Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement

Johnson, Chelsea R.; Millwood, Reginald J.; Tang, Yuhong; Gou, Jiqing; Sykes, Robert W.; Turner, Geoffrey B.; Davis, Mark F.; Sang, Yi; Wang, Zeng-Yu; Stewart, Jr., C. Neal

doi:10.1186/s13068-017-0939-1

Title: Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement

Journal Article · Thu Nov 30 00:00:00 EST 2017 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0939-1· OSTI ID:1618700

Johnson, Chelsea R.; Millwood, Reginald J.; Tang, Yuhong; Gou, Jiqing; Sykes, Robert W.; Turner, Geoffrey B.; Davis, Mark F.; Sang, Yi; Wang, Zeng-Yu;

Genetic engineering has been effective in altering cell walls for biofuel production in the bioenergy crop, switchgrass (Panicum virgatum). However, regulatory issues arising from gene flow may prevent commercialization of engineered switchgrass in the eastern United States where the species is native. And, depending on its expression level, microRNA156 (miR156) can reduce, delay, or eliminate flowering, which may serve to decrease transgene flow. Here, in this unique field study of transgenic switchgrass that was permitted to flower, two low (T14 and T35) and two medium (T27 and T37) miR156-overexpressing 'Alamo' lines with the transgene under the control of the constitutive maize (Zea mays) ubiquitin 1 promoter, along with nontransgenic control plants, were grown in eastern Tennessee over two seasons.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Office of Biological and Environmental Research; United States Department of Agriculture (USDA)

Grant/Contract Number:: PS02-06ER64304; AC36-08GO28308

OSTI ID:: 1618700

Alternate ID(s):: OSTI ID: 1414068

Report Number(s):: NREL/JA-2700-70667; 255; PII: 939

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 10 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 9 works

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09 BIOMASS FUELS
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MOESM1 of Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement Johnson, Chelsea; Millwood, Reginald; Tang, Yuhong https://doi.org/10.6084/m9.figshare.5648743.v1 The current record is supplemented by this figure	figure	November 2017
MOESM2 of Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement Johnson, Chelsea; Millwood, Reginald; Tang, Yuhong https://doi.org/10.6084/m9.figshare.5648767.v1 The current record is supplemented by this dataset	dataset	November 2017
Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement [Supplemental Data] Johnson, Chelsea; Millwood, Reginald; Sykes, Robert https://doi.org/10.6084/m9.figshare.c.3942295 The current record is supplemented by this collection	collection	November 2017
Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement [Supplemental Data] Johnson, Chelsea; Millwood, Reginald; Tang, Yuhong https://doi.org/10.6084/m9.figshare.c.3942295.v1 The current record is supplemented by this dataset	dataset	November 2017
MOESM2 of Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement Johnson, Chelsea; Millwood, Reginald; Tang, Yuhong https://doi.org/10.6084/m9.figshare.5648767 The current record is supplemented by this text	text	January 2017