skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock-out mutants in Zea mays

Abstract

Crop yield is critical for human nutrition, yet the underlyingmachinery that ultimately determines yield potential is still notunderstood. Crop productivity under ideal conditions is deter-mined by the efficiency with which plants intercept light, convertit into chemical energy, translocate photosynthates and convertthese to storage products in harvestable organs (Zhuet al., 2010).In many crops, sucrose is the primary form for translocationinside the conduit (i.e. the phloem). A combination of SWEET-mediated efflux from phloem parenchyma and subsequent sec-ondary active sucrose import by SUT sucrose/H+symporters isthought to create the driving force for pressure gradient-drivenphloem transport and retrieval of sucrose leaking along thetranslocation path (Chenet al., 2015a).Sucrose is thought to follow one of three routes during phloemloading: (1) apoplasmic loading via plasma membrane trans-porters, (2) symplasmic loading via diffusion through plasmodes-mata or (3) polymer trapping via enzymatic addition ofgalactose, which is thought to impair back-diffusion throughplasmodesmata (Turgeon & Wolf, 2009; Chenet al., 2015a).Some mechanisms may coexist, as suggested by anatomical stud-ies which have found thin- and thick-walled sieve tubes inmonocots, cell types that may differ regarding the primary load-ing mechanism (Botha, 2013).

Authors:
 [1];  [1];  [2];  [3];  [4];  [3];  [1];  [2]
  1. Institute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf 40225 Germany, Max Planck Institute for Plant Breeding Research, Cologne 50829 Germany, Department of Plant Biology, Carnegie Science, 260 Panama St Stanford CA 94305 USA
  2. Department of Plant Biology, Carnegie Science, 260 Panama St Stanford CA 94305 USA
  3. Department of Genetics, Development, and Cell Biology, Iowa State University, Ames IA 50011 USA
  4. Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln NE 68588 USA
Publication Date:
Research Org.:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1421567
Alternate Identifier(s):
OSTI ID: 1421568; OSTI ID: 1500215
Grant/Contract Number:  
FG02-04ER15542
Resource Type:
Journal Article: Published Article
Journal Name:
New Phytologist
Additional Journal Information:
Journal Name: New Phytologist Journal Volume: 218 Journal Issue: 2; Journal ID: ISSN 0028-646X
Publisher:
Wiley-Blackwell
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bezrutczyk, Margaret, Hartwig, Thomas, Horschman, Marc, Char, Si Nian, Yang, Jinliang, Yang, Bing, Frommer, Wolf B., and Sosso, Davide. Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock-out mutants in Zea mays. United Kingdom: N. p., 2018. Web. doi:10.1111/nph.15021.
Bezrutczyk, Margaret, Hartwig, Thomas, Horschman, Marc, Char, Si Nian, Yang, Jinliang, Yang, Bing, Frommer, Wolf B., & Sosso, Davide. Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock-out mutants in Zea mays. United Kingdom. doi:10.1111/nph.15021.
Bezrutczyk, Margaret, Hartwig, Thomas, Horschman, Marc, Char, Si Nian, Yang, Jinliang, Yang, Bing, Frommer, Wolf B., and Sosso, Davide. Fri . "Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock-out mutants in Zea mays". United Kingdom. doi:10.1111/nph.15021.
@article{osti_1421567,
title = {Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock-out mutants in Zea mays},
author = {Bezrutczyk, Margaret and Hartwig, Thomas and Horschman, Marc and Char, Si Nian and Yang, Jinliang and Yang, Bing and Frommer, Wolf B. and Sosso, Davide},
abstractNote = {Crop yield is critical for human nutrition, yet the underlyingmachinery that ultimately determines yield potential is still notunderstood. Crop productivity under ideal conditions is deter-mined by the efficiency with which plants intercept light, convertit into chemical energy, translocate photosynthates and convertthese to storage products in harvestable organs (Zhuet al., 2010).In many crops, sucrose is the primary form for translocationinside the conduit (i.e. the phloem). A combination of SWEET-mediated efflux from phloem parenchyma and subsequent sec-ondary active sucrose import by SUT sucrose/H+symporters isthought to create the driving force for pressure gradient-drivenphloem transport and retrieval of sucrose leaking along thetranslocation path (Chenet al., 2015a).Sucrose is thought to follow one of three routes during phloemloading: (1) apoplasmic loading via plasma membrane trans-porters, (2) symplasmic loading via diffusion through plasmodes-mata or (3) polymer trapping via enzymatic addition ofgalactose, which is thought to impair back-diffusion throughplasmodesmata (Turgeon & Wolf, 2009; Chenet al., 2015a).Some mechanisms may coexist, as suggested by anatomical stud-ies which have found thin- and thick-walled sieve tubes inmonocots, cell types that may differ regarding the primary load-ing mechanism (Botha, 2013).},
doi = {10.1111/nph.15021},
journal = {New Phytologist},
issn = {0028-646X},
number = 2,
volume = 218,
place = {United Kingdom},
year = {2018},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1111/nph.15021

