Photosystem II Water Oxidation: Mechanism, Efficiency and Flux in Diverse Oxygenic Phototrophs

doi:10.2172/1418262

Title: Photosystem II Water Oxidation: Mechanism, Efficiency and Flux in Diverse Oxygenic Phototrophs

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Abstract

In one year, we pursued four aims: 1) extend the VZAD model to allow analysis of PSII chlorophyll fluorescence emission as modulated by interaction with the WOC (partial success); 2) compare the solar energy conversion efficiencies of PSII-WOCs from intact cells, isolated thylakoid membranes and PSII core complexes and crystals from cyanobacterium Thermosynechococcus elongatus (collaboration with Lawrence Berkeley National Laboratory; some success after changing collaborator); 3) determine whether PSIIs can store light energy by pumping protons across the thylakoid membrane (PSII-cyclic electron flow) and how it is regulated within the green alga Chlorella ohadii (collaboration with the Hebrew University of Jerusalem; some success); and 4) genetically replace the native PSII-D1 protein subunit from a higher plant with two cyanobacterial D1 isoforms to test whether their functional advantages in growth and photoprotection can be transferred (collaboration with Rutgers University; success).

Authors:

Dismukes, Gerard Charles ^[1]; Ananyev, Gennady ^[1]; Gates, Colin ^[1]

Rutgers Univ., Piscataway, NJ (United States)

Publication Date:: Tue Jan 09 00:00:00 EST 2018

Research Org.:: Rutgers Univ., New Brunswick, NJ (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division

OSTI Identifier:: 1418262

Report Number(s):: DOE-RUTGERS-05354

DOE Contract Number:: SC0005354

Resource Type:: Technical Report

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 14 SOLAR ENERGY; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Photosystem II; bicarbonate; cyclic electron flow; water oxidation; carbon sequestration

Citation Formats


                    Dismukes, Gerard Charles, Ananyev, Gennady, and Gates, Colin. Photosystem II Water Oxidation: Mechanism, Efficiency and Flux in Diverse Oxygenic Phototrophs.  United States: N. p., 2018. 
        Web.  doi:10.2172/1418262.

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                    Dismukes, Gerard Charles, Ananyev, Gennady, & Gates, Colin. Photosystem II Water Oxidation: Mechanism, Efficiency and Flux in Diverse Oxygenic Phototrophs.  United States.  doi:10.2172/1418262.

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                    Dismukes, Gerard Charles, Ananyev, Gennady, and Gates, Colin. Tue .  
        "Photosystem II Water Oxidation: Mechanism, Efficiency and Flux in Diverse Oxygenic Phototrophs".  United States.  
        doi:10.2172/1418262.  https://www.osti.gov/servlets/purl/1418262.

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@article{osti_1418262,

  title        = {Photosystem II Water Oxidation: Mechanism, Efficiency and Flux in Diverse Oxygenic Phototrophs},

  author       = {Dismukes, Gerard Charles and Ananyev, Gennady and Gates, Colin},

  abstractNote = {In one year, we pursued four aims: 1) extend the VZAD model to allow analysis of PSII chlorophyll fluorescence emission as modulated by interaction with the WOC (partial success); 2) compare the solar energy conversion efficiencies of PSII-WOCs from intact cells, isolated thylakoid membranes and PSII core complexes and crystals from cyanobacterium Thermosynechococcus elongatus (collaboration with Lawrence Berkeley National Laboratory; some success after changing collaborator); 3) determine whether PSIIs can store light energy by pumping protons across the thylakoid membrane (PSII-cyclic electron flow) and how it is regulated within the green alga Chlorella ohadii (collaboration with the Hebrew University of Jerusalem; some success); and 4) genetically replace the native PSII-D1 protein subunit from a higher plant with two cyanobacterial D1 isoforms to test whether their functional advantages in growth and photoprotection can be transferred (collaboration with Rutgers University; success).},

  doi          = {10.2172/1418262},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jan 09 00:00:00 EST 2018},

  month        = {Tue Jan 09 00:00:00 EST 2018}

}

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DOI: 10.2172/1418262

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