skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Visualizing chemical functionality in plant cell walls

Abstract

Understanding plant cell wall cross-linking chemistry and polymeric architecture is key to the efficient utilization of biomass in all prospects from rational genetic modification to downstream chemical and biological conversion to produce fuels and value chemicals. In fact, the bulk properties of cell wall recalcitrance are collectively determined by its chemical features over a wide range of length scales from tissue, cellular to polymeric architectures. Microscopic visualization of cell walls from the nanometer to the micrometer scale offers an in situ approach to study their chemical functionality considering its spatial and chemical complexity, particularly the capabilities of characterizing biomass non-destructively and in real-time during conversion processes. Microscopic characterization has revealed heterogeneity in the distribution of chemical features, which would otherwise be hidden in bulk analysis. Key microscopic features include cell wall type, wall layering, and wall composition - especially cellulose and lignin distributions. Microscopic tools, such as atomic force microscopy, stimulated Raman scattering microscopy, and fluorescence microscopy, have been applied to investigations of cell wall structure and chemistry from the native wall to wall treated by thermal chemical pretreatment and enzymatic hydrolysis. While advancing our current understanding of plant cell wall recalcitrance and deconstruction, microscopic tools with improved spatial resolutionmore » will steadily enhance our fundamental understanding of cell wall function.« less

Authors:
ORCiD logo [1];  [1];  [2]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
  2. Michigan State Univ., East Lansing, MI (United States). Department of Plant Biology
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1416720
Report Number(s):
NREL/JA-2700-70484
Journal ID: ISSN 1754-6834
Grant/Contract Number:  
AC36-08GO28308; FC02-07ER64494
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; plant cell wall; cell wall imaging; biomass recalcitrance; bioenergy; lignocellulosic biomass; stimulated raman scattering; atomic force microscopy; fluorescence; fluorescence lifetime imaging microscopy

Citation Formats

Zeng, Yining, Himmel, Michael E., and Ding, Shi-You. Visualizing chemical functionality in plant cell walls. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0953-3.
Zeng, Yining, Himmel, Michael E., & Ding, Shi-You. Visualizing chemical functionality in plant cell walls. United States. doi:10.1186/s13068-017-0953-3.
Zeng, Yining, Himmel, Michael E., and Ding, Shi-You. Thu . "Visualizing chemical functionality in plant cell walls". United States. doi:10.1186/s13068-017-0953-3. https://www.osti.gov/servlets/purl/1416720.
@article{osti_1416720,
title = {Visualizing chemical functionality in plant cell walls},
author = {Zeng, Yining and Himmel, Michael E. and Ding, Shi-You},
abstractNote = {Understanding plant cell wall cross-linking chemistry and polymeric architecture is key to the efficient utilization of biomass in all prospects from rational genetic modification to downstream chemical and biological conversion to produce fuels and value chemicals. In fact, the bulk properties of cell wall recalcitrance are collectively determined by its chemical features over a wide range of length scales from tissue, cellular to polymeric architectures. Microscopic visualization of cell walls from the nanometer to the micrometer scale offers an in situ approach to study their chemical functionality considering its spatial and chemical complexity, particularly the capabilities of characterizing biomass non-destructively and in real-time during conversion processes. Microscopic characterization has revealed heterogeneity in the distribution of chemical features, which would otherwise be hidden in bulk analysis. Key microscopic features include cell wall type, wall layering, and wall composition - especially cellulose and lignin distributions. Microscopic tools, such as atomic force microscopy, stimulated Raman scattering microscopy, and fluorescence microscopy, have been applied to investigations of cell wall structure and chemistry from the native wall to wall treated by thermal chemical pretreatment and enzymatic hydrolysis. While advancing our current understanding of plant cell wall recalcitrance and deconstruction, microscopic tools with improved spatial resolution will steadily enhance our fundamental understanding of cell wall function.},
doi = {10.1186/s13068-017-0953-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {Thu Nov 30 00:00:00 EST 2017},
month = {Thu Nov 30 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 1 work
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Lignin Depolymerization and Conversion A Review of Thermochemical Methods
journal, November 2010

  • Pandey, M. P.; Kim, C. S.
  • Chemical Engineering & Technology, Vol. 34, Issue 1, p. 29-41
  • DOI: 10.1002/ceat.201000270

Growth of the plant cell wall
journal, November 2005

  • Cosgrove, Daniel J.
  • Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861
  • DOI: 10.1038/nrm1746

Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005

  • Lynd, Lee R.; van Zyl, Willem H.; McBride, John E.
  • Current Opinion in Biotechnology, Vol. 16, Issue 5, p. 577-583
  • DOI: 10.1016/j.copbio.2005.08.009

Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol
journal, December 2005


The Path Forward for Biofuels and Biomaterials
journal, January 2006

  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods
journal, May 1998

  • Mooney, Caitriona A.; Mansfield, Shawn D.; Touhy, Maria G.
  • Bioresource Technology, Vol. 64, Issue 2, p. 113-119
  • DOI: 10.1016/S0960-8524(97)00181-8

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


Cellulase for commodity products from cellulosic biomass
journal, August 1999

  • Himmel, Michael E.; Ruth, Mark F.; Wyman, Charles E.
  • Current Opinion in Biotechnology, Vol. 10, Issue 4, p. 358-364
  • DOI: 10.1016/S0958-1669(99)80065-2

Lignin Biosynthesis
journal, June 2003


Lignification and lignin topochemistry — an ultrastructural view
journal, July 2001


Perspectives and new directions for the production of bioethanol using consolidated bioprocessing of lignocellulose
journal, June 2009


Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014

  • Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
  • Science, Vol. 344, Issue 6185, p. 1246843-1246843
  • DOI: 10.1126/science.1246843