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Title: Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture

Abstract

The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized – and confirmed – that co-cultivation under glucose as the sole carbon source would result in a competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because Marinobacter HL-58 was supported by metabolites derived from Halomonas HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships willmore » unfold.« less

Authors:
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Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1422280
Report Number(s):
PNNL-SA-129337
Journal ID: ISSN 2045-2322; 49356; KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Scientific Reports; Journal Volume: 8; Journal Issue: 1
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Khan, Nymul, Maezato, Yukari, McClure, Ryan S., Brislawn, Colin J., Mobberley, Jennifer M., Isern, Nancy, Chrisler, William B., Markillie, Lye Meng, Barney, Brett M., Song, Hyun-Seob, Nelson, William C., and Bernstein, Hans C.. Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture. United States: N. p., 2018. Web. doi:10.1038/s41598-017-18630-1.
Khan, Nymul, Maezato, Yukari, McClure, Ryan S., Brislawn, Colin J., Mobberley, Jennifer M., Isern, Nancy, Chrisler, William B., Markillie, Lye Meng, Barney, Brett M., Song, Hyun-Seob, Nelson, William C., & Bernstein, Hans C.. Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture. United States. doi:10.1038/s41598-017-18630-1.
Khan, Nymul, Maezato, Yukari, McClure, Ryan S., Brislawn, Colin J., Mobberley, Jennifer M., Isern, Nancy, Chrisler, William B., Markillie, Lye Meng, Barney, Brett M., Song, Hyun-Seob, Nelson, William C., and Bernstein, Hans C.. Wed . "Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture". United States. doi:10.1038/s41598-017-18630-1.
@article{osti_1422280,
title = {Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture},
author = {Khan, Nymul and Maezato, Yukari and McClure, Ryan S. and Brislawn, Colin J. and Mobberley, Jennifer M. and Isern, Nancy and Chrisler, William B. and Markillie, Lye Meng and Barney, Brett M. and Song, Hyun-Seob and Nelson, William C. and Bernstein, Hans C.},
abstractNote = {The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized – and confirmed – that co-cultivation under glucose as the sole carbon source would result in a competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because Marinobacter HL-58 was supported by metabolites derived from Halomonas HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships will unfold.},
doi = {10.1038/s41598-017-18630-1},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United States},
year = {Wed Jan 10 00:00:00 EST 2018},
month = {Wed Jan 10 00:00:00 EST 2018}
}