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Title: Crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of PqqB from Pseudomonas putida

Abstract

PqqB is an enzyme involved in the biosynthesis of pyrroloquinoline quinone and a distal member of the metallo-β-lactamase (MBL) superfamily. PqqB lacks two residues in the conserved signature motif HxHxDH that makes up the key metal-chelating elements that can bind up to two metal ions at the active site of MBLs and other members of its superfamily. Here, we report crystal structures of PqqB bound to Mn2+, Mg2+, Cu2+, and Zn2+. These structures demonstrate that PqqB can still bind metal ions at the canonical MBL active site. The fact that PqqB can adapt its side chains to chelate a wide spectrum of metal ions with different coordination features on a uniform main chain scaffold demonstrates its metal-binding plasticity. This plasticity may provide insights into the structural basis of promiscuous activities found in ensembles of metal complexes within this superfamily. Furthermore, PqqB belongs to a small subclass of MBLs that contain an additional CxCxxC motif that binds a structural Zn2+. Our data support a key role for this motif in dimerization.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1418055
Resource Type:
Journal Article
Resource Relation:
Journal Name: JBIC Journal of Biological Inorganic Chemistry; Journal Volume: 22; Journal Issue: 7
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Tu, Xiongying, Latham, John A., Klema, Valerie J., Evans, Robert L., Li, Chao, Klinman, Judith P., and Wilmot, Carrie M.. Crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of PqqB from Pseudomonas putida. United States: N. p., 2017. Web. doi:10.1007/s00775-017-1486-8.
Tu, Xiongying, Latham, John A., Klema, Valerie J., Evans, Robert L., Li, Chao, Klinman, Judith P., & Wilmot, Carrie M.. Crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of PqqB from Pseudomonas putida. United States. doi:10.1007/s00775-017-1486-8.
Tu, Xiongying, Latham, John A., Klema, Valerie J., Evans, Robert L., Li, Chao, Klinman, Judith P., and Wilmot, Carrie M.. Sat . "Crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of PqqB from Pseudomonas putida". United States. doi:10.1007/s00775-017-1486-8.
@article{osti_1418055,
title = {Crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of PqqB from Pseudomonas putida},
author = {Tu, Xiongying and Latham, John A. and Klema, Valerie J. and Evans, Robert L. and Li, Chao and Klinman, Judith P. and Wilmot, Carrie M.},
abstractNote = {PqqB is an enzyme involved in the biosynthesis of pyrroloquinoline quinone and a distal member of the metallo-β-lactamase (MBL) superfamily. PqqB lacks two residues in the conserved signature motif HxHxDH that makes up the key metal-chelating elements that can bind up to two metal ions at the active site of MBLs and other members of its superfamily. Here, we report crystal structures of PqqB bound to Mn2+, Mg2+, Cu2+, and Zn2+. These structures demonstrate that PqqB can still bind metal ions at the canonical MBL active site. The fact that PqqB can adapt its side chains to chelate a wide spectrum of metal ions with different coordination features on a uniform main chain scaffold demonstrates its metal-binding plasticity. This plasticity may provide insights into the structural basis of promiscuous activities found in ensembles of metal complexes within this superfamily. Furthermore, PqqB belongs to a small subclass of MBLs that contain an additional CxCxxC motif that binds a structural Zn2+. Our data support a key role for this motif in dimerization.},
doi = {10.1007/s00775-017-1486-8},
journal = {JBIC Journal of Biological Inorganic Chemistry},
number = 7,
volume = 22,
place = {United States},
year = {Sat Aug 19 00:00:00 EDT 2017},
month = {Sat Aug 19 00:00:00 EDT 2017}
}