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Title: Identification of bottlenecks in the accumulation of cyclic fatty acids in camelina seed oil

Journal Article · · Plant Biotechnology Journal
DOI:https://doi.org/10.1111/pbi.12839· OSTI ID:1412647
 [1];  [2];  [3];  [4];  [1];  [5];  [4];  [3];  [2];  [6];  [6]
  1. Stony Brook Univ., NY (United States). Dept. of Chemistry. Biochemistry and Cell Biology Dept.
  2. Univ. of Nebrask. Center for Plant Science Innovation and Dept. of Biochemistry
  3. Univ. of North Texas, Denton, TX (United States). Center for Advanced Scientific Computing and Modeling (CASCaM). Dept. of Biological Sciences
  4. Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.
  5. Stony Brook Univ., NY (United States). Biochemistry and Cell Biology Dept.
  6. Stony Brook Univ., NY (United States). Dept. of Chemistry. Biochemistry and Cell Biology Dept.; Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.
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Modified fatty acids (mFA) have diverse uses, e.g., cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics, and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring source plants. In order to further our understanding of metabolic bottlenecks that limit mFA accumulation, we generated transgenic Camelina sativa lines co-expressing Escherichia coli cyclopropane synthase (EcCPS) and Sterculia foetida lysophosphatidic acid acyltransferase (SfLPAT). In contrast to transgenic CPA-accumulating Arabidopsis, CPA accumulation in camelina caused only minor changes in seed weight, germination rate, oil accumulation, and seedling development. CPA accumulated to much higher levels in membrane than storage lipids, comprising more than 60% of total fatty acid in both phosphatidylcholine (PC) and phosphatidylethanolamine (PE) versus 26% in diacylglycerol (DAG) and 12% in triacylglycerol (TAG) indicating bottlenecks in the transfer of CPA from PC to DