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Title: PlantCV v2: Image analysis software for high-throughput plant phenotyping

Journal Article · · PeerJ
DOI:https://doi.org/10.7717/peerj.4088· OSTI ID:1417015
 [1];  [1];  [1];  [1];  [2];  [1];  [3];  [1];  [1];  [3];  [4];  [5];  [6];  [7];  [8];  [9];  [10];  [11];  [12]
  1. Donald Danforth Plant Science Center, St. Louis, MO (United States)
  2. Donald Danforth Plant Science Center, St. Louis, MO (United States); Monsanto Company, St. Louis, MO (United States)
  3. Oklahoma State Univ., Stillwater, OK (United States). Dept. of Plant Biology, Ecology, and Evolution
  4. Donald Danforth Plant Science Center, St. Louis, MO (United States); Washington Univ., St. Louis, MO (United States). Computational and Systems Biology Program
  5. Donald Danforth Plant Science Center, St. Louis, MO (United States); Unidev, St. Louis, MO (United States)
  6. Arkansas State Univ., Jonesboro, AR (United States). Arkansas Biosciences Inst.; Univ. of Georgia, Athens, GA (United States). Dept. of Plant Biology
  7. Donald Danforth Plant Science Center, St. Louis, MO (United States); CiBO Technologies, Cambridge, MA (United States)
  8. Arkansas State Univ., Jonesboro, AR (United States). Arkansas Biosciences Inst., Dept. of Chemistry and Physics
  9. Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Nebraska, Lincoln, NE (United States). Dept. of Agronomy and Horticulture, Center for Plant Science Innovation, Beadle Center for Biotechnology
  10. Cosmos X, Tokyo (Japan)
  11. Donald Danforth Plant Science Center, St. Louis, MO (United States); Oklahoma State Univ., Stillwater, OK (United States). Dept. of Plant Biology, Ecology, and Evolution
  12. Missouri Univ. of Science and Technology, Rolla, MO (United States)

Systems for collecting image data in conjunction with computer vision techniques are a powerful tool for increasing the temporal resolution at which plant phenotypes can be measured non-destructively. Computational tools that are flexible and extendable are needed to address the diversity of plant phenotyping problems. We previously described the Plant Computer Vision (PlantCV) software package, which is an image processing toolkit for plant phenotyping analysis. The goal of the PlantCV project is to develop a set of modular, reusable, and repurposable tools for plant image analysis that are open-source and community-developed. Here in this paper we present the details and rationale for major developments in the second major release of PlantCV. In addition to overall improvements in the organization of the PlantCV project, new functionality includes a set of new image processing and normalization tools, support for analyzing images that include multiple plants, leaf segmentation, landmark identification tools for morphometrics, and modules for machine learning.

Research Organization:
Donald Danforth Plant Science Center, St. Louis, MO (United States); Univ. of Nebraska, Lincoln, NE (United States)
Sponsoring Organization:
USDOE Advanced Research Projects Agency - Energy (ARPA-E); USDOE Office of Science (SC)
Contributing Organization:
National Science Foundation (NSF)
Grant/Contract Number:
AR0000594; SC0014395
OSTI ID:
1417015
Journal Information:
PeerJ, Vol. 5; ISSN 2167-8359
Publisher:
PeerJ Inc.Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 121 works
Citation information provided by
Web of Science

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Morphometric and colourimetric tools to dissect morphological diversity: an application in sweet potato [Ipomoea batatas (L.) Lam.] journal June 2019
Engineering plants for tomorrow: how high-throughput phenotyping is contributing to the development of better crops journal July 2018
3D point cloud data to quantitatively characterize size and shape of shrub crops journal April 2019
Versatile method for quantifying and analyzing morphological differences in experimentally obtained images journal November 2019
3D Digitization in Functional Morphology: Where is the Point of Diminishing Returns? journal June 2019
Pheno-Deep Counter: a unified and versatile deep learning architecture for leaf counting journal September 2018
Contour analysis for interpretable leaf shape category discovery journal October 2019
Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM journal December 2019
Convolutional Neural Net-Based Cassava Storage Root Counting Using Real and Synthetic Images journal November 2019
Non-Invasive Sensing of Nitrogen in Plant Using Digital Images and Machine Learning for Brassica Campestris ssp. Chinensis L. journal May 2019
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Reducing shade avoidance can improve Arabidopsis canopy performance against competitors journal October 2020
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Conventional and hyperspectral time-series imaging of maize lines widely used in field trials journal November 2017
Salt stress under the scalpel – Dissecting the genetics of salt tolerance journal December 2018
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