Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system

Stickel, Jonathan J.; Adhikari, Birendra; Sievers, David A.; Pellegrino, John

doi:10.1002/jctb.5559

Title: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system

Journal Article · Wed Feb 21 00:00:00 EST 2018 · Journal of Chemical Technology and Biotechnology (1986)

DOI:https://doi.org/10.1002/jctb.5559· OSTI ID:1426636

^[1]; Adhikari, Birendra ^[2];

^[1]; Pellegrino, John ^[2]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ. of Colorado, Boulder, CO (United States)

Abstract BACKGROUND Converting abundant lignocellulosic biomass to sugars as fungible precursors to fuels and chemicals has the potential to diversify the supply chain for those products, but further process improvements are needed to achieve economic viability. In the current work, process intensification of the key enzymatic hydrolysis unit operation is demonstrated by means of a membrane reactor system that was operated continuously. RESULTS Lignocellulosic biomass (pretreated corn stover) and buffered enzyme solution were fed to a continuously stirred‐tank reactor, and clarified sugar solution was withdrawn via a commercial tubular ultrafiltration membrane. The membrane permeance decline and membrane cleaning efficacy were studied and did not vary significantly when increasing fraction insoluble solids (FIS) from 2.5% to 5%. Continuous enzymatic hydrolysis was successfully operated for more than 80 h. A model for the reactor system was able to predict dynamic behavior that was in reasonable agreement with experimental results. CONCLUSION The modeled technical performance of anticipated commercial batch and continuous enzymatic hydrolysis processes were compared and showed that continuous operation would provide at least twice the volumetric productivity for the conditions studied. Further improvements are anticipated by better membrane selection and by increasing FIS. © 2017 Society of Chemical Industry

View Accepted Manuscript (DOE)

View Accepted Manuscript (Publisher)

Cite

Export

Save

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC36-08GO28308; DE‐AC36‐08‐GO28308

OSTI ID:: 1426636

Alternate ID(s):: OSTI ID: 1422626

Report Number(s):: NREL/JA-5100-70067

Journal Information:: Journal of Chemical Technology and Biotechnology (1986), Vol. 93, Issue 5; ISSN 0268-2575

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 15 works

Citation information provided by
Web of Science

References (37)

