Diatom centromeres suggest a mechanism for nuclear DNA acquisition

Diner, Rachel E.; Noddings, Chari M.; Lian, Nathan C.; Kang, Anthony K.; McQuaid, Jeffrey B.; Jablanovic, Jelena; Espinoza, Josh L.; Nguyen, Ngocquynh A.; Anzelmatti, Jr., Miguel A.; Jansson, Jakob; Bielinski, Vincent A.; Karas, Bogumil J.; Dupont, Christopher L.; Allen, Andrew E.; Weyman, Philip D.

doi:10.1073/pnas.1700764114

Title: Diatom centromeres suggest a mechanism for nuclear DNA acquisition

Journal Article · Mon Jul 03 00:00:00 EDT 2017 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1700764114· OSTI ID:1367772

Diner, Rachel E. ^[1]; Noddings, Chari M. ^[2]; Lian, Nathan C. ^[2]; Kang, Anthony K. ^[2]; McQuaid, Jeffrey B. ^[1]; Jablanovic, Jelena ^[3]; Espinoza, Josh L. ^[3]; Nguyen, Ngocquynh A. ^[2]; Anzelmatti, Jr., Miguel A. ^[3]; Jansson, Jakob ^[2]; Bielinski, Vincent A. ^[2]; Karas, Bogumil J. ^[2]; Dupont, Christopher L. ^[3]; Allen, Andrew E. ^[1];

^[2]

Integrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92037,, Microbial and Environmental Genomics Group, J. Craig Venter Institute, La Jolla, CA 92037,
Synthetic Biology and Bioenergy Group, J. Craig Venter Institute, La Jolla, CA 92037
Microbial and Environmental Genomics Group, J. Craig Venter Institute, La Jolla, CA 92037,

Centromeres are essential for cell division and growth in all eukaryotes, and knowledge of their sequence and structure guides the development of artificial chromosomes for functional cellular biology studies. Centromeric proteins are conserved among eukaryotes; however, centromeric DNA sequences are highly variable. We combined forward and reverse genetic approaches with chromatin immunoprecipitation to identify centromeres of the model diatom Phaeodactylum tricornutum. We observed 25 unique centromere sequences typically occurring once per chromosome, a finding that helps to resolve nuclear genome organization and indicates monocentric regional centromeres. Diatom centromere sequences contain low-GC content regions but lack repeats or other conserved sequence features. Native and foreign sequences with similar GC content to P. tricornutum centromeres can maintain episomes and recruit the diatom centromeric histone protein CENH3, suggesting nonnative sequences can also function as diatom centromeres. Thus, simple sequence requirements may enable DNA from foreign sources to persist in the nucleus as extrachromosomal episomes, revealing a potential mechanism for organellar and foreign DNA acquisition.

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Research Organization:: J. Craig Venter Inst., La Jolla, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Gordon and Betty Moore Foundation (United States); National Science Foundation (NSF)

Grant/Contract Number:: SC0008593; GBMF5007; GBMF3828; GBMF5006; MCB-1129303; OCE-1136477

OSTI ID:: 1367772

Alternate ID(s):: OSTI ID: 1423790

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 114 Journal Issue: 29; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 37 works

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59 BASIC BIOLOGICAL SCIENCES
diatom
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episome
centromere
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