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Title: Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists

Abstract

There is a need to develop novel, high-throughput screening and prioritization methods to identify chemicals with adverse estrogen, androgen, and thyroid activity to protect human health and the environment and is of interest to the Endocrine Disruptor Screening Program. The current aim is to explore the utility of zebrafish as a testing paradigm to classify endocrine activity using phenotypically anchored transcriptome profiling. Transcriptome analysis was conducted on embryos exposed to 25 estrogen-, androgen-, or thyroid-active chemicals at a concentration that elicited adverse malformations or mortality at 120 hours post-fertilization in 80% of the animals exposed. Analysis of the top 1000 significant differentially expressed transcripts across all treatments identified a unique transcriptional and phenotypic profile for thyroid hormone receptor agonists, which can be used as a biomarker screen for potential thyroid hormone agonists.

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1424828
Report Number(s):
PNNL-SA-122324
Journal ID: ISSN 0890-6238; 453060036
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Reproductive Toxicology
Additional Journal Information:
Journal Volume: 77; Journal Issue: C; Journal ID: ISSN 0890-6238
Country of Publication:
United States
Language:
English
Subject:
Transcriptomics; zebrafish; phenotypic anchoring; endocrine disruptors; high-throughput; toxcast

Citation Formats

Haggard, Derik E., Noyes, Pamela D., Waters, Katrina M., and Tanguay, Robert L. Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists. United States: N. p., 2018. Web. doi:10.1016/j.reprotox.2018.02.006.
Haggard, Derik E., Noyes, Pamela D., Waters, Katrina M., & Tanguay, Robert L. Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists. United States. doi:10.1016/j.reprotox.2018.02.006.
Haggard, Derik E., Noyes, Pamela D., Waters, Katrina M., and Tanguay, Robert L. Sun . "Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists". United States. doi:10.1016/j.reprotox.2018.02.006.
@article{osti_1424828,
title = {Transcriptomic and phenotypic profiling in developing zebrafish exposed to thyroid hormone receptor agonists},
author = {Haggard, Derik E. and Noyes, Pamela D. and Waters, Katrina M. and Tanguay, Robert L.},
abstractNote = {There is a need to develop novel, high-throughput screening and prioritization methods to identify chemicals with adverse estrogen, androgen, and thyroid activity to protect human health and the environment and is of interest to the Endocrine Disruptor Screening Program. The current aim is to explore the utility of zebrafish as a testing paradigm to classify endocrine activity using phenotypically anchored transcriptome profiling. Transcriptome analysis was conducted on embryos exposed to 25 estrogen-, androgen-, or thyroid-active chemicals at a concentration that elicited adverse malformations or mortality at 120 hours post-fertilization in 80% of the animals exposed. Analysis of the top 1000 significant differentially expressed transcripts across all treatments identified a unique transcriptional and phenotypic profile for thyroid hormone receptor agonists, which can be used as a biomarker screen for potential thyroid hormone agonists.},
doi = {10.1016/j.reprotox.2018.02.006},
journal = {Reproductive Toxicology},
issn = {0890-6238},
number = C,
volume = 77,
place = {United States},
year = {2018},
month = {4}
}