Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630

DeLorenzo, Drew M.; Henson, William R.; Moon, Tae Seok

doi:10.1021/acssynbio.7b00192

Title: Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630

Journal Article · Wed Jul 26 00:00:00 EDT 2017 · ACS Synthetic Biology

DOI:https://doi.org/10.1021/acssynbio.7b00192· OSTI ID:1417982

DeLorenzo, Drew M. ^[1]; Henson, William R. ^[1];

^[1]

Washington Univ., St. Louis, MO (United States)

Rhodococcus opacus PD630 is a non-model, gram positive bacterium that possesses desirable traits for biomass conversion, including consumption capabilities for lignocellulose-based sugars and toxic lignin-derived aromatic compounds, significant triacylglycerol accumulation, relatively rapid growth rate, and genetic tractability. However, few genetic elements have been directly characterized in R. opacus, limiting its application for lignocellulose bioconversion. Here, we report the characterization and development of genetic tools for tunable gene expression in R. opacus, including: 1) six fluorescent reporters for quantifying promoter output, 2) three chemically inducible promoters for variable gene expression, and 3) two classes of metabolite sensors derived from native R. opacus promoters that detect nitrogen levels or aromatic compounds. Using these tools, we also provide insights into native aromatic consumption pathways in R. opacus. Overall, this work expands the ability to control and characterize gene expression in R. opacus for future lignocellulose-based fuel and chemical production.

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Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0012705

OSTI ID:: 1417982

Journal Information:: ACS Synthetic Biology, Vol. 6, Issue 10; ISSN 2161-5063

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 31 works

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Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains Li, Xiaolu; He, Yucai; Zhang, Libing Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1395-x	journal	March 2019
Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds Anthony, Winston E.; Carr, Rhiannon R.; DeLorenzo, Drew M. Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1535-3	journal	August 2019
Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630 DeLorenzo, Drew M.; Moon, Tae Seok Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-24486-w	journal	April 2018
Lipid metabolism of phenol-tolerant Rhodococcus opacus strains for lignin bioconversion Henson, William R.; Hsu, Fong-Fu; Dantas, Gautam Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1337-z	journal	December 2018

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