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Title: Evaluation of light energy to H 2 energy conversion efficiency in thin films of cyanobacteria and green alga under photoautotrophic conditions

Abstract

Cyanobacteria and green algae harness solar energy to split water and to fix CO 2. Under specific conditions, they are capable of photoproduction of molecular hydrogen (H 2). This study compares the light-energy-to-hydrogen-energy conversion efficiency (LHCE) in two heterocystous, N 2-fixing cyanobacteria (wild-type Calothrix sp. strain 336/3 and the ΔhupL mutant of Anabaena sp. strain PCC 7120) and in the sulfur-deprived green alga, Chlamydomonas reinhardtii strain CC-124, after entrapment of the cells in thin Ca 2+-alginate films. The experiments, performed under photoautotrophic conditions, showed higher LHCEs in the cyanobacteria as compared to the green alga. The highest efficiency of ca. 2.5% was obtained in films of the entrapped ΔhupL strain under low light condition (2.9 W m -2). Calothrix sp. 336/3 films produced H 2 with a maximum efficiency of 0.6% under 2.9 W m -2, while C. reinhardtii films produced H 2 most efficiently under moderate light (0.14% at 12.1 W m -2). Exposure of the films to light above 16 W m -2 led to noticeable oxidative stress in all three strains, which increased with light intensity. The presence of oxidative stress was confirmed by increased (i) degradation of chlorophylls and some structural carotenoids (such as β-carotene), (ii)more » production of hydroxylated carotenoids (such as zeaxanthin), and (iii) carbonylation of proteins. We conclude that the H 2 photoproduction efficiency in immobilized algae and cyanobacteria can be further improved by entrapping cultures in immobilization matrices with increased permeability for gases, especially oxygen, while matrices with low porosity produced increased amounts of xanthophylls and other antioxidant compounds.« less

Authors:
ORCiD logo [1];  [1];  [2]; ORCiD logo [1]
  1. University of Turku (Finland). Molecular Plant Biology, Department of Biochemistry
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE). NREL Emeritus Program
OSTI Identifier:
1417328
Report Number(s):
NREL/JA-2700-70297
Journal ID: ISSN 2211-9264
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 28; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
08 HYDROGEN; 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; 30 DIRECT ENERGY CONVERSION; Hydrogen photoproduction; Thin-layer immobilization; Microalgae; Cyanobacteria; Oxidative stress; Carotenoids

Citation Formats

Kosourov, Sergey, Murukesan, Gayathri, Seibert, Michael, and Allahverdiyeva, Yagut. Evaluation of light energy to H2 energy conversion efficiency in thin films of cyanobacteria and green alga under photoautotrophic conditions. United States: N. p., 2017. Web. doi:10.1016/j.algal.2017.09.027.
Kosourov, Sergey, Murukesan, Gayathri, Seibert, Michael, & Allahverdiyeva, Yagut. Evaluation of light energy to H2 energy conversion efficiency in thin films of cyanobacteria and green alga under photoautotrophic conditions. United States. doi:10.1016/j.algal.2017.09.027.
Kosourov, Sergey, Murukesan, Gayathri, Seibert, Michael, and Allahverdiyeva, Yagut. Sat . "Evaluation of light energy to H2 energy conversion efficiency in thin films of cyanobacteria and green alga under photoautotrophic conditions". United States. doi:10.1016/j.algal.2017.09.027. https://www.osti.gov/servlets/purl/1417328.
@article{osti_1417328,
title = {Evaluation of light energy to H2 energy conversion efficiency in thin films of cyanobacteria and green alga under photoautotrophic conditions},
author = {Kosourov, Sergey and Murukesan, Gayathri and Seibert, Michael and Allahverdiyeva, Yagut},
abstractNote = {Cyanobacteria and green algae harness solar energy to split water and to fix CO2. Under specific conditions, they are capable of photoproduction of molecular hydrogen (H2). This study compares the light-energy-to-hydrogen-energy conversion efficiency (LHCE) in two heterocystous, N2-fixing cyanobacteria (wild-type Calothrix sp. strain 336/3 and the ΔhupL mutant of Anabaena sp. strain PCC 7120) and in the sulfur-deprived green alga, Chlamydomonas reinhardtii strain CC-124, after entrapment of the cells in thin Ca2+-alginate films. The experiments, performed under photoautotrophic conditions, showed higher LHCEs in the cyanobacteria as compared to the green alga. The highest efficiency of ca. 2.5% was obtained in films of the entrapped ΔhupL strain under low light condition (2.9 W m-2). Calothrix sp. 336/3 films produced H2 with a maximum efficiency of 0.6% under 2.9 W m-2, while C. reinhardtii films produced H2 most efficiently under moderate light (0.14% at 12.1 W m-2). Exposure of the films to light above 16 W m-2 led to noticeable oxidative stress in all three strains, which increased with light intensity. The presence of oxidative stress was confirmed by increased (i) degradation of chlorophylls and some structural carotenoids (such as β-carotene), (ii) production of hydroxylated carotenoids (such as zeaxanthin), and (iii) carbonylation of proteins. We conclude that the H2 photoproduction efficiency in immobilized algae and cyanobacteria can be further improved by entrapping cultures in immobilization matrices with increased permeability for gases, especially oxygen, while matrices with low porosity produced increased amounts of xanthophylls and other antioxidant compounds.},
doi = {10.1016/j.algal.2017.09.027},
journal = {Algal Research},
number = C,
volume = 28,
place = {United States},
year = {Sat Oct 14 00:00:00 EDT 2017},
month = {Sat Oct 14 00:00:00 EDT 2017}
}

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