Demonstration of Parallel Algal Processing: Production of Renewable Diesel Blendstock and a High-Value Chemical Intermediate

Knoshaug, Eric P; Mohagheghi, Ali; Nagle, Nicholas J; Stickel, Jonathan J; Dong, Tao; Karp, Eric M; Kruger, Jacob S; Brandner, David; Manker, Lorenz; Rorrer, Nicholas; Hyman, Deborah A; Christensen, Earl D; Pienkos, Philip T

doi:10.1039/C7GC02295F

Demonstration of Parallel Algal Processing: Production of Renewable Diesel Blendstock and a High-Value Chemical Intermediate

Journal Article · 19 December 2017 · Green Chemistry

DOI:https://doi.org/10.1039/C7GC02295F· OSTI ID:1416715

^[1]; Mohagheghi, Ali ^[1]; Nagle, Nicholas J ^[1]; ^[1]; ^[1]; ^[1]; Kruger, Jacob S ^[1]; ^[1]; ^[1]; Rorrer, Nicholas ^[1]; Hyman, Deborah A ^[1]; Christensen, Earl D ^[1]; Pienkos, Philip T ^[1]

National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Co-production of high-value chemicals such as succinic acid from algal sugars is a promising route to enabling conversion of algal lipids to a renewable diesel blendstock. Biomass from the green alga Scenedesmus acutus was acid pretreated and the resulting slurry separated into its solid and liquor components using charged polyamide induced flocculation and vacuum filtration. Over the course of a subsequent 756 hours continuous fermentation of the algal liquor with Actinobacillus succinogenes 130Z, we achieved maximum productivity, process conversion yield, and titer of 1.1 g L-1 h-1, 0.7 g g-1 total sugars, and 30.5 g L-1 respectively. Succinic acid was recovered from fermentation media with a yield of 60% at 98.4% purity while lipids were recovered from the flocculated cake at 83% yield with subsequent conversion through deoxygenation and hydroisomerization to a renewable diesel blendstock. This work is a first-of-its-kind demonstration of a novel integrated conversion process for algal biomass to produce fuel and chemical products of sufficient quality to be blend-ready feedstocks for further processing.

Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

DOE Contract Number:: AC36-08GO28308

OSTI ID:: 1416715

Report Number(s):: NREL/JA-5100-70765; GRCHFJ

Journal Information:: Green Chemistry, Vol. 20, Issue 2; ISSN 1463-9262

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

References (44)

