skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A new fluorescence-based method to monitor the pH in the thylakoid lumen using GFP variants

Authors:
; ORCiD logo; ; ;
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1413373
Grant/Contract Number:
FG02-03ER15405
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 486; Journal Issue: 1; Related Information: CHORUS Timestamp: 2017-12-14 11:47:59; Journal ID: ISSN 0006-291X
Publisher:
Elsevier
Country of Publication:
United States
Language:
English

Citation Formats

Yang, Hong, Pu, Xiaojun, Wang, Lu, Liu, Li, and Theg, Steven M. A new fluorescence-based method to monitor the pH in the thylakoid lumen using GFP variants. United States: N. p., 2017. Web. doi:10.1016/j.bbrc.2016.12.032.
Yang, Hong, Pu, Xiaojun, Wang, Lu, Liu, Li, & Theg, Steven M. A new fluorescence-based method to monitor the pH in the thylakoid lumen using GFP variants. United States. doi:10.1016/j.bbrc.2016.12.032.
Yang, Hong, Pu, Xiaojun, Wang, Lu, Liu, Li, and Theg, Steven M. Sat . "A new fluorescence-based method to monitor the pH in the thylakoid lumen using GFP variants". United States. doi:10.1016/j.bbrc.2016.12.032.
@article{osti_1413373,
title = {A new fluorescence-based method to monitor the pH in the thylakoid lumen using GFP variants},
author = {Yang, Hong and Pu, Xiaojun and Wang, Lu and Liu, Li and Theg, Steven M.},
abstractNote = {},
doi = {10.1016/j.bbrc.2016.12.032},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 486,
place = {United States},
year = {Sat Apr 01 00:00:00 EDT 2017},
month = {Sat Apr 01 00:00:00 EDT 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.bbrc.2016.12.032

Save / Share:
  • At2g44920 belongs to a diverse family (Pfam PF00805) of pentapeptide-repeat proteins (PRPs) that are present in all known organisms except yeast. PRPs contain at least eight tandem-repeating sequences of five amino acids with an approximate consensus sequence (STAV)(D/N)(L/F)(S/T/R)(X). Recent crystal structures show that PRPs adopt a highly regular four-sided right-handed {beta}-helical structure consisting mainly of type II and type IV {beta}-turns, sometimes referred to as a repeated five-residue (or Rfr) fold. Among sequenced genomes, PRP genes are most abundant in cyanobacteria, leading to speculation that PRPs play an important role in the unique lifestyle of photosynthetic cyanobacteria. Despite the recentmore » structural characterization of several cyanobacterial PRPs, most of their functions remain unknown. Plants, whose chloroplasts are of cyanobacterial origin, have only four PRP genes in their genomes. At2g44920 is one of three PRPs located in the thylakoid lumen. Here, the crystal structure of a double methionine mutant of residues 81-224 of At2g44920, the naturally processed fragment of one of its full-length isoforms, is reported at 1.7 {angstrom} resolution. The structure of At2g44920 consists of the characteristic Rfr fold with five uninterrupted coils made up of 25 pentapeptide repeats and {alpha}-helical elements capping both termini. A disulfide bridge links the two {alpha}-helices with a conserved loop between the helical elements at its C-terminus. This structure represents the first structure of a PRP protein whose subcellular location has been experimentally confirmed to be the thylakoid lumen in a plant species.« less
  • No abstract prepared.
  • A technique for measuring ''effective'' absorption coefficients is described. It circumvents deviations from Beer's law caused when the excitation source bandwidth is larger than the absorber bandwidth. The technique employs a fluorescence cell placed after an absorption cell to selectively monitor absorption in the center region of the source line. Model calculations relating the fluorescence intensity to source and absorber line shapes indicate that this method should yield linear Beer's law plots for moderate values of k/sub 0/Nl and ..cap alpha.., where ..cap alpha.. is the ratio of the source bandwidth to the absorber bandwidth. This technique has been appliedmore » to a number of single rotational levels in the 4/sup 1//sub 0/ transition of the H/sub 2/CO A /sup 1/A/sub 2/reverse arrowX /sup 1/A/sub 1/ system using pulsed, tunable dye laser excitation. The effective absorption coefficients determined experimentally have been compared to the theoretically calculated absorption coefficients.« less
  • Iridium 192 seeds contained in a ribbon were preloaded into a new double lumen 11 Fr endoprosthesis which was then inserted into malignant strictures of the bile duct and ampulla and left in place for 48 hours until 5000 rads were delivered to the tumor. The procedure was carried out in 14 patients (7 women, 7 men; mean age, 63.2 years; range, 46 to 86 years). Six patients were treated for cholangiocarcinomas, four with pancreatic carcinomas, and four with ampullary carcinomas. No complications occurred. The mean survival of the group was 7 months (range, 3 days to 27 months). Thismore » new technique provides both intraluminal brachytherapy and biliary drainage and is inserted intraduodenally across the papilla of Vater avoiding puncture of the liver and external hardware required by the percutaneous technique and hardware necessitated with a nasobiliary tube. Following removal of the iridium prosthesis, a large caliber endoprosthesis is inserted for continued decompression. Because of proven efficacy of endoprostheses, this new technique should be considered when intraluminal irradiation is indicated.« less