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Title: Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Abstract

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

Authors:
ORCiD logo [1];  [2]; ORCiD logo [3];  [4];  [4];  [1];  [4];  [3];  [4];  [4];  [5];  [3]; ORCiD logo [6];  [4];  [7];  [4];  [2];  [7];  [2];  [7] more »;  [2];  [8];  [5]; ORCiD logo [9]; ORCiD logo [9] « less
  1. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging
  2. Arizona State Univ., Tempe, AZ (United States)
  3. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Dept. of Physics
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  5. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Photon Science
  6. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Max-Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany)
  7. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  8. Australian National Univ., Canberra, ACT (Australia). Laser Physics Centre, Research School of Physics and Engineering
  9. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. of Hamburg (Germany). Hamburg Center for Ultrafast Imaging; Univ. of Hamburg (Germany). Dept. of Physics
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH); European Union (EU); National Science Foundation (NSF); German Research Foundation (DFG)
OSTI Identifier:
1425348
Grant/Contract Number:  
AC02-76SF00515; P41GM103393; P41RR001209; ERC-Kupper-614507; 1231306
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 51; Journal Issue: 1; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, and Chapman, Henry N. Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. United States: N. p., 2018. Web. doi:10.1107/s1600576717018131.
Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, & Chapman, Henry N. Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. United States. doi:10.1107/s1600576717018131.
Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, and Chapman, Henry N. Thu . "Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals". United States. doi:10.1107/s1600576717018131. https://www.osti.gov/servlets/purl/1425348.
@article{osti_1425348,
title = {Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals},
author = {Awel, Salah and Kirian, Richard A. and Wiedorn, Max O. and Beyerlein, Kenneth R. and Roth, Nils and Horke, Daniel A. and Oberthür, Dominik and Knoska, Juraj and Mariani, Valerio and Morgan, Andrew and Adriano, Luigi and Tolstikova, Alexandra and Xavier, P. Lourdu and Yefanov, Oleksandr and Aquila, Andrew and Barty, Anton and Roy-Chowdhury, Shatabdi and Hunter, Mark S. and James, Daniel and Robinson, Joseph S. and Weierstall, Uwe and Rode, Andrei V. and Bajt, Saša and Küpper, Jochen and Chapman, Henry N.},
abstractNote = {High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.},
doi = {10.1107/s1600576717018131},
journal = {Journal of Applied Crystallography (Online)},
number = 1,
volume = 51,
place = {United States},
year = {Thu Feb 01 00:00:00 EST 2018},
month = {Thu Feb 01 00:00:00 EST 2018}
}

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