Citation Metrics:
Cited by: 6 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Characterizing Regulatory and Functional Differentiation between Maize Mesophyll and Bundle Sheath Cells by Transcriptomic Analysis
journal, July 2012

  • Chang, Yao-Ming; Liu, Wen-Yu; Shih, Arthur Chun-Chieh
  • Plant Physiology, Vol. 160, Issue 1
  • DOI: 10.1104/pp.112.203810

TAL effector driven induction of a SWEET gene confers susceptibility to bacterial blight of cotton
journal, May 2017

  • Cox, Kevin L.; Meng, Fanhong; Wilkins, Katherine E.
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/ncomms15588

AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes
journal, October 2016

  • Kanno, Yuri; Oikawa, Takaya; Chiba, Yasutaka
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms13245

Sucrose transporter1 functions in phloem loading in maize leaves
journal, January 2009

  • Slewinski, Thomas L.; Meeley, Robert; Braun, David M.
  • Journal of Experimental Botany, Vol. 60, Issue 3
  • DOI: 10.1093/jxb/ern335

Functional role of oligomerization for bacterial and plant SWEET sugar transporter family
journal, September 2013

  • Xuan, Y. H.; Hu, Y. B.; Chen, L. -Q.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 39
  • DOI: 10.1073/pnas.1311244110

The developmental dynamics of the maize leaf transcriptome
journal, October 2010

  • Li, Pinghua; Ponnala, Lalit; Gandotra, Neeru
  • Nature Genetics, Vol. 42, Issue 12, p. 1060-1067
  • DOI: 10.1038/ng.703

STAR: ultrafast universal RNA-seq aligner
journal, October 2012


Genome-wide efficient mixed-model analysis for association studies
journal, June 2012

  • Zhou, Xiang; Stephens, Matthew
  • Nature Genetics, Vol. 44, Issue 7
  • DOI: 10.1038/ng.2310

Expression of photosynthesis gene-promoter fusions in leaf epidermal cells of transgenic tobacco plants
journal, July 1991


PLINK: A Tool Set for Whole-Genome Association and Population-Based Linkage Analyses
journal, September 2007

  • Purcell, Shaun; Neale, Benjamin; Todd-Brown, Kathe
  • The American Journal of Human Genetics, Vol. 81, Issue 3
  • DOI: 10.1086/519795

Maize association population: a high-resolution platform for quantitative trait locus dissection
journal, November 2005


The mechanism of phloem loading in rice (Oryza sativa)
journal, March 2012


Phloem Transport: Cellular Pathways and Molecular Trafficking
journal, June 2009


Sucrose Efflux Mediated by SWEET Proteins as a Key Step for Phloem Transport
journal, December 2011


Xanthomonas axonopodis Virulence Is Promoted by a Transcription Activator-Like Effector–Mediated Induction of a SWEET Sugar Transporter in Cassava
journal, November 2014

  • Cohn, Megan; Bart, Rebecca S.; Shybut, Mikel
  • Molecular Plant-Microbe Interactions, Vol. 27, Issue 11
  • DOI: 10.1094/MPMI-06-14-0161-R

Improving Photosynthetic Efficiency for Greater Yield
journal, June 2010


Antisense Expression of a Rice Sucrose Transporter OsSUT1 in Rice (Oryza sativa L.)
journal, October 2001

  • Ishimaru, Ken; Hirose, Tatsurou; Aoki, Naohiro
  • Plant and Cell Physiology, Vol. 42, Issue 10
  • DOI: 10.1093/pcp/pce148

Plant MetGenMAP: An Integrative Analysis System for Plant Systems Biology
journal, October 2009

  • Joung, Je-Gun; Corbett, Anthony M.; Fellman, Shanna Moore
  • Plant Physiology, Vol. 151, Issue 4
  • DOI: 10.1104/pp.109.145169

