skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Interpretation of solution scattering data from lipid nanodiscs

Abstract

The structural information contained in solution scattering data from empty lipid nanodiscs is examined in the context of a multi-component geometric model. X-ray scattering data were collected on nanodiscs of different compositions at scattering vector magnitudes up to 2.0 Å −1 . Through the calculation of the partial form factor for each of the nanodisc components before the isotropic average, structural parameters in the model were correlated to the features observed in the X-ray scattering data and to the corresponding distance distribution function. It is shown that, in general, the features at ∼0.3–0.6 Å −1 in the scattering data correlate to the bilayer structure. The data also support the argument that the elliptical shape of nanodiscs found in model fitting is physical, rather than an artefact due to the nanodisc size distribution. The lipid chain packing peak at ∼1.5 Å −1 is visible in the data and reflects the lipid bilayer phase transition. The shape change in the distance distribution function across the phase transition suggests that the nanodiscs are more circular in the fluid phase. The implication of these findings for model fitting of empty and protein-loaded nanodiscs is discussed.

Authors:
 [1];  [1]; ORCiD logo [1]
  1. Brookhaven National Lab. (BNL), Upton, NY (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Inst. of Health (NIH) (United States)
OSTI Identifier:
1418181
Alternate Identifier(s):
OSTI ID: 1433974
Report Number(s):
BNL-203509-2018-JAAM
Journal ID: ISSN 1600-5767
Grant/Contract Number:
SC0012704; P41 GM111244; S10 OD012331; KP1605010; AC02-98CH10886
Resource Type:
Journal Article: Published Article
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 51; Journal Issue: 1; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; lipid nanodiscs; solution scattering; small-angle X-ray scattering; wide-angle X-ray scattering; SAXS/WAXS; modelling

Citation Formats

Graziano, Vito, Miller, Lisa, and Yang, Lin. Interpretation of solution scattering data from lipid nanodiscs. United States: N. p., 2018. Web. doi:10.1107/S1600576717018441.
Graziano, Vito, Miller, Lisa, & Yang, Lin. Interpretation of solution scattering data from lipid nanodiscs. United States. doi:10.1107/S1600576717018441.
Graziano, Vito, Miller, Lisa, and Yang, Lin. Thu . "Interpretation of solution scattering data from lipid nanodiscs". United States. doi:10.1107/S1600576717018441.
@article{osti_1418181,
title = {Interpretation of solution scattering data from lipid nanodiscs},
author = {Graziano, Vito and Miller, Lisa and Yang, Lin},
abstractNote = {The structural information contained in solution scattering data from empty lipid nanodiscs is examined in the context of a multi-component geometric model. X-ray scattering data were collected on nanodiscs of different compositions at scattering vector magnitudes up to 2.0 Å −1 . Through the calculation of the partial form factor for each of the nanodisc components before the isotropic average, structural parameters in the model were correlated to the features observed in the X-ray scattering data and to the corresponding distance distribution function. It is shown that, in general, the features at ∼0.3–0.6 Å −1 in the scattering data correlate to the bilayer structure. The data also support the argument that the elliptical shape of nanodiscs found in model fitting is physical, rather than an artefact due to the nanodisc size distribution. The lipid chain packing peak at ∼1.5 Å −1 is visible in the data and reflects the lipid bilayer phase transition. The shape change in the distance distribution function across the phase transition suggests that the nanodiscs are more circular in the fluid phase. The implication of these findings for model fitting of empty and protein-loaded nanodiscs is discussed.},
doi = {10.1107/S1600576717018441},
journal = {Journal of Applied Crystallography (Online)},
number = 1,
volume = 51,
place = {United States},
year = {Thu Feb 01 00:00:00 EST 2018},
month = {Thu Feb 01 00:00:00 EST 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1107/S1600576717018441

Save / Share: