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Title: Enhanced itaconic acid production in Aspergillus with increased LaeA expression

Abstract

Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.

Inventors:
;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1425892
Patent Number(s):
9,909,152
Application Number:
14/928,511
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Patent
Resource Relation:
Patent File Date: 2015 Oct 30
Country of Publication:
United States
Language:
English

Citation Formats

Dai, Ziyu, and Baker, Scott E. Enhanced itaconic acid production in Aspergillus with increased LaeA expression. United States: N. p., 2018. Web.
Dai, Ziyu, & Baker, Scott E. Enhanced itaconic acid production in Aspergillus with increased LaeA expression. United States.
Dai, Ziyu, and Baker, Scott E. Tue . "Enhanced itaconic acid production in Aspergillus with increased LaeA expression". United States. doi:. https://www.osti.gov/servlets/purl/1425892.
@article{osti_1425892,
title = {Enhanced itaconic acid production in Aspergillus with increased LaeA expression},
author = {Dai, Ziyu and Baker, Scott E.},
abstractNote = {Fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (LaeA), or both, are described. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also described, as are compositions and kits including the disclosed fungi. Further described are Aspergillus terreus fungi overexpressing the LaeA gene and the use of such fungi for the production of itaconic acid.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 06 00:00:00 EST 2018},
month = {Tue Mar 06 00:00:00 EST 2018}
}

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