A compendium of multi-omic sequence information from the Saanich Inlet water column

Hawley, Alyse K.; Torres-Beltrán, Mónica; Zaikova, Elena; Walsh, David A.; Mueller, Andreas; Scofield, Melanie; Kheirandish, Sam; Payne, Chris; Pakhomova, Larysa; Bhatia, Maya; Shevchuk, Olena; Gies, Esther A.; Fairley, Diane; Malfatti, Stephanie A.; Norbeck, Angela D.; Brewer, Heather M.; Pasa-Tolic, Ljiljana; del Rio, Tijana Glavina; Suttle, Curtis A.; Tringe, Susannah; Hallam, Steven J.

doi:10.1038/sdata.2017.160

Title: A compendium of multi-omic sequence information from the Saanich Inlet water column

Journal Article · Tue Oct 31 00:00:00 EDT 2017 · Scientific Data

DOI:https://doi.org/10.1038/sdata.2017.160· OSTI ID:1527025

^[1]; Torres-Beltrán, Mónica ^[1]; Zaikova, Elena ^[2]; Walsh, David A. ^[3]; Mueller, Andreas ^[1]; Scofield, Melanie ^[1]; Kheirandish, Sam ^[1]; Payne, Chris ^[1]; Pakhomova, Larysa ^[1]; Bhatia, Maya ^[1]; Shevchuk, Olena ^[1]; Gies, Esther A. ^[1]; Fairley, Diane ^[1]; Malfatti, Stephanie A. ^[4]; Norbeck, Angela D. ^[5]; Brewer, Heather M. ^[5]; Pasa-Tolic, Ljiljana ^[5]; del Rio, Tijana Glavina ^[4]; Suttle, Curtis A. ^[1]; Tringe, Susannah ^[4] more »

Univ. of British Columbia, Vancouver, BC (Canada)
Georgetown Univ., Washington, DC (United States)
Concordia Univ., Montreal, QC (Canada)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Microbial communities play vital roles in earth’s geochemical cycles. Within marine oxygen minimum zones (OMZs) gradients of oxygen, nitrate and sulfide create redox gradients that drive biogeochemical cycling of carbon, nitrogen and sulphur. Climate-change induced expansion and intensification of OMZs and associated biogeochemical activities has significant implications for green house gas production i.e. nitrous oxide and methane. Next generation sequencing technologies have enabled observations of changes in microbial community structure and expression of RNA and protein along these redox gradients within OMZs. Here, we present a multi-omic time series dataset from Saanich Inlet spanning six years, including high spatial resolution small subunit ribosomal RNA tags, metagenomes, metatranscriptomes, and metaproteomes. As a result, this compendium provides paired multi-omic datasets over multiple time points providing a basis for exploring shifts in microbial community interactions and regulation of metabolic activities both along redox gradients and over time with implications for global climate models.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830

OSTI ID:: 1527025

Alternate ID(s):: OSTI ID: 1416682

Report Number(s):: PNNL-SA-121885

Journal Information:: Scientific Data, Vol. 4; ISSN 2052-4463

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 16 works

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Diverse Marinimicrobia bacteria may mediate coupled biogeochemical cycles along eco-thermodynamic gradients Hawley, Alyse K.; Nobu, Masaru K.; Wright, Jody J. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01376-9	journal	November 2017
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