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Title: Modeling cell activation at ultra-low ligand concentrations

Abstract

Very low (femtomolar) concentrations of isoprenaline, a 2 adrenergic receptor agonist, appear to activate a significant fraction of HEK293 cells grown in 96-well plates. Here, we develop and analyze a mathematical model based on chemical kinetics to determine whether the observed cell activation can be explained by a simple ligand-receptor interaction. Our analysis indicates that a significant fraction of cells can be activated if engagement of one or two receptors is sufficient to trigger a cellular response.

Authors:
 [1];  [1]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1425782
Report Number(s):
LA-UR-18-22083
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biological Science

Citation Formats

Suderman, Ryan T., and Hlavacek, William Scott. Modeling cell activation at ultra-low ligand concentrations. United States: N. p., 2018. Web. doi:10.2172/1425782.
Suderman, Ryan T., & Hlavacek, William Scott. Modeling cell activation at ultra-low ligand concentrations. United States. doi:10.2172/1425782.
Suderman, Ryan T., and Hlavacek, William Scott. Mon . "Modeling cell activation at ultra-low ligand concentrations". United States. doi:10.2172/1425782. https://www.osti.gov/servlets/purl/1425782.
@article{osti_1425782,
title = {Modeling cell activation at ultra-low ligand concentrations},
author = {Suderman, Ryan T. and Hlavacek, William Scott},
abstractNote = {Very low (femtomolar) concentrations of isoprenaline, a 2 adrenergic receptor agonist, appear to activate a significant fraction of HEK293 cells grown in 96-well plates. Here, we develop and analyze a mathematical model based on chemical kinetics to determine whether the observed cell activation can be explained by a simple ligand-receptor interaction. Our analysis indicates that a significant fraction of cells can be activated if engagement of one or two receptors is sufficient to trigger a cellular response.},
doi = {10.2172/1425782},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Mar 12 00:00:00 EDT 2018},
month = {Mon Mar 12 00:00:00 EDT 2018}
}

Technical Report:

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