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Title: Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses

Journal Article · · Physical Review Letters
 [1];  [2];  [3];  [4];  [5];  [6];  [3];  [7];  [6];  [8];  [6];  [6];  [9];  [6];  [10];  [3];  [6];  [6];  [11];  [12] more »;  [13];  [3];  [2];  [1] « less
  1. European XFEL GmbH, Schenefeld (Germany)
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Center for Advanced Mathematics for Energy Research Applications, Berkeley, CA (United States)
  3. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  4. La Trobe Univ., Melbourne (Australia)
  5. Uppsala Univ., Uppsala (Sweden); European XFEL GmbH, Schenefeld (Germany)
  6. Uppsala Univ., Uppsala (Sweden)
  7. Arizona State Univ., Tempe, AZ (United States)
  8. Uppsala Univ., Uppsala (Sweden); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  9. Center for Advanced Mathematics for Energy Research Applications, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  10. KTH Royal Institute of Technology, Stockholm (Sweden); Uppsala Univ., Uppsala (Sweden)
  11. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); National Research Nuclear Univ. MePhl (Moscow Engineering Physics Institute), Moscow (Russia)
  12. Brookhaven National Lab. (BNL), Upton, NY (United States)
  13. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Max-Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
+ Show Author Affiliations

We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-05CH11231; ERC-2013-SyG 609920; NSF-1231306; R01GM095583; R01GM109019; SC0012704; AC02-76SF00515
OSTI ID:
1408102
Alternate ID(s):
OSTI ID: 1399167; OSTI ID: 1421825; OSTI ID: 1436269
Report Number(s):
BNL-203624-2018-JAAM; PRLTAO; TRN: US1702931
Journal Information:
Physical Review Letters, Vol. 119, Issue 15; ISSN 0031-9007
Publisher:
American Physical Society (APS)Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 73 works
Citation information provided by
Web of Science

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Correlations in scattered x-ray laser pulses reveal nanoscale structural features of viruses [Supplemental Data]
  • Kurta, Ruslan P.; Donatelli, Jeffrey J.; Yoon, Chun Hong
  • Coherent X-ray Imaging Data Bank (CXIDB), 1 fxs file (55.71 MB), 1 fxs file (32.05 MB) https://doi.org/10.11577/1435599
dataset July 2017
Cryptotomography: Reconstructing 3D Fourier Intensities from Randomly Oriented Single-Shot Diffraction Patterns text January 2010
X-ray cross-correlation analysis applied to disordered two-dimensional systems text January 2012
Toward unsupervised single-shot diffractive imaging of heterogeneous particles using X-ray free-electron lasers text January 2013
A reconstruction algorithm for single-particle diffraction imaging experiments journal August 2009
Cryptotomography: reconstructing 3D Fourier intensities from randomly oriented single-shot diffraction patterns text January 2010
Orientation Determination in Single Particle X-ray Coherent Diffraction Imaging Experiments text January 2013
High-resolution structure of viruses from random diffraction snapshots preprint January 2014

Cited By (34)

Coherence and pulse duration characterization of the PAL-XFEL in the hard X-ray regime journal March 2019
A statistical approach to detect protein complexes at X-ray free electron laser facilities journal December 2018
Free-electron laser data for multiple-particle fluctuation scattering analysis journal October 2018
Design of a liquid cell toward three-dimensional imaging of unidirectionally-aligned particles in solution using X-ray free-electron lasers journal January 2020
Local photo-mechanical stiffness revealed in gold nanoparticles supracrystals by ultrafast small-angle electron diffraction journal March 2019
Three-dimensional optical trapping and orientation of microparticles for coherent X-ray diffraction imaging journal February 2019
Ab initio structure determination from experimental fluctuation X-ray scattering data journal October 2018
Multi-target detection with application to cryo-electron microscopy journal September 2019
Native mass spectrometry provides sufficient ion flux for XFEL single-particle imaging journal April 2019
Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging journal September 2018
Biological single-particle imaging using XFELs – towards the next resolution revolution journal October 2018
Evaluation of the performance of classification algorithms for XFEL single-particle imaging data journal February 2019
Experimental 3D coherent diffractive imaging from photon-sparse random projections journal March 2019
Electrospray sample injection for single-particle imaging with x-ray lasers journal May 2019
Correlations in scattered x-ray laser pulses reveal nanoscale structural features of viruses [Supplemental Data]
  • Kurta, Ruslan P.; Donatelli, Jeffrey J.; Yoon, Chun Hong
  • Coherent X-ray Imaging Data Bank (CXIDB), 1 fxs file (55.71 MB), 1 fxs file (32.05 MB) https://doi.org/10.11577/1435599
dataset July 2017
Colloidal crystal order and structure revealed by tabletop extreme ultraviolet scattering and coherent diffractive imaging journal January 2018
Using convex optimization of autocorrelation with constrained support and windowing for improved phase retrieval accuracy journal January 2018
Supervised classification methods for flash X-ray single particle diffraction imaging journal January 2019
Software Platform for European XFEL: Towards Online Experimental Data Analysis text January 2018
Megahertz single-particle imaging at the European XFEL text January 2020
Angular X-ray Cross-Correlation Analysis (AXCCA): Basic Concepts and Recent Applications to Soft Matter and Nanomaterials journal October 2019
Megahertz single-particle imaging at the European XFEL journal May 2020
Software Platform for European XFEL: Towards Online Experimental Data Analysis journal November 2018
Angular X-ray Cross-Correlation Analysis (AXCCA): Basic Concepts and Recent Applications to Soft Matter and Nanomaterials text January 2019
Single-particle imaging without symmetry constraints at an X-ray free-electron laser text January 2018
Evaluation of the performance of classification algorithms for XFEL single-particle imaging data text January 2019
Considerations for three-dimensional image reconstruction from experimental data in coherent diffractive imaging text January 2018
Evaluation of the performance of classification algorithms for XFEL single-particle imaging data text January 2019
Biological single-particle imaging using XFELs – towards the next resolution revolution text January 2018
Experimental 3D coherent diffractive imaging from photon-sparse random projections text January 2019
Low-signal limit of X-ray single particle diffractive imaging text January 2019
Initial observations of the femtosecond timing jitter at the European XFEL text January 2019
Using Convex Optimization of Autocorrelation with Constrained Support and Windowing for Improved Phase Retrieval Accuracy text January 2018
Local photo-mechanical stiffness revealed in gold nanoparticles supracrystals by ultrafast small-angle electron diffraction text January 2018

Figures / Tables (4)

FIG. 1(p. 3)figure FIG. 1
FIG. 2(p. 3)figure FIG. 2
FIG. 3(p. 4)figure FIG. 3
FIG. 4(p. 4)figure FIG. 4

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