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Title: Measuring fluorescence polarization with a dichrometer

Abstract

In this article, a method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.

Authors:
ORCiD logo [1]
  1. Augusta Univ., Augusta, GA (United States). Dept. of Chemistry and Physics; Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Biology; East Carolina Univ., Greenville, NC (United States). Dept. of Physics
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Augusta Univ., Augusta, GA (United States)
OSTI Identifier:
1424976
Report Number(s):
BNL-200007-2018-JAAM
Journal ID: ISSN 0003-2697
Grant/Contract Number:
SC0012704
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Analytical Biochemistry
Additional Journal Information:
Journal Volume: 532; Journal Issue: C; Journal ID: ISSN 0003-2697
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Anisotropy; Circular dichroism; Dichrometer; Linear dichroism; Photoelastic modulator; Polarization ratio

Citation Formats

Sutherland, John C. Measuring fluorescence polarization with a dichrometer. United States: N. p., 2017. Web. doi:10.1016/j.ab.2017.04.001.
Sutherland, John C. Measuring fluorescence polarization with a dichrometer. United States. doi:10.1016/j.ab.2017.04.001.
Sutherland, John C. Thu . "Measuring fluorescence polarization with a dichrometer". United States. doi:10.1016/j.ab.2017.04.001. https://www.osti.gov/servlets/purl/1424976.
@article{osti_1424976,
title = {Measuring fluorescence polarization with a dichrometer},
author = {Sutherland, John C.},
abstractNote = {In this article, a method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.},
doi = {10.1016/j.ab.2017.04.001},
journal = {Analytical Biochemistry},
number = C,
volume = 532,
place = {United States},
year = {Thu Apr 06 00:00:00 EDT 2017},
month = {Thu Apr 06 00:00:00 EDT 2017}
}

Journal Article:
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