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Title: Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Abstract

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

Authors:
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Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1418182
Grant/Contract Number:
AC02-76SF00515; P41GM103393; P41RR001209
Resource Type:
Journal Article: Published Article
Journal Name:
Journal of Applied Crystallography
Additional Journal Information:
Journal Volume: 51; Journal Issue: 1; Related Information: CHORUS Timestamp: 2018-05-18 12:26:15; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
Denmark
Language:
English

Citation Formats

Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, and Chapman, Henry N. Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Denmark: N. p., 2018. Web. doi:10.1107/S1600576717018131.
Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, & Chapman, Henry N. Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals. Denmark. doi:10.1107/S1600576717018131.
Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, and Chapman, Henry N. Thu . "Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals". Denmark. doi:10.1107/S1600576717018131.
@article{osti_1418182,
title = {Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals},
author = {Awel, Salah and Kirian, Richard A. and Wiedorn, Max O. and Beyerlein, Kenneth R. and Roth, Nils and Horke, Daniel A. and Oberthür, Dominik and Knoska, Juraj and Mariani, Valerio and Morgan, Andrew and Adriano, Luigi and Tolstikova, Alexandra and Xavier, P. Lourdu and Yefanov, Oleksandr and Aquila, Andrew and Barty, Anton and Roy-Chowdhury, Shatabdi and Hunter, Mark S. and James, Daniel and Robinson, Joseph S. and Weierstall, Uwe and Rode, Andrei V. and Bajt, Saša and Küpper, Jochen and Chapman, Henry N.},
abstractNote = {High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.},
doi = {10.1107/S1600576717018131},
journal = {Journal of Applied Crystallography},
number = 1,
volume = 51,
place = {Denmark},
year = {Thu Feb 01 00:00:00 EST 2018},
month = {Thu Feb 01 00:00:00 EST 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1107/S1600576717018131

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