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Title: Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides

Abstract

We describe the co-assembly of two different building units: collagen-mimetic peptides and DNA origami. Two peptides CP++ and sCP(++) are designed with a sequence comprising a central block (Pro-Hyp-Gly) and two positively charged domains (Pro-Arg-Gly) at both N- and C-termini. Co-assembly of peptides and DNA origami two-layer (TL) nanosheets affords the formation of one-dimensional nanowires with repeating periodicity of similar to 10 nm. Structural analyses suggest a face-to-face stacking of DNA nanosheets with peptides aligned perpendicularly to the sheet surfaces. We demonstrate the potential of selective peptide-DNA association between face-to-face and edge-to-edge packing by tailoring the size of DNA nanostructures. This study presents an attractive strategy to create hybrid biomolecular assemblies from peptide and DNA-based building blocks that takes advantage of the intrinsic chemical and physical properties of the respective components to encode structural and, potentially, functional complexity within readily accessible biomimetic materials.

Authors:
 [1];  [2];  [1];  [3];  [1]; ORCiD logo [2]
  1. Department of Chemistry, Emory University, Atlanta, Georgia 30322, United States
  2. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322, United States
  3. X-ray Science Division, Argonne National Laboratory, Argonne, Illinois 60439, United States
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1416014
DOE Contract Number:
AC02-06CH11357
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of the American Chemical Society; Journal Volume: 139; Journal Issue: 40
Country of Publication:
United States
Language:
English
Subject:
arrays; design; model; nanosheets; virus

Citation Formats

Jiang, Tao, Meyer, Travis A., Modlin, Charles, Zuo, Xiaobing, Conticello, Vincent P., and Ke, Yonggang. Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides. United States: N. p., 2017. Web. doi:10.1021/jacs.7b08087.
Jiang, Tao, Meyer, Travis A., Modlin, Charles, Zuo, Xiaobing, Conticello, Vincent P., & Ke, Yonggang. Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides. United States. doi:10.1021/jacs.7b08087.
Jiang, Tao, Meyer, Travis A., Modlin, Charles, Zuo, Xiaobing, Conticello, Vincent P., and Ke, Yonggang. 2017. "Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides". United States. doi:10.1021/jacs.7b08087.
@article{osti_1416014,
title = {Structurally Ordered Nanowire Formation from Co-Assembly of DNA Origami and Collagen-Mimetic Peptides},
author = {Jiang, Tao and Meyer, Travis A. and Modlin, Charles and Zuo, Xiaobing and Conticello, Vincent P. and Ke, Yonggang},
abstractNote = {We describe the co-assembly of two different building units: collagen-mimetic peptides and DNA origami. Two peptides CP++ and sCP(++) are designed with a sequence comprising a central block (Pro-Hyp-Gly) and two positively charged domains (Pro-Arg-Gly) at both N- and C-termini. Co-assembly of peptides and DNA origami two-layer (TL) nanosheets affords the formation of one-dimensional nanowires with repeating periodicity of similar to 10 nm. Structural analyses suggest a face-to-face stacking of DNA nanosheets with peptides aligned perpendicularly to the sheet surfaces. We demonstrate the potential of selective peptide-DNA association between face-to-face and edge-to-edge packing by tailoring the size of DNA nanostructures. This study presents an attractive strategy to create hybrid biomolecular assemblies from peptide and DNA-based building blocks that takes advantage of the intrinsic chemical and physical properties of the respective components to encode structural and, potentially, functional complexity within readily accessible biomimetic materials.},
doi = {10.1021/jacs.7b08087},
journal = {Journal of the American Chemical Society},
number = 40,
volume = 139,
place = {United States},
year = 2017,
month =
}