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Title: Effects of Atmospheric Pressure Plasmas on Isolated and Cellular DNA—A Review

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Journal Article: Published Article
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International Journal of Molecular Sciences
Additional Journal Information:
Journal Volume: 16; Journal Issue: 12; Related Information: CHORUS Timestamp: 2018-01-31 08:59:19; Journal ID: ISSN 1422-0067
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Arjunan, Krishna, Sharma, Virender, and Ptasinska, Sylwia. Effects of Atmospheric Pressure Plasmas on Isolated and Cellular DNA—A Review. Switzerland: N. p., 2015. Web. doi:10.3390/ijms16022971.
Arjunan, Krishna, Sharma, Virender, & Ptasinska, Sylwia. Effects of Atmospheric Pressure Plasmas on Isolated and Cellular DNA—A Review. Switzerland. doi:10.3390/ijms16022971.
Arjunan, Krishna, Sharma, Virender, and Ptasinska, Sylwia. 2015. "Effects of Atmospheric Pressure Plasmas on Isolated and Cellular DNA—A Review". Switzerland. doi:10.3390/ijms16022971.
title = {Effects of Atmospheric Pressure Plasmas on Isolated and Cellular DNA—A Review},
author = {Arjunan, Krishna and Sharma, Virender and Ptasinska, Sylwia},
abstractNote = {},
doi = {10.3390/ijms16022971},
journal = {International Journal of Molecular Sciences},
number = 12,
volume = 16,
place = {Switzerland},
year = 2015,
month = 1

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Publisher's Version of Record at 10.3390/ijms16022971

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Cited by: 24works
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  • Lately, the atmospheric pressure jet type corona plasma, which has been typically driven by dc to low frequency (LF: several tens of kHz), is often generated by using radio frequency of 13.56 MHz. Yet, the relationship between the plasma and its driving frequency has seldom been investigated. Hence, in this study, dependence of the atmospheric pressure corona plasma characteristics on the driving frequency was explored experimentally from LF to rf (5 kHz-13.56 MHz). The plasmas generated by the driving frequency under 2 MHz were cylindrical shape of several tens of millimeters long while the 13.56 MHz plasma is spherical andmore » a few millimeters long. As the driving frequency was increased, the plasma length became shortened. At the lower driving frequencies (below 2 MHz), the plasmas existed as positive streamer and negative glow for each half period of the applied voltage, but the discharge was more continuous in time for the 13.56 MHz plasma. It was inferred from the measured I-V curves that the higher driving frequency induced higher discharge currents, and the gas temperature was increased as the driving frequency was increased.« less
  • Gas discharge plasmas formed at atmospheric pressure and near room temperature have recently been shown as a promising tool for cancer treatment. The mechanism of the plasma action is attributed to generation of reactive oxygen and nitrogen species, electric fields, charges, and photons. The relative importance of different modes of action of atmospheric-pressure plasmas depends on the process parameters and specific treatment objects. Hence, an in-depth understanding of biological mechanisms that underpin plasma-induced death in cancer cells is required to optimise plasma processing conditions. Here, the intracellular factors involved in the observed anti-cancer activity in melanoma Mel007 cells are studied,more » focusing on the effect of the plasma treatment dose on the expression of tumour suppressor protein TP73. Over-expression of TP73 causes cell growth arrest and/or apoptosis, and hence can potentially be targeted to enhance killing efficacy and selectivity of the plasma treatment. It is shown that the plasma treatment induces dose-dependent up-regulation of TP73 gene expression, resulting in significantly elevated levels of TP73 RNA and protein in plasma-treated melanoma cells. Silencing of TP73 expression by means of RNA interference inhibited the anticancer effects of the plasma, similar to the effect of caspase inhibitor z-VAD or ROS scavenger N-acetyl cysteine. These results confirm the role of TP73 protein in dose-dependent regulation of anticancer activity of atmospheric-pressure plasmas.« less
  • Various mechanisms, including increases in lipid peroxidation, have been proposed to account for metal-induced cellular injury. By comparing several metals in the same cell population, it is possible to determine whether a correlation exists between ability to produce cell injury and ability to alter parameters pertaining to a particular mechanism. Of particular interest in this study was the relation between metal-induced cytotoxicity and increases in lipid peroxidation. The effects of Cr, Mn, Zn, Ni, Pb, Se, V, Fe, Cd, Hg, and Cu, at final concentrations of 1 to 1000, on the viability of isolated hepatocytes were therefore examined bymore » assessing the loss of intracellular K/sup +/ and aspartate aminotransferase (AST). Simultaneously, the ability of the metals to induce lipid peroxidation, as measured by an increase in thiobarbituric acid (TBA) reactants, was assessed. Hg and Cu required the lowest concentration to produce cellular injury, while Cd produced less dramatic changes in cell viability and Fe at 1000 produced only a small decrease in intracellular K/sup +/. The largest absolute increases in lipid peroxidation were found in the presence of V, followed by Fe and Hg, with Cd and Se causing the smallest increase in TBA reactants. These observations suggest that the lipid peroxidation associated with Cd and Hg is not necessarily responsible for the loss of cell viability induced by these two metals.« less
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