Stabilizing Catalytic Pathways via Redundancy: Selective Reduction of Microalgae Oil to Alkanes Peng, Baoxiang; Yuan, Xiaoguo; Zhao, Chen Journal of the American Chemical Society, Vol. 134, Issue 22 https://doi.org/10.1021/ja302436q	journal	May 2012
Converting Algal Triglycerides to Diesel and HEFA Jet Fuel Fractions Robota, Heinz J.; Alger, Jhoanna C.; Shafer, Linda Energy & Fuels, Vol. 27, Issue 2 https://doi.org/10.1021/ef301977b	journal	February 2013
A review of progress in (bio)catalytic routes from/to renewable succinic acid Mazière, Audrey; Prinsen, Pepijn; García, Araceli Biofuels, Bioproducts and Biorefining, Vol. 11, Issue 5 https://doi.org/10.1002/bbb.1785	journal	June 2017
Actinobacillus succinogenes : Advances on succinic acid production and prospects for development of integrated biorefineries Pateraki, Chrysanthi; Patsalou, Maria; Vlysidis, Anestis Biochemical Engineering Journal, Vol. 112 https://doi.org/10.1016/j.bej.2016.04.005	journal	August 2016
Continuous cultivation approach for fermentative succinic acid production from crude glycerol by Basfia succiniciproducens DD1 Scholten, Edzard; Renz, Torsten; Thomas, Jochen Biotechnology Letters, Vol. 31, Issue 12 https://doi.org/10.1007/s10529-009-0104-4	journal	August 2009
Accurate and reliable quantification of total microalgal fuel potential as fatty acid methyl esters by in situ transesterification Laurens, Lieve M. L.; Quinn, Matthew; Van Wychen, Stefanie Analytical and Bioanalytical Chemistry, Vol. 403, Issue 1 https://doi.org/10.1007/s00216-012-5814-0	journal	February 2012
Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli Olajuyin, Ayobami Matthew; Yang, Maohua; Liu, Yilan Bioresource Technology, Vol. 214 https://doi.org/10.1016/j.biortech.2016.04.117	journal	August 2016
Gas-Liquid Equilibria for the System Methane-Butane Nederbragt, G. W. Industrial & Engineering Chemistry, Vol. 30, Issue 5 https://doi.org/10.1021/ie50341a021	journal	May 1938
Biobased chemicals: the convergence of green chemistry with industrial biotechnology Philp, Jim C.; Ritchie, Rachael J.; Allan, Jacqueline E. M. Trends in Biotechnology, Vol. 31, Issue 4 https://doi.org/10.1016/j.tibtech.2012.12.007	journal	April 2013
One-Step Hydrotreatment of Vegetable Oil to Produce High Quality Diesel-Range Alkanes Wang, Congxin; Tian, Zhijian; Wang, Lei ChemSusChem, Vol. 5, Issue 10 https://doi.org/10.1002/cssc.201200219	journal	July 2012
Hydrodeoxygenation of microalgae oil to green diesel over Pt, Rh and presulfided NiMo catalysts Zhou, Lin; Lawal, Adeniyi Catalysis Science & Technology, Vol. 6, Issue 5 https://doi.org/10.1039/C5CY01307K	journal	January 2016
Improvement of hydrothermal stability of Pt/SAPO-11 catalyst in hydrodeoxygenation–isomerization–aromatization of vegetable oil Rabaev, Moshe; Landau, Miron V.; Vidruk-Nehemya, Roxana Journal of Catalysis, Vol. 332 https://doi.org/10.1016/j.jcat.2015.10.005	journal	December 2015
Effect of Cu and Sn promotion on the catalytic deoxygenation of model and algal lipids to fuel-like hydrocarbons over supported Ni catalysts Loe, Ryan; Santillan-Jimenez, Eduardo; Morgan, Tonya Applied Catalysis B: Environmental, Vol. 191 https://doi.org/10.1016/j.apcatb.2016.03.025	journal	August 2016
High quality diesel-range alkanes production via a single-step hydrotreatment of vegetable oil over Ni/zeolite catalyst Wang, Congxin; Liu, Qianhe; Song, Jie Catalysis Today, Vol. 234 https://doi.org/10.1016/j.cattod.2014.02.011	journal	October 2014
Biodiesel and renewable diesel: A comparison Knothe, Gerhard Progress in Energy and Combustion Science, Vol. 36, Issue 3 https://doi.org/10.1016/j.pecs.2009.11.004	journal	June 2010
Palladium-catalyzed decarboxylation of higher aliphatic esters: Towards a new protocol to the second generation biodiesel production Han, Junxing; Sun, Hui; Ding, Yuqi Green Chemistry, Vol. 12, Issue 3 https://doi.org/10.1039/b917690j	journal	January 2010
Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review Dong, Tao; Knoshaug, Eric P.; Pienkos, Philip T. Applied Energy, Vol. 177 https://doi.org/10.1016/j.apenergy.2016.06.002	journal	September 2016
Towards Quantitative Conversion of Microalgae Oil to Diesel-Range Alkanes with Bifunctional Catalysts Peng, Baoxiang; Yao, Yuan; Zhao, Chen Angewandte Chemie, Vol. 124, Issue 9 https://doi.org/10.1002/ange.201106243	journal	November 2011
Catalytic Deoxygenation of Stearic Acid in a Continuous Reactor over a Mesoporous Carbon-Supported Pd Catalyst Lestari, Siswati; Mäki-Arvela, Päivi; Bernas, Heidi Energy & Fuels, Vol. 23, Issue 8 https://doi.org/10.1021/ef900110t	journal	August 2009
Combined algal processing: A novel integrated biorefinery process to produce algal biofuels and bioproducts Dong, Tao; Knoshaug, Eric P.; Davis, Ryan Algal Research, Vol. 19 https://doi.org/10.1016/j.algal.2015.12.021	journal	November 2016
Prospects for a bio-based succinate industry McKinlay, James B.; Vieille, C.; Zeikus, J. Gregory Applied Microbiology and Biotechnology, Vol. 76, Issue 4 https://doi.org/10.1007/s00253-007-1057-y	journal	July 2007
Acid-catalyzed algal biomass pretreatment for integrated lipid and carbohydrate-based biofuels production Laurens, L. M. L.; Nagle, N.; Davis, R. Green Chemistry, Vol. 17, Issue 2 https://doi.org/10.1039/C4GC01612B	journal	January 2015
Significance of CO2 donor on the production of succinic acid by Actinobacillus succinogenes ATCC 55618 Zou, Wei; Zhu, Li-Wen; Li, Hong-Mei Microbial Cell Factories, Vol. 10, Issue 1 https://doi.org/10.1186/1475-2859-10-87	journal	January 2011
