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Title: Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass

Abstract

Availability, cost and efficiency of microbial enzymes for lignocellulose bioconversion are central to sustainable biomass ethanol technology. Fungi enriched from decaying biomass and surface soil mixture displayed an array of strong cellulolytic and xylanolytic activities. Strains SG2 and SG4 produced a promising array of cellulolytic and xylanolytic enzymes including β-glucosidase, usually low in cultures of Trichoderma species. Nucleotide sequence analysis of internal transcribed spacer 2 (ITS2) region of rRNA gene revealed that strains SG2 and SG4 are closely related to Trichoderma inhamatum, Trichoderma piluliferum and Trichoderma aureoviride. Trichoderma sp. SG2 correspondingly displayed as much as 9.84±1.12, 48.02±2.53 and 30.10±1.11 units mL-1 of cellulase, xylanase and β-glucosidase. Ten times dilution of culture supernatant of strain SG2 revealed that activities were about 5.34, 8.45, and 2.05 orders of magnitude higher than observed in crude culture filtrate for cellulase, xylanase, and β-glucosidase respectively, indicating that more enzymes are present to contact with substrates in biomass sacharification. In parallel experiments Trichoderma species SG2 and SG4 produced more β-glucosidase than the industrial strain Trichoderma reesei RUT-C30. Results indicate that strains SG2 and SG4 have potential for low cost in-house production of primary lignocellulose-hydrolyzing enzymes for production of biomass saccharides and biofuel in the field.

Authors:
 [1]
  1. Auburn Univ., Montgomery, AL (United States)
Publication Date:
Research Org.:
Auburn Univ., Montgomery, AL (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1419492
Grant/Contract Number:  
EE0003132
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Applied Biochemistry and Biotechnology
Additional Journal Information:
Journal Volume: 174; Journal Issue: 4; Journal ID: ISSN 0273-2289
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Cellulase; xylanase; β-glucosidase; β-xylosidae; biomass saccharides; lignocellulose; bioprocessing; biofuel

Citation Formats

Okeke, Benedict C. Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass. United States: N. p., 2014. Web. doi:10.1007/s12010-014-1121-x.
Okeke, Benedict C. Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass. United States. doi:10.1007/s12010-014-1121-x.
Okeke, Benedict C. Sun . "Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass". United States. doi:10.1007/s12010-014-1121-x. https://www.osti.gov/servlets/purl/1419492.
@article{osti_1419492,
title = {Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass},
author = {Okeke, Benedict C.},
abstractNote = {Availability, cost and efficiency of microbial enzymes for lignocellulose bioconversion are central to sustainable biomass ethanol technology. Fungi enriched from decaying biomass and surface soil mixture displayed an array of strong cellulolytic and xylanolytic activities. Strains SG2 and SG4 produced a promising array of cellulolytic and xylanolytic enzymes including β-glucosidase, usually low in cultures of Trichoderma species. Nucleotide sequence analysis of internal transcribed spacer 2 (ITS2) region of rRNA gene revealed that strains SG2 and SG4 are closely related to Trichoderma inhamatum, Trichoderma piluliferum and Trichoderma aureoviride. Trichoderma sp. SG2 correspondingly displayed as much as 9.84±1.12, 48.02±2.53 and 30.10±1.11 units mL-1 of cellulase, xylanase and β-glucosidase. Ten times dilution of culture supernatant of strain SG2 revealed that activities were about 5.34, 8.45, and 2.05 orders of magnitude higher than observed in crude culture filtrate for cellulase, xylanase, and β-glucosidase respectively, indicating that more enzymes are present to contact with substrates in biomass sacharification. In parallel experiments Trichoderma species SG2 and SG4 produced more β-glucosidase than the industrial strain Trichoderma reesei RUT-C30. Results indicate that strains SG2 and SG4 have potential for low cost in-house production of primary lignocellulose-hydrolyzing enzymes for production of biomass saccharides and biofuel in the field.},
doi = {10.1007/s12010-014-1121-x},
journal = {Applied Biochemistry and Biotechnology},
number = 4,
volume = 174,
place = {United States},
year = {Sun Aug 17 00:00:00 EDT 2014},
month = {Sun Aug 17 00:00:00 EDT 2014}
}

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Cited by: 4 works
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