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Title: A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15

Authors:
; ORCiD logo; ORCiD logo
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1424516
Alternate Identifier(s):
OSTI ID: 1397639
Grant/Contract Number:
AC02-06CH11357
Resource Type:
Journal Article: Published Article
Journal Name:
Structure
Additional Journal Information:
Journal Volume: 25; Journal Issue: 3; Related Information: CHORUS Timestamp: 2018-03-06 20:44:22; Journal ID: ISSN 0969-2126
Publisher:
Elsevier
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Powers, Robert E., Gaudet, Rachelle, and Sotomayor, Marcos. A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15. United Kingdom: N. p., 2017. Web. doi:10.1016/j.str.2017.01.014.
Powers, Robert E., Gaudet, Rachelle, & Sotomayor, Marcos. A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15. United Kingdom. doi:10.1016/j.str.2017.01.014.
Powers, Robert E., Gaudet, Rachelle, and Sotomayor, Marcos. Wed . "A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15". United Kingdom. doi:10.1016/j.str.2017.01.014.
@article{osti_1424516,
title = {A Partial Calcium-Free Linker Confers Flexibility to Inner-Ear Protocadherin-15},
author = {Powers, Robert E. and Gaudet, Rachelle and Sotomayor, Marcos},
abstractNote = {},
doi = {10.1016/j.str.2017.01.014},
journal = {Structure},
number = 3,
volume = 25,
place = {United Kingdom},
year = {Wed Mar 01 00:00:00 EST 2017},
month = {Wed Mar 01 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.str.2017.01.014

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  • A study of the permeability of the middle ear-inner ear interface for macromolecules was carried out in chinchillas with open and obstructed eustachian tubes utilizing tritiated human serum albumin and immunoelectrophoresis. Tritiated albumin was placed in the round window niche area or normal animals and animals in which the eustachian tubes had been obstructed for 24 hours or 14 days. The tritiated albumin was allowed to remain in the middle ear cavity for 24 hours, Samples of middle ear effusion, perilymph, blood and cerebrospinal fluid were collected and measured for radioactivity. Radioactivity was demonstrated in the perilymph. Samples of middlemore » ear effusions and perilymph were also studied by immunoelectrophoresis with goat antihuman albumin. Albumin placed in the round window niche of an experimental animal could be recovered unchanged in the perilymph. The results suggest a pathophysiologic explanation for the association of otitis media and sensorineural hearing loss or endolymphatic hydrops.« less
  • On the basis of modeling studies, the (2-deoxy-{alpha}- and {beta}-D-erythro-pentofuranosyl) (thymin-1-yl) alkanes 1a,b and 2a,b were selected as potential conformational probes for altDNA oligonucleotides. A straightforward approach to the synthesis of 1a,b and 2a,b from commercial 2-deoxy-D-ribose (3) and 1-O-methyl-2-deoxy-3,5-di-O-p-toluoyl-D-erythro-pentofuranose (13), respectively, was developed. These nucleoside analogues were converted to the phosphoramidite derivatives 27a,b-30a,b and incorporated into oligonucleotide 31 at predetermined sites and defined internucleotidic motifs. The insertion of 1a,b according to either a (3{prime} {yields} 5{prime})- or a (3{prime} {yields} 3{prime})-internucleotidic polarity produced oligonucleotides exhibiting a slightly higher affinity for their complementary unmodified DNA sequence than for the correspondingmore » RNA sequence (Table 3). Conversely, the incorporation of 2a into 31 according to a (3{prime} {yields} 3{prime})-orientation generated, for the first time, an altDNA oligonucleotide displaying a greater affinity for its complementary unmodified RNA sequence ({Delta}T{sub m} = 6 {degrees}C) than for the corresponding DNA sequence ({Delta}T{sub m} = 10 {degrees}C). This hybrid was, however, thermodynamically less stable than the duplex having unmodified {alpha}-2{prime}-deoxythymidine similarly incorporated into 31 ({Delta}{Delta}T{sub m} = 3 {degrees}C). 29 refs., 2 figs., 3 tabs.« less