Enhanced solid–liquid clarification of lignocellulosic slurries using polyelectrolyte flocculating agents Burke, Devon R.; Anderson, Jason; Gilcrease, Patrick C. Biomass and Bioenergy, Vol. 35, Issue 1 https://doi.org/10.1016/j.biombioe.2010.08.053	journal	January 2011
Laminaria digitata as a potential carbon source for succinic acid and bioenergy production in a biorefinery perspective Alvarado-Morales, Merlin; Gunnarsson, Ingólfur B.; Fotidis, Ioannis A. Algal Research, Vol. 9 https://doi.org/10.1016/j.algal.2015.03.008	journal	May 2015
Biotechnology of succinic acid production and markets for derived industrial products Zeikus, J. G.; Jain, M. K.; Elankovan, P. Applied Microbiology and Biotechnology, Vol. 51, Issue 5 https://doi.org/10.1007/s002530051431	journal	May 1999
Importance of Size and Distribution of Ni Nanoparticles for the Hydrodeoxygenation of Microalgae Oil Song, Wenji; Zhao, Chen; Lercher, Johannes A. Chemistry - A European Journal, Vol. 19, Issue 30 https://doi.org/10.1002/chem.201301005	journal	June 2013
Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen Kuhnert, P.; Scholten, E.; Haefner, S. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 1 https://doi.org/10.1099/ijs.0.011809-0	journal	July 2009
Hydrocarbon Renewable and Synthetic Diesel Fuel Blendstocks: Composition and Properties Smagala, Thomas G.; Christensen, Earl; Christison, Krege M. Energy & Fuels, Vol. 27, Issue 1 https://doi.org/10.1021/ef3012849	journal	November 2012
Hydrocarbons for diesel fuel via decarboxylation of vegetable oils Kubičková, Iva; Snåre, Mathias; Eränen, Kari Catalysis Today, Vol. 106, Issue 1-4 https://doi.org/10.1016/j.cattod.2005.07.188	journal	October 2005
Theoretical Maximum Algal Oil Production Weyer, Kristina M.; Bush, Daniel R.; Darzins, Al BioEnergy Research, Vol. 3, Issue 2 https://doi.org/10.1007/s12155-009-9046-x	journal	October 2009
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances Hu, Qiang; Sommerfeld, Milton; Jarvis, Eric The Plant Journal, Vol. 54, Issue 4 https://doi.org/10.1111/j.1365-313X.2008.03492.x	journal	May 2008
The promise and challenges of microalgal-derived biofuels Pienkos, Philip T.; Darzins, Al Biofuels, Bioproducts and Biorefining, Vol. 3, Issue 4 https://doi.org/10.1002/bbb.159	journal	July 2009
Continuous succinic acid production by Actinobacillus succinogenes on xylose-enriched hydrolysate Bradfield, Michael F. A.; Mohagheghi, Ali; Salvachúa, Davinia Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0363-3	journal	November 2015
The Techno-Economic Basis for Coproduct Manufacturing To Enable Hydrocarbon Fuel Production from Lignocellulosic Biomass Biddy, Mary J.; Davis, Ryan; Humbird, David ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 6 https://doi.org/10.1021/acssuschemeng.6b00243	journal	May 2016
Continuous catalytic deoxygenation of model and algal lipids to fuel-like hydrocarbons over Ni–Al layered double hydroxide Santillan-Jimenez, Eduardo; Morgan, Tonya; Loe, Ryan Catalysis Today, Vol. 258 https://doi.org/10.1016/j.cattod.2014.12.004	journal	December 2015
Investigation the Effect of Oxygenic Compounds on the Isomerization of Bioparaffins Over Pt/SAPO-11 Hancsók, J.; Kovács, S.; Pölczmann, Gy. Topics in Catalysis, Vol. 54, Issue 16-18 https://doi.org/10.1007/s11244-011-9730-x	journal	August 2011
Valorization of macroalga Saccharina latissima as novel feedstock for fermentation-based succinic acid production in a biorefinery approach and economic aspects Marinho, Gonçalo S.; Alvarado-Morales, Merlin; Angelidaki, Irini Algal Research, Vol. 16 https://doi.org/10.1016/j.algal.2016.02.023	journal	June 2016
A low-cost solid–liquid separation process for enzymatically hydrolyzed corn stover slurries Sievers, David A.; Lischeske, James J.; Biddy, Mary J. Bioresource Technology, Vol. 187 https://doi.org/10.1016/j.biortech.2015.03.087	journal	July 2015
Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation Salvachúa, Davinia; Mohagheghi, Ali; Smith, Holly Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0425-1	journal	February 2016
Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen Guettler, M. V.; Rumler, D.; Jain, M. K. International Journal of Systematic Bacteriology, Vol. 49, Issue 1 https://doi.org/10.1099/00207713-49-1-207	journal	January 1999
Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens Salvachúa, Davinia; Smith, Holly; St. John, Peter C. Bioresource Technology, Vol. 214 https://doi.org/10.1016/j.biortech.2016.05.018	journal	August 2016
Solubility of Ethylene in the Presence of Hydrogen in Process Solvents under Polymerization Conditions Sivalingam, G.; Natarajan, V.; Sarma, K. R. Industrial & Engineering Chemistry Research, Vol. 47, Issue 22 https://doi.org/10.1021/ie801236m	journal	November 2008
Heterogeneous-phase equilibrium in ethylene-n-dodecane system Ribeiro, J. Vincent; Susu, Alfred A.; Kohn, James P. Journal of Chemical & Engineering Data, Vol. 17, Issue 3 https://doi.org/10.1021/je60054a032	journal	July 1972

Similar Records

Pretreatment and fermentation of salt-water grown algal biomass as a feedstock for biofuels and high-value biochemicals

Journal Article · 2018 · Algal Research · OSTI ID:1481328

Knoshaug, Eric P.; Dong, Tao; Spiller, Ryan S.; +2 more

Related Subjects

09 BIOMASS FUELS
integrated conversion porcess
algal biomass
feedstocks

Demonstration of Parallel Algal Processing: Production of Renewable Diesel Blendstock and a High-Value Chemical Intermediate

Citation Formats

References (44)

Similar Records

Related Subjects