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Title: Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases

Abstract

The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.

Inventors:
; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1416354
Patent Number(s):
9,862,982
Application Number:
15/164,813
Assignee:
The Regents of the University of California (Oakland, CA); Sandia Corporation (Albuquerque, NM) LBNL
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2016 May 25
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M.. Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases. United States: N. p., 2018. Web.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., & Rubin, Edward M.. Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases. United States.
Zhang, Tao, Datta, Supratim, Simmons, Blake A., and Rubin, Edward M.. Tue . "Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases". United States. doi:. https://www.osti.gov/servlets/purl/1416354.
@article{osti_1416354,
title = {Methods of hydrolyzing a cellulose using halophilic, thermostable and ionic liquids tolerant cellulases},
author = {Zhang, Tao and Datta, Supratim and Simmons, Blake A. and Rubin, Edward M.},
abstractNote = {The present invention provides for an isolated or recombinant polypeptide comprising an amino acid sequence having at least 70% identity with the amino acid sequence of a Halorhabdus utahensis cellulase, such as Hu-CBH1, wherein said amino acid sequence has a halophilic thermostable and/or thermophilic cellobiohydrolase (CBH) activity. In some embodiments, the polypeptide has a CBH activity that is resistant to up to about 20% of ionic liquids. The present invention also provides for compositions comprising and methods using the isolated or recombinant polypeptide.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 09 00:00:00 EST 2018},
month = {Tue Jan 09 00:00:00 EST 2018}
}

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Works referenced in this record:

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

  • Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N.
  • Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384
  • DOI: 10.1016/j.copbio.2005.06.004

Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step
journal, January 2006

  • Dadi, Anantharam P.; Varanasi, Sasidhar; Schall, Constance A.
  • Biotechnology and Bioengineering, Vol. 95, Issue 5, p. 904-910
  • DOI: 10.1002/bit.21047

Molecular simulations provide new insights into the role of the accessory immunoglobulin-like domain of Cel9A
journal, July 2010


Biocatalysis in ionic liquids – advantages beyond green technology
journal, August 2003


Structure of endoglucanase Cel9A from the thermoacidophilic Alicyclobacillus acidocaldarius
journal, July 2009

  • Pereira, Jose Henrique; Sapra, Rajat; Volponi, Joanne V.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 8, p. 744-750
  • DOI: 10.1107/S0907444909012773

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

  • Sen, S.; Venkata Dasu, V.; Mandal, B.
  • Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223
  • DOI: 10.1007/s12010-007-8003-4

Ionic liquid pretreatment of cellulosic biomass: Enzymatic hydrolysis and ionic liquid recycle
journal, December 2010

  • Shill, Kierston; Padmanabhan, Sasisanker; Xin, Qin
  • Biotechnology and Bioengineering, Vol. 108, Issue 3, p. 511-520
  • DOI: 10.1002/bit.23014

Characterization of a metagenome-derived halotolerant cellulase
journal, October 2006


Effect of kosmotropicity of ionic liquids on the enzyme stability in aqueous solutions
journal, February 2006