Enzyme recycling in lignocellulosic biorefineries Jørgensen, Henning; Pinelo, Manuel Biofuels, Bioproducts and Biorefining, Vol. 11, Issue 1 https://doi.org/10.1002/bbb.1724	journal	October 2016
Reactor design for minimizing product inhibition during enzymatic lignocellulose hydrolysis Andrić, Pavle; Meyer, Anne S.; Jensen, Peter A. Biotechnology Advances, Vol. 28, Issue 3 https://doi.org/10.1016/j.biotechadv.2010.02.005	journal	May 2010
Particle concentration and yield stress of biomass slurries during enzymatic hydrolysis at high-solids loadings Roche, Christine M.; Dibble, Clare J.; Knutsen, Jeffrey S. Biotechnology and Bioengineering, Vol. 104, Issue 2 https://doi.org/10.1002/bit.22381	journal	October 2009
Membrane-based recovery of glucose from enzymatic hydrolysis of ionic liquid pretreated cellulose Abels, Christian; Thimm, Kristof; Wulfhorst, Helene Bioresource Technology, Vol. 149 https://doi.org/10.1016/j.biortech.2013.09.012	journal	December 2013
Cellulase Retention and Sugar Removal by Membrane Ultrafiltration During Lignocellulosic Biomass Hydrolysis Knutsen, Jeffrey S.; Davis, Robert H. Applied Biochemistry and Biotechnology, Vol. 114, Issue 1-3 https://doi.org/10.1385/ABAB:114:1-3:585	journal	January 2004
Combined Sedimentation and Filtration Process for Cellulase Recovery During Hydrolysis of Lignocellulosic Biomass Knutsen, Jeffrey S.; Davis, Robert H. Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9 https://doi.org/10.1385/ABAB:98-100:1-9:1161	journal	January 2002
Fractionation of Pre‐Hydrolysis Products from Lignocellulosic Biomass by an Ultrafiltration Ceramic Tubular Membrane Colyar, Kendra R.; Pellegrino, John; Kadam, Kiran Separation Science and Technology, Vol. 43, Issue 3 https://doi.org/10.1080/01496390701812517	journal	February 2008
Techno-economic analysis of a lignocellulosic ethanol biorefinery with ionic liquid pre-treatment Klein-Marcuschamer, Daniel; Simmons, Blake A.; Blanch, Harvey W. Biofuels, Bioproducts and Biorefining, Vol. 5, Issue 5 https://doi.org/10.1002/bbb.303	journal	June 2011
Process Design and Economics for the Conversion of Lignocellulosic Biomass to Hydrocarbons: Dilute-Acid and Enzymatic Deconstruction of Biomass to Sugars and Biological Conversion of Sugars to Hydrocarbons Davis, R.; Tao, L.; Tan, E. C. D. https://doi.org/10.2172/1107470 NREL/TP-5100-60223	report	October 2013
Efficient acid-catalyzed hydrolysis of cellulose in organic electrolyte solutions Zhang, Zehui; Liu, Bing; Zhao, Zongbao (Kent) Polymer Degradation and Stability, Vol. 97, Issue 4 https://doi.org/10.1016/j.polymdegradstab.2012.01.010	journal	April 2012
Kinetic Studies on the Product Inhibition of Enzymatic Lignocellulose Hydrolysis Miao, Yelian; Chen, Jie Yu; Jiang, Xuejian Applied Biochemistry and Biotechnology, Vol. 167, Issue 2 https://doi.org/10.1007/s12010-012-9689-5	journal	May 2012
Design and operation of an integrated membrane reactor for enzymatic cellulose hydrolysis Gan, Q.; Allen, S. J.; Taylor, G. Biochemical Engineering Journal, Vol. 12, Issue 3 https://doi.org/10.1016/S1369-703X(02)00072-4	journal	December 2002
Morphological analyses of natural organic matter (NOM) fouling of low-pressure membranes (MF/UF) Lee, N.; Amy, G.; Croue, J. Journal of Membrane Science, Vol. 261, Issue 1-2 https://doi.org/10.1016/j.memsci.2005.02.039	journal	September 2005
Parameter determination and validation for a mechanistic model of the enzymatic saccharification of cellulose-I _β Nag, Ambarish; Sprague, Michael A.; Griggs, Andrew J. Biotechnology Progress, Vol. 31, Issue 5 https://doi.org/10.1002/btpr.2122	journal	July 2015
Reactor design for minimizing product inhibition during enzymatic lignocellulose hydrolysis: I. Significance and mechanism of cellobiose and glucose inhibition on cellulolytic enzymes Andrić, Pavle; Meyer, Anne S.; Jensen, Peter A. Biotechnology Advances, Vol. 28, Issue 3, p. 308-324 https://doi.org/10.1016/j.biotechadv.2010.01.003	journal	May 2010
Constant flowrate blocking laws and an example of their application to dead-end microfiltration of protein solutions Hlavacek, M.; Bouchet, F. Journal of Membrane Science, Vol. 82, Issue 3 https://doi.org/10.1016/0376-7388(93)85193-Z	journal	July 1993
Dynamic modeling and validation of a lignocellulosic enzymatic hydrolysis process – A demonstration scale study Prunescu, Remus Mihail; Sin, Gürkan Bioresource Technology, Vol. 150 https://doi.org/10.1016/j.biortech.2013.10.029	journal	December 2013
Continuous recycling of enzymes during production of lignocellulosic bioethanol in demonstration scale Haven, Mai Østergaard; Lindedam, Jane; Jeppesen, Martin Dan Applied Energy, Vol. 159 https://doi.org/10.1016/j.apenergy.2015.08.062	journal	December 2015
Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J. Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729 https://doi.org/10.1021/ie801542g	journal	April 2009
Characterization of pilot-scale dilute acid pretreatment performance using deacetylated corn stover Shekiro III, Joseph; Kuhn, Erik M.; Nagle, Nicholas J. Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-23	journal	January 2014
Rheology measurements of a biomass slurry: an inter-laboratory study Stickel, Jonathan J.; Knutsen, Jeffrey S.; Liberatore, Matthew W. Rheologica Acta, Vol. 48, Issue 9 https://doi.org/10.1007/s00397-009-0382-8	journal	September 2009
Fractionating recalcitrant lignocellulose at modest reaction conditions Zhang, Yi-Heng Percival; Ding, Shi-You; Mielenz, Jonathan R. Biotechnology and Bioengineering, Vol. 97, Issue 2 https://doi.org/10.1002/bit.21386	journal	January 2007
Enzymatic lignocellulose hydrolysis: Improved cellulase productivity by insoluble solids recycling Weiss, Noah; Börjesson, Johan; Pedersen, Lars Saaby Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-5	journal	January 2013
Sugar production from wheat straw biomass by alkaline extrusion and enzymatic hydrolysis Coimbra, Michelle Cardoso; Duque, Aleta; Saéz, Felicia Renewable Energy, Vol. 86 https://doi.org/10.1016/j.renene.2015.09.026	journal	February 2016
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production Himmel, M. E.; Ding, S.-Y.; Johnson, D. K. Science, Vol. 315, Issue 5813, p. 804-807 https://doi.org/10.1126/science.1137016	journal	February 2007
Sugar concentration and detoxification of clarified biomass hydrolysate by nanofiltration Malmali, Mohammadmahdi; Stickel, Jonathan J.; Wickramasinghe, S. Ranil Separation and Purification Technology, Vol. 132 https://doi.org/10.1016/j.seppur.2014.06.014	journal	August 2014
Continuous enzymatic cellulose hydrolysis in a tubular membrane bioreactor Bélafi-Bakó, K.; Koutinas, A.; Nemestóthy, N. Enzyme and Microbial Technology, Vol. 38, Issue 1-2 https://doi.org/10.1016/j.enzmictec.2005.05.012	journal	January 2006
A three mechanism model to describe fouling of microfiltration membranes Duclos-Orsello, Chase; Li, Weiyi; Ho, Chia-Chi Journal of Membrane Science, Vol. 280, Issue 1-2 https://doi.org/10.1016/j.memsci.2006.03.005	journal	September 2006
Countercurrent enzymatic saccharification of cellulosic biomass Zentay, Agustin N.; Liang, Chao; Lonkar, Sagar Biomass and Bioenergy, Vol. 90 https://doi.org/10.1016/j.biombioe.2016.03.033	journal	July 2016
The behavior of suspensions and macromolecular solutions in crossflow microfiltration Belfort, Georges; Davis, Robert H.; Zydney, Andrew L. Journal of Membrane Science, Vol. 96, Issue 1-2 https://doi.org/10.1016/0376-7388(94)00119-7	journal	November 1994
A Simplified Method for the Measurement of Insoluble Solids in Pretreated Biomass Slurries Weiss, Noah D.; Stickel, Jonathan J.; Wolfe, Jeffrey L. Applied Biochemistry and Biotechnology, Vol. 162, Issue 4 https://doi.org/10.1007/s12010-009-8806-6	journal	October 2009
Data smoothing and numerical differentiation by a regularization method Stickel, Jonathan J. Computers & Chemical Engineering, Vol. 34, Issue 4 https://doi.org/10.1016/j.compchemeng.2009.10.007	journal	April 2010
Organic solvent pretreatment of lignocellulosic biomass for biofuels and biochemicals: A review Zhang, Ke; Pei, Zhijian; Wang, Donghai Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.08.102	journal	January 2016
Simultaneous saccharification, filtration and fermentation (SSFF): A novel method for bioethanol production from lignocellulosic biomass Ishola, Mofoluwake M.; Jahandideh, Arash; Haidarian, Behroz Bioresource Technology, Vol. 133 https://doi.org/10.1016/j.biortech.2013.01.130	journal	April 2013
A low-cost solid–liquid separation process for enzymatically hydrolyzed corn stover slurries Sievers, David A.; Lischeske, James J.; Biddy, Mary J. Bioresource Technology, Vol. 187 https://doi.org/10.1016/j.biortech.2015.03.087	journal	July 2015
Performance evaluation and fouling analysis for reverse osmosis and nanofiltration membranes during processing of lignocellulosic biomass hydrolysate Gautam, AmitKumar; Menkhaus, Todd J. Journal of Membrane Science, Vol. 451 https://doi.org/10.1016/j.memsci.2013.09.042	journal	February 2014
Investigation of a submerged membrane reactor for continuous biomass hydrolysis Malmali, Mohammadmahdi; Stickel, Jonathan; Wickramasinghe, S. Ranil Food and Bioproducts Processing, Vol. 96 https://doi.org/10.1016/j.fbp.2015.07.001	journal	October 2015

Cited By (1)

A two-phase substrate model for enzymatic hydrolysis of lignocellulose: application to batch and continuous reactors Lischeske, James J.; Stickel, Jonathan J. Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1633-2	journal	December 2019

Similar Records

A two-phase substrate model for enzymatic hydrolysis of lignocellulose: application to batch and continuous reactors

Journal Article · Fri Dec 27 00:00:00 EST 2019 · Biotechnology for Biofuels · OSTI ID:1426636

Lischeske, James J.; Stickel, Jonathan J.

Investigation of a submerged membrane reactor for continuous biomass hydrolysis

Journal Article · Thu Oct 01 00:00:00 EDT 2015 · Food and Bioproducts Processing · OSTI ID:1426636

Malmali, Mohammadmahdi; Stickel, Jonathan; Wickramasinghe, S. Ranil

Development of a Membrane-Based Separation Process for the Continuous Enzymatic Saccharification of Lignocellulosic Biomass; NREL (National Renewable Energy Laboratory)

Conference · Tue Apr 29 00:00:00 EDT 2014 · OSTI ID:1426636

Adhikari, B.; Pellegrino, J.; Stickel, J.; +1 more

Related Subjects

09 BIOMASS FUELS
enzymatic hydrolysis
lignocellulose
continuous reaction
membrane reactor
process intensification

Title: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system: Continuous enzymatic hydrolysis of lignocellulosic biomass in a membrane-reactor system

Citation Formats

References (37)

Cited By (1)

Similar Records

Related Subjects