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Title: The Need for Integrated Approaches in Metabolic Engineering

Abstract

This review highlights state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. We emphasize that a combination of different approaches over multiple time and size scales must b e considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the "system" that is being manipulated: transcriptome, translatome, proteome, or reactome. By bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes.

Authors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1425413
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Cold Spring Harbor Perspectives in Biology
Additional Journal Information:
Journal Volume: 8; Journal Issue: 11; Journal ID: ISSN 1943-0264
Publisher:
Cold Spring Harbor
Country of Publication:
United States
Language:
English

Citation Formats

Lechner, Anna, Brunk, Elizabeth, and Keasling, Jay D. The Need for Integrated Approaches in Metabolic Engineering. United States: N. p., 2016. Web. doi:10.1101/cshperspect.a023903.
Lechner, Anna, Brunk, Elizabeth, & Keasling, Jay D. The Need for Integrated Approaches in Metabolic Engineering. United States. doi:10.1101/cshperspect.a023903.
Lechner, Anna, Brunk, Elizabeth, and Keasling, Jay D. Mon . "The Need for Integrated Approaches in Metabolic Engineering". United States. doi:10.1101/cshperspect.a023903. https://www.osti.gov/servlets/purl/1425413.
@article{osti_1425413,
title = {The Need for Integrated Approaches in Metabolic Engineering},
author = {Lechner, Anna and Brunk, Elizabeth and Keasling, Jay D.},
abstractNote = {This review highlights state-of-the-art procedures for heterologous small-molecule biosynthesis, the associated bottlenecks, and new strategies that have the potential to accelerate future accomplishments in metabolic engineering. We emphasize that a combination of different approaches over multiple time and size scales must b e considered for successful pathway engineering in a heterologous host. We have classified these optimization procedures based on the "system" that is being manipulated: transcriptome, translatome, proteome, or reactome. By bridging multiple disciplines, including molecular biology, biochemistry, biophysics, and computational sciences, we can create an integral framework for the discovery and implementation of novel biosynthetic production routes.},
doi = {10.1101/cshperspect.a023903},
journal = {Cold Spring Harbor Perspectives in Biology},
issn = {1943-0264},
number = 11,
volume = 8,
place = {United States},
year = {2016},
month = {8}
}

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