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Title: Bringing diffuse X-ray scattering into focus

Abstract

X-ray crystallography is experiencing a renaissance as a method for probing the protein conformational ensemble. The inherent limitations of Bragg analysis, however, which only reveals the mean structure, have given way to a surge in interest in diffuse scattering, which is caused by structure variations. Diffuse scattering is present in all macromolecular crystallography experiments. Recent studies are shedding light on the origins of diffuse scattering in protein crystallography, and provide clues for leveraging diffuse scattering to model protein motions with atomic detail.

Authors:
ORCiD logo [1];  [2]; ORCiD logo [2]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. Univ. of California, San Francisco, CA (United States).
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1422967
Report Number(s):
LA-UR-17-30486
Journal ID: ISSN 0959-440X
Grant/Contract Number:
AC52-06NA25396
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Current Opinion in Structural Biology
Additional Journal Information:
Journal Volume: 50; Journal Issue: C; Journal ID: ISSN 0959-440X
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; Biological Science

Citation Formats

Wall, Michael E., Wolff, Alexander M., and Fraser, James S. Bringing diffuse X-ray scattering into focus. United States: N. p., 2018. Web. doi:10.1016/j.sbi.2018.01.009.
Wall, Michael E., Wolff, Alexander M., & Fraser, James S. Bringing diffuse X-ray scattering into focus. United States. doi:10.1016/j.sbi.2018.01.009.
Wall, Michael E., Wolff, Alexander M., and Fraser, James S. Fri . "Bringing diffuse X-ray scattering into focus". United States. doi:10.1016/j.sbi.2018.01.009. https://www.osti.gov/servlets/purl/1422967.
@article{osti_1422967,
title = {Bringing diffuse X-ray scattering into focus},
author = {Wall, Michael E. and Wolff, Alexander M. and Fraser, James S.},
abstractNote = {X-ray crystallography is experiencing a renaissance as a method for probing the protein conformational ensemble. The inherent limitations of Bragg analysis, however, which only reveals the mean structure, have given way to a surge in interest in diffuse scattering, which is caused by structure variations. Diffuse scattering is present in all macromolecular crystallography experiments. Recent studies are shedding light on the origins of diffuse scattering in protein crystallography, and provide clues for leveraging diffuse scattering to model protein motions with atomic detail.},
doi = {10.1016/j.sbi.2018.01.009},
journal = {Current Opinion in Structural Biology},
number = C,
volume = 50,
place = {United States},
year = {Fri Feb 16 00:00:00 EST 2018},
month = {Fri Feb 16 00:00:00 EST 2018}
}

Journal Article:
Free Publicly Available Full Text
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