Sugar Release and Growth of Biofuel Crops are Improved by Downregulation of Pectin Biosynthesis

Donohoe, Bryon S; Sykes, Robert W; Gjersing, Erica L; Ziebell, Angela; Turner, Geoffrey; Decker, Steve; Davis, Mark F; Biswal, Ajaya K.; Atmodjo, Melani A.; Li, Mi; Baxter, Holly L.; Yoo, Chang Geun; Pu, Yunqiao; Lee, Yi-Ching; Mazarei, Mitra; Black, Ian M.; Zhang, Ji-Yi; Ramanna, Hema; Bray, Adam L.; King, Zachary R.; LaFayette, Peter R.; Pattathil, Sivakumar; Mohanty, Sushree S.; Ryno, David; Yee, Kelsey; Thompson, Olivia A.; Rodriguez Jr., Miguel; Dumitrache, Alexandru; Natzke, Jace; Winkeler, Kim; Collins, Cassandra; Yang, Xiaohan; Tan, Li; Hahn, Michael G.; Davison, Brian H.; Udvardi, Michael K.; Mielenz, Jonathan R.; Nelson, Richard S.; Parrott, Wayne A.; Ragauskas, Arthur J.; Stewart Jr., C. Neal; Mohnen, Debra

doi:10.1038/nbt.4067

Sugar Release and Growth of Biofuel Crops are Improved by Downregulation of Pectin Biosynthesis

Journal Article · 12 February 2018 · Nature Biotechnology

DOI:https://doi.org/10.1038/nbt.4067· OSTI ID:1424575

Donohoe, Bryon S ^[1]; Sykes, Robert W ^[1]; Gjersing, Erica L ^[1]; Ziebell, Angela ^[1]; Turner, Geoffrey ^[1]; ^[1]; ^[1]; Biswal, Ajaya K. ^[2]; Atmodjo, Melani A. ^[2]; Li, Mi ^[3]; Baxter, Holly L. ^[4]; Yoo, Chang Geun ^[3]; Pu, Yunqiao ^[3]; Lee, Yi-Ching ^[5]; Mazarei, Mitra ^[4]; Black, Ian M. ^[6]; Zhang, Ji-Yi ^[5]; Ramanna, Hema ^[5]; Bray, Adam L. ^[7]; King, Zachary R. ^[7] more »

National Renewable Energy Laboratory (NREL), Golden, CO (United States)
University of Georgia; Oak Ridge National Laboratory
Oak Ridge National Laboratory; UT-ORNL Joint Institute for Biological Sciences
Oak Ridge National Laboratory; University of Tennessee
Oak Ridge National Laboratory; Noble Research Institute
University of Georgia
Oak Ridge National Laboratory; University of Georgia
Oak Ridge National Laboratory
Oak Ridge National Laboratory; ArborGen, Inc.
Oak Ridge National Laboratory; UT-ORNL Joint Institute for Biological Sciences; University of Tennessee

Cell walls in crops and trees have been engineered for production of biofuels and commodity chemicals, but engineered varieties often fail multi-year field trials and are not commercialized. We engineered reduced expression of a pectin biosynthesis gene (Galacturonosyltransferase 4, GAUT4) in switchgrass and poplar, and find that this improves biomass yields and sugar release from biomass processing. Both traits were maintained in a 3-year field trial of GAUT4-knockdown switchgrass, with up to sevenfold increased saccharification and ethanol production and sixfold increased biomass yield compared with control plants. We show that GAUT4 is an a-1,4-galacturonosyltransferase that synthesizes homogalacturonan (HG). Downregulation of GAUT4 reduces HG and rhamnogalacturonan II (RGII), reduces wall calcium and boron, and increases extractability of cell wall sugars. Decreased recalcitrance in biomass processing and increased growth are likely due to reduced HG and RGII cross-linking in the cell wall.

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Office of Biological and Environmental Research; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

DOE Contract Number:: AC36-08GO28308

OSTI ID:: 1424575

Report Number(s):: NREL/JA-2700-70753

Journal Information:: Nature Biotechnology, Vol. 36, Issue 3; ISSN 1087-0156

Publisher:: Springer Nature

Country of Publication:: United States

Language:: English

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