Improvements in serial femtosecond crystallography of photosystem II by optimizing crystal uniformity using microseeding procedures

Ibrahim, Mohamed; Chatterjee, Ruchira; Hellmich, Julia; Tran, Rosalie; Bommer, Martin; Yachandra, Vittal K.; Yano, Junko; Kern, Jan; Zouni, Athina

doi:10.1063/1.4919741

Title: Improvements in serial femtosecond crystallography of photosystem II by optimizing crystal uniformity using microseeding procedures

Journal Article · Wed Apr 29 00:00:00 EDT 2015 · Structural Dynamics

DOI:https://doi.org/10.1063/1.4919741· OSTI ID:1190859

Ibrahim, Mohamed ^[1]; Chatterjee, Ruchira ^[2]; Hellmich, Julia ^[1]; Tran, Rosalie ^[2]; Bommer, Martin ^[3]; Yachandra, Vittal K. ^[4]; Yano, Junko ^[2]; Kern, Jan ^[2]; Zouni, Athina ^[1]

Humboldt Univ. of Berlin, Berlin (Germany); Technische Univ. Berlin (Germany). Max-Volmer-Lab. fuer Biophysikalische Chemie
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Div.
Humboldt Univ. of Berlin, Berlin (Germany)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

In photosynthesis, photosystem II (PSII) is the multi-subunit membrane protein complex that catalyzes photo-oxidation of water into dioxygen through the oxygen evolving complex (OEC). To understand the water oxidation reaction, it is important to get structural information about the transient and intermediate states of the OEC in the dimeric PSII core complex (dPSIIcc). In recent times, femtosecond X-ray pulses from the free electron laser (XFEL) are being used to obtain X-ray diffraction (XRD) data of dPSIIcc microcrystals at room temperature that are free of radiation damage. In our experiments at the XFEL, we used an electrospun liquid microjet setup that requires microcrystals less than 40 μm in size. In this study, we explored various microseeding techniques to get a high yield of monodisperse uniform-sized microcrystals. Monodisperse microcrystals of dPSIIcc of uniform size were a key to improve the stability of the jet and the quality of XRD data obtained at the XFEL. This was evident by an improvement of the quality of the datasets obtained, from 6.5 Å, using crystals grown without the micro seeding approach, to 4.5 Å using crystals generated with the new method.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231; GM055302

OSTI ID:: 1190859

Alternate ID(s):: OSTI ID: 1421052; OSTI ID: 1512111

Journal Information:: Structural Dynamics, Vol. 2, Issue 4; ISSN 2329-7778

Publisher:: American Crystallographic Association/AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 20 works

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Structure of photosystem II and substrate binding at room temperature Young, Iris D.; Ibrahim, Mohamed; Chatterjee, Ruchira Nature, Vol. 540, Issue 7633 https://doi.org/10.1038/nature20161	journal	November 2016
Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II Sierra, Raymond G.; Gati, Cornelius; Laksmono, Hartawan Nature Methods, Vol. 13, Issue 1 https://doi.org/10.1038/nmeth.3667	journal	November 2015
Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers Fuller, Franklin D.; Gul, Sheraz; Chatterjee, Ruchira Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4195	journal	February 2017
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Supersaturation-controlled microcrystallization and visualization analysis for serial femtosecond crystallography Lee, Dan Bi; Kim, Jong-Min; Seok, Jong Hyeon Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-01143	text	January 2018

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