Comparative analyses of C4 and C3 photosynthesis in developing leaves of maize and rice
journal, October 2014

  • Wang, Lin; Czedik-Eysenberg, Angelika; Mertz, Rachel A.
  • Nature Biotechnology, Vol. 32, Issue 11
  • DOI: 10.1038/nbt.3019

High-throughput RNA extraction from plant samples based on homogenisation by reciprocal shaking in the presence of a mixture of sand and glass beads
journal, September 1996

  • Eggermont, Kristel; Goderis, Inge J.; Broekaert, Willem F.
  • Plant Molecular Biology Reporter, Vol. 14, Issue 3
  • DOI: 10.1007/BF02671663

Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters
journal, November 2000

  • Gottwald, J. R.; Krysan, P. J.; Young, J. C.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 25
  • DOI: 10.1073/pnas.250473797

The Arabidopsis vacuolar sugar transporter SWEET2 limits carbon sequestration from roots and restricts Pythium infection
journal, September 2015

  • Chen, Hsin-Yi; Huh, Jung-Hyun; Yu, Ya-Chi
  • The Plant Journal, Vol. 83, Issue 6
  • DOI: 10.1111/tpj.12948

Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning.
journal, January 1994


Sugar transporters for intercellular exchange and nutrition of pathogens
journal, November 2010

  • Chen, Li-Qing; Hou, Bi-Huei; Lalonde, Sylvie
  • Nature, Vol. 468, Issue 7323
  • DOI: 10.1038/nature09606

Understanding and manipulating sucrose phloem loading, unloading, metabolism, and signalling to enhance crop yield and food security
journal, December 2013

  • Braun, D. M.; Wang, L.; Ruan, Y. -L.
  • Journal of Experimental Botany, Vol. 65, Issue 7
  • DOI: 10.1093/jxb/ert416

Sucrose Transporter ZmSut1 Expression and Localization Uncover New Insights into Sucrose Phloem Loading
journal, September 2016

  • Baker, R. Frank; Leach, Kristen A.; Boyer, Nathanial R.
  • Plant Physiology, Vol. 172, Issue 3
  • DOI: 10.1104/pp.16.00884

Mechanism of Substrate Translocation in an Alternating Access Transporter
journal, March 2017


Molecular mechanism of substrate recognition and transport by the AtSWEET13 sugar transporter
journal, September 2017

  • Han, Lei; Zhu, Yongping; Liu, Min
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 38
  • DOI: 10.1073/pnas.1709241114

Antisense suppression of the rice transporter gene, OsSUT1, leads to impaired grain filling and germination but does not affect photosynthesis
journal, January 2002

  • Scofield, Graham N.; Hirose, Tatsuro; Gaudron, Judy A.
  • Functional Plant Biology, Vol. 29, Issue 7
  • DOI: 10.1071/PP01204

SWEET17, a Facilitative Transporter, Mediates Fructose Transport across the Tonoplast of Arabidopsis Roots and Leaves
journal, December 2013

  • Guo, Woei-Jiun; Nagy, Reka; Chen, Hsin-Yi
  • Plant Physiology, Vol. 164, Issue 2
  • DOI: 10.1104/pp.113.232751

Seed filling in domesticated maize and rice depends on SWEET-mediated hexose transport
journal, November 2015

  • Sosso, Davide; Luo, Dangping; Li, Qin-Bao
  • Nature Genetics, Vol. 47, Issue 12
  • DOI: 10.1038/ng.3422

Structure and function of SemiSWEET and SWEET sugar transporters
journal, August 2015


SWEET11 and 15 as key players in seed filling in rice
journal, February 2018

  • Yang, Jungil; Luo, Dangping; Yang, Bing
  • New Phytologist, Vol. 218, Issue 2
  • DOI: 10.1111/nph.15004

Essential Role of Sugar Transporter OsSWEET11 During the Early Stage of Rice Grain Filling
journal, March 2017

  • Ma, Lai; Zhang, Dechun; Miao, Qisong
  • Plant and Cell Physiology, Vol. 58, Issue 5
  • DOI: 10.1093/pcp/pcx040

Carbohydrate Export from the Leaf: A Highly Regulated Process and Target to Enhance Photosynthesis and Productivity: Figure 1.
journal, October 2010

  • Ainsworth, Elizabeth A.; Bush, Daniel R.
  • Plant Physiology, Vol. 155, Issue 1
  • DOI: 10.1104/pp.110.167684

An Agrobacterium -delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize
journal, September 2016

  • Char, Si Nian; Neelakandan, Anjanasree K.; Nahampun, Hartinio
  • Plant Biotechnology Journal, Vol. 15, Issue 2
  • DOI: 10.1111/pbi.12611

Developmental dynamics of Kranz cell transcriptional specificity in maize leaf reveals early onset of C4-related processes
journal, April 2014

  • Tausta, S. Lori; Li, Pinghua; Si, Yaqing
  • Journal of Experimental Botany, Vol. 65, Issue 13
  • DOI: 10.1093/jxb/eru152

Independent and Parallel Evolution of New Genes by Gene Duplication in Two Origins of C4 Photosynthesis Provides New Insight into the Mechanism of Phloem Loading in C4 Species
journal, March 2016

  • Emms, David M.; Covshoff, Sarah; Hibberd, Julian M.
  • Molecular Biology and Evolution, Vol. 33, Issue 7
  • DOI: 10.1093/molbev/msw057

Transport of Sugars
journal, June 2015


Leaf Fructose Content Is Controlled by the Vacuolar Transporter SWEET17 in Arabidopsis
journal, April 2013


Nectar secretion requires sucrose phosphate synthases and the sugar transporter SWEET9
journal, March 2014

  • Lin, I. Winnie; Sosso, Davide; Chen, Li-Qing
  • Nature, Vol. 508, Issue 7497
  • DOI: 10.1038/nature13082

The H + -Sucrose Cotransporter NtSUT1 Is Essential for Sugar Export from Tobacco Leaves
journal, September 1998

  • Bürkle, Lukas; Hibberd, Julian M.; Quick, W. Paul
  • Plant Physiology, Vol. 118, Issue 1
  • DOI: 10.1104/pp.118.1.59

Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing
journal, January 1995


Freeze-quenched maize mesophyll and bundle sheath separation uncovers bias in previous tissue-specific RNA-Seq data
journal, January 2017

  • Denton, Alisandra K.; Maß, Janina; Külahoglu, Canan
  • Journal of Experimental Botany, Vol. 68, Issue 2
  • DOI: 10.1093/jxb/erw463

Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014


Overexpression of the Vacuolar Sugar Carrier AtSWEET16 Modifies Germination, Growth, and Stress Tolerance in Arabidopsis
journal, September 2013

  • Klemens, Patrick A. W.; Patzke, Kathrin; Deitmer, Joachim
  • Plant Physiology, Vol. 163, Issue 3
  • DOI: 10.1104/pp.113.224972

    Works referencing / citing this record:

    The transcriptome of zinc deficient maize roots and its relationship to DNA methylation loss
    journal, December 2018


    Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
    journal, March 2019

    • de Haro, Luis Alejandro; Arellano, Sofía Maité; Novák, Ondrej
    • BMC Plant Biology, Vol. 19, Issue 1
    • DOI: 10.1186/s12870-019-1709-y

    Midrib Sucrose Accumulation and Sugar Transporter Gene Expression in YCS-Affected Sugarcane Leaves
    journal, June 2019

    • Marquardt, Annelie; Henry, Robert J.; Botha, Frederik C.
    • Tropical Plant Biology, Vol. 12, Issue 3
    • DOI: 10.1007/s12042-019-09221-7

    Common metabolic networks contribute to carbon sink strength of sorghum internodes: implications for bioenergy improvement
    journal, November 2019


    Midrib Sucrose Accumulation and Sugar Transporter Gene Expression in YCS-Affected Sugarcane Leaves
    journal, June 2019

    • Marquardt, Annelie; Henry, Robert J.; Botha, Frederik C.
    • Tropical Plant Biology, Vol. 12, Issue 3
    • DOI: 10.1007/s12042-019-09221-7

    The transcriptome of zinc deficient maize roots and its relationship to DNA methylation loss
    journal, December 2018


    Mal de Río Cuarto virus infection causes hormone imbalance and sugar accumulation in wheat leaves
    journal, March 2019

    • de Haro, Luis Alejandro; Arellano, Sofía Maité; Novák, Ondrej
    • BMC Plant Biology, Vol. 19, Issue 1
    • DOI: 10.1186/s12870-019-1709-y

    Common metabolic networks contribute to carbon sink strength of sorghum internodes: implications for bioenergy improvement
    journal, November 2019