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Title: Biogenesis of zinc storage granules in Drosophila melanogaster

Abstract

Membrane transporters and sequestration mechanisms concentrate metal ions differentially into discrete subcellular microenvironments for use in protein cofactors, signalling, storage or excretion. Here we identify zinc storage granules as the insect's major zinc reservoir in principal Malpighian tubule epithelial cells of Drosophila melanogaster. The concerted action of Adaptor Protein-3, Rab32, HOPS and BLOC complexes as well as of the white-scarlet (ABCG2-like) and ZnT35C (ZnT2/ZnT3/ZnT8-like) transporters is required for zinc storage granule biogenesis. Due to lysosome-related organelle defects caused by mutations in the homologous human genes, patients with Hermansky–Pudlak syndrome may lack zinc granules in beta pancreatic cells, intestinal paneth cells and presynaptic vesicles of hippocampal mossy fibers.

Authors:
 [1]; ORCiD logo [1];  [2];  [2];  [3];  [1]; ORCiD logo [1]
  1. Centro de Investigación y de Estudios Avanzados del Inst. Politécnico Nacional, Ciudad de México (Mexico). Dept. de Fisiología, Biofísica y Neurociencias
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
  3. Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Ciudad de México (Mexico). Dept. de Biotecnología y Bioingeniería
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1418066
Alternate Identifier(s):
OSTI ID: 1468689
Grant/Contract Number:  
AC02-76SF00515; P41GM103393
Resource Type:
Journal Article: Published Article
Journal Name:
Journal of Experimental Biology
Additional Journal Information:
Journal Volume: 221; Journal Issue: 6; Journal ID: ISSN 0022-0949
Publisher:
The Company of Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AP-3 complex; Eye color mutants; ICP-OES; Malpighian tubules; Synchrotron; Zincosomes

Citation Formats

Tejeda-Guzmán, Carlos, Rosas-Arellano, Abraham, Kroll, Thomas, Webb, Samuel M., Barajas-Aceves, Martha, Osorio, Beatriz, and Missirlis, Fanis. Biogenesis of zinc storage granules in Drosophila melanogaster. United States: N. p., 2018. Web. doi:10.1242/jeb.168419.
Tejeda-Guzmán, Carlos, Rosas-Arellano, Abraham, Kroll, Thomas, Webb, Samuel M., Barajas-Aceves, Martha, Osorio, Beatriz, & Missirlis, Fanis. Biogenesis of zinc storage granules in Drosophila melanogaster. United States. doi:10.1242/jeb.168419.
Tejeda-Guzmán, Carlos, Rosas-Arellano, Abraham, Kroll, Thomas, Webb, Samuel M., Barajas-Aceves, Martha, Osorio, Beatriz, and Missirlis, Fanis. Wed . "Biogenesis of zinc storage granules in Drosophila melanogaster". United States. doi:10.1242/jeb.168419.
@article{osti_1418066,
title = {Biogenesis of zinc storage granules in Drosophila melanogaster},
author = {Tejeda-Guzmán, Carlos and Rosas-Arellano, Abraham and Kroll, Thomas and Webb, Samuel M. and Barajas-Aceves, Martha and Osorio, Beatriz and Missirlis, Fanis},
abstractNote = {Membrane transporters and sequestration mechanisms concentrate metal ions differentially into discrete subcellular microenvironments for use in protein cofactors, signalling, storage or excretion. Here we identify zinc storage granules as the insect's major zinc reservoir in principal Malpighian tubule epithelial cells of Drosophila melanogaster. The concerted action of Adaptor Protein-3, Rab32, HOPS and BLOC complexes as well as of the white-scarlet (ABCG2-like) and ZnT35C (ZnT2/ZnT3/ZnT8-like) transporters is required for zinc storage granule biogenesis. Due to lysosome-related organelle defects caused by mutations in the homologous human genes, patients with Hermansky–Pudlak syndrome may lack zinc granules in beta pancreatic cells, intestinal paneth cells and presynaptic vesicles of hippocampal mossy fibers.},
doi = {10.1242/jeb.168419},
journal = {Journal of Experimental Biology},
number = 6,
volume = 221,
place = {United States},
year = {Wed Jan 24 00:00:00 EST 2018},
month = {Wed Jan 24 00:00:00 EST 2018}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1242/jeb.168419

Citation Metrics:
Cited by: 1 work
Citation information provided by
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