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Title: Multistage bioassociation of uranium onto an extremely halophilic archaeon revealed by a unique combination of spectroscopic and microscopic techniques

Journal Article · · Journal of Hazardous Materials
 [1];  [1];  [1];  [1];  [2];  [2];  [3];  [3];  [1];  [1]
  1. Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden (Germany). Inst. of Resource Ecology
  2. Helmholtz Centre for Environmental Research - UFZ, Leipzig (Germany). Dept. of Isotope Biogeochemistry
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
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The interactions of two extremely halophilic archaea with uranium were investigated in this paper at high ionic strength as a function of time, pH and uranium concentration. Halobacterium noricense DSM-15987 and Halobacterium sp. putatively noricense, isolated from the Waste Isolation Pilot Plant repository, were used for these investigations. The kinetics of U(VI) bioassociation with both strains showed an atypical multistage behavior, meaning that after an initial phase of U(VI) sorption, an unexpected interim period of U(VI) release was observed, followed by a slow reassociation of uranium with the cells. By applying in situ attenuated total reflection Fourier-transform infrared spectroscopy, the involvement of phosphoryl and carboxylate groups in U(VI) complexation during the first biosorption phase was shown. Differences in cell morphology and uranium localization become visible at different stages of the bioassociation process, as shown with scanning electron microscopy in combination with energy dispersive X-ray spectroscopy. Finally, our results demonstrate for the first time that association of uranium with the extremely halophilic archaeon is a multistage process, beginning with sorption and followed by another process, probably biomineralization.

Research Organization:
Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden (Germany); Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE; Federal Ministry for Economic Affairs and Energy (BMWi) (Germany)
Grant/Contract Number:
AC52-06NA25396; 02E10971
OSTI ID:
1416296
Report Number(s):
LA-UR-17-27411; TRN: US1800904
Journal Information:
Journal of Hazardous Materials, Vol. 327; ISSN 0304-3894
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 27 works
Citation information provided by
Web of Science

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Cited By (3)

Diversity and Niche of Archaea in Bioremediation journal September 2018
Association of Eu(III) and Cm(III) onto an extremely halophilic archaeon journal February 2019
Comparative analysis of uranium bioassociation with halophilic bacteria and archaea journal January 2018

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
58 GEOSCIENCES
uranium bioassociation
halophilic archaeon Halobacterium noricense
in situ ATR FT-IR spectroscopy
luminescence spectroscopy at high salinity
final disposal of radioactive waste