Pink-beam serial crystallography

Meents, A.; Wiedorn, M. O.; Srajer, V.; Henning, R.; Sarrou, I.; Bergtholdt, J.; Barthelmess, M.; Reinke, P. Y.  A.; Dierksmeyer, D.; Tolstikova, A.; Schaible, S.; Messerschmidt, M.; Ogata, C. M.; Kissick, D. J.; Taft, M. H.; Manstein, D. J.; Lieske, J.; Oberthuer, D.; Fischetti, R. F.; Chapman, H. N.

doi:10.1038/s41467-017-01417-3

Title: Pink-beam serial crystallography

Journal Article · Fri Nov 03 00:00:00 EDT 2017 · Nature Communications

DOI:https://doi.org/10.1038/s41467-017-01417-3· OSTI ID:1422407

^[1];

^[2]; Srajer, V. ^[3]; Henning, R. ^[4]; Sarrou, I. ^[5]; Bergtholdt, J. ^[5]; Barthelmess, M. ^[5]; Reinke, P. Y. A. ^[6]; Dierksmeyer, D. ^[5]; Tolstikova, A. ^[7]; Schaible, S. ^[8]; Messerschmidt, M. ^[9]; Ogata, C. M. ^[10]; Kissick, D. J. ^[10];

^[6]; Manstein, D. J. ^[6]; Lieske, J. ^[8]; Oberthuer, D. ^[5];

^[10];

^[11]

DESY, Hamburg (Germany). Center for Free Electron Laser Science and Photon Science
DESY, Hamburg (Germany). Center for Free Electron Laser Science; University of Hamburg (Germany). Dept. of Physics
Univ. of Chicago, IL (United States). Center for Advanced Radiation Sources
Center for Advanced Radiation Sources
DESY, Hamburg (Germany). Center for Free Electron Laser Science
Institut für Biophysikalische Chemie, Hannover (Germany). Medizinische Hochschule Hannover (MHH)
University of Hamburg (Germany). Dept. of Physics
Deutsches Elektronen Synchrotron (DESY), Hamburg (Germany). Photon Science
National Science Foundation BioXFEL Science and Technology Center, Buffalo, NY (United States)
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
DESY, Hamburg (Germany). Center for Free Electron Laser Science; University of Hamburg (Germany). Dept. of Physics; Centre for Ultrafast Imaging, Hamburg (Germany)

Serial X-ray crystallography allows macromolecular structure determination at both X-ray free electron lasers (XFELs) and, more recently, synchrotron sources. The time resolution for serial synchrotron crystallography experiments has been limited to millisecond timescales with monochromatic beams. The polychromatic, “pink”, beam provides a more than two orders of magnitude increased photon flux and hence allows accessing much shorter timescales in diffraction experiments at synchrotron sources. Here we report the structure determination of two different protein samples by merging pink-beam diffraction patterns from many crystals, each collected with a single 100 ps X-ray pulse exposure per crystal using a setup optimized for very low scattering background. In contrast to experiments with monochromatic radiation, data from only 50 crystals were required to obtain complete datasets. The high quality of the diffraction data highlights the potential of this method for studying irreversible reactions at sub-microsecond timescales using high-brightness X-ray facilities.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division; European Union - Horizon 2020 Research and Innovation Programme; Helmholtz Association of German Research Centres - Virtual Institute; National Institutes of Health (NIH) - National Institute of General Medical Sciences

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1422407

Journal Information:: Nature Communications, Vol. 8, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 76 works

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