Functional Analysis of Genes in Planta journal September 2003
Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges journal November 1999
Model-based analysis of oligonucleotide arrays: Expression index computation and outlier detection journal January 2001
A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies journal April 2010
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass journal February 2011
Small-scale and automatable high-throughput compositional analysis of biomass journal October 2010
Engineering of a high-throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release journal February 2010
Downregulation of Cinnamyl Alcohol Dehydrogenase (CAD) Leads to Improved Saccharification Efficiency in Switchgrass journal January 2011
Vascular Patterning journal January 2003
Exploration, normalization, and summaries of high density oligonucleotide array probe level data journal April 2003
Colorimetric Method for Determination of Sugars and Related Substances journal March 1956