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Title: Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study

Abstract

Proteomics studies of fungal systems have progressed dramatically based on the availability of more fungal genome sequences in recent years. Different proteomics strategies have been applied toward characterization of fungal proteome and revealed important gene functions and proteome dynamics. Presented here is the application of shot-gun proteomic technology to study the bio-remediation of environmental hazards by white-rot fungus. Lignin, a naturally abundant component of the plant biomass, is discovered to promote the degradation of Azo dye by white-rot fungus Irpex lacteus CD2 in the lignin/dye/fungus system. Shotgun proteomics technique was used to understand degradation mechanism at the protein level for the lignin/dye/fungus system. Our proteomics study can identify about two thousand proteins (one third of the predicted white-rot fungal proteome) in a single experiment, as one of the most powerful proteomics platforms to study the fungal system to date. The study shows a significant enrichment of oxidoreduction functional category under the dye/lignin combined treatment. An in vitro validation is performed and supports our hypothesis that the synergy of Fenton reaction and manganese peroxidase might play an important role in DR5B dye degradation. The results could guide the development of effective bioremediation strategies and efficient lignocellulosic biomass conversion.

Authors:
 [1];  [2];  [3];  [2];  [4];  [3];  [3];  [4];  [3];  [5]
  1. Huazhong Univ. of Science and Technology, Wuhan (China). School of Life Science and Technology; Texas A & M Univ., College Station, TX (United States). Dept. of Veterinary Pathology
  2. Huazhong Univ. of Science and Technology, Wuhan (China). School of Life Science and Technology; Texas A & M Univ., College Station, TX (United States). Synthetic and Systems Biology Innovation Hub. Dept. of Plant Pathology and Microbiology
  3. Texas A & M Univ., College Station, TX (United States). Synthetic and Systems Biology Innovation Hub. Dept. of Plant Pathology and Microbiology
  4. Huazhong Univ. of Science and Technology, Wuhan (China). School of Life Science and Technology
  5. Texas A & M Univ., College Station, TX (United States). Dept. of Veterinary Pathology. Office of Texas State Chemist
Publication Date:
Research Org.:
Texas A & M Univ., College Station, TX (United States); Huazhong Univ. of Science and Technology, Wuhan (China)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B); National Natural Science Foundation of China (NNSFC); National High-tech Research and Development Program of China; Major State Basic Research Development Program of China
OSTI Identifier:
1425151
Grant/Contract Number:  
EE0006112; EE0007104; 31170104; 2012AA101805; 2014CB138301
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; proteomics; water microbiology

Citation Formats

Sun, Su, Xie, Shangxian, Cheng, Yanbing, Yu, Hongbo, Zhao, Honglu, Li, Muzi, Li, Xiaotong, Zhang, Xiaoyu, Yuan, Joshua S., and Dai, Susie Y. Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study. United States: N. p., 2017. Web. doi:10.1038/s41598-017-10132-4.
Sun, Su, Xie, Shangxian, Cheng, Yanbing, Yu, Hongbo, Zhao, Honglu, Li, Muzi, Li, Xiaotong, Zhang, Xiaoyu, Yuan, Joshua S., & Dai, Susie Y. Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study. United States. doi:10.1038/s41598-017-10132-4.
Sun, Su, Xie, Shangxian, Cheng, Yanbing, Yu, Hongbo, Zhao, Honglu, Li, Muzi, Li, Xiaotong, Zhang, Xiaoyu, Yuan, Joshua S., and Dai, Susie Y. Tue . "Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study". United States. doi:10.1038/s41598-017-10132-4. https://www.osti.gov/servlets/purl/1425151.
@article{osti_1425151,
title = {Enhancement of Environmental Hazard Degradation in the Presence of Lignin: a Proteomics Study},
author = {Sun, Su and Xie, Shangxian and Cheng, Yanbing and Yu, Hongbo and Zhao, Honglu and Li, Muzi and Li, Xiaotong and Zhang, Xiaoyu and Yuan, Joshua S. and Dai, Susie Y.},
abstractNote = {Proteomics studies of fungal systems have progressed dramatically based on the availability of more fungal genome sequences in recent years. Different proteomics strategies have been applied toward characterization of fungal proteome and revealed important gene functions and proteome dynamics. Presented here is the application of shot-gun proteomic technology to study the bio-remediation of environmental hazards by white-rot fungus. Lignin, a naturally abundant component of the plant biomass, is discovered to promote the degradation of Azo dye by white-rot fungus Irpex lacteus CD2 in the lignin/dye/fungus system. Shotgun proteomics technique was used to understand degradation mechanism at the protein level for the lignin/dye/fungus system. Our proteomics study can identify about two thousand proteins (one third of the predicted white-rot fungal proteome) in a single experiment, as one of the most powerful proteomics platforms to study the fungal system to date. The study shows a significant enrichment of oxidoreduction functional category under the dye/lignin combined treatment. An in vitro validation is performed and supports our hypothesis that the synergy of Fenton reaction and manganese peroxidase might play an important role in DR5B dye degradation. The results could guide the development of effective bioremediation strategies and efficient lignocellulosic biomass conversion.},
doi = {10.1038/s41598-017-10132-4},
journal = {Scientific Reports},
number = ,
volume = 7,
place = {United States},
year = {Tue Sep 12 00:00:00 EDT 2017},
month = {Tue Sep 12 00:00:00 EDT 2017}
}

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Works referenced in this record:

Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis
journal, March 2012

  • Fernandez-Fueyo, E.; Ruiz-Duenas, F. J.; Ferreira, P.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 14, p. 5458-5463
  • DOI: 10.1073/pnas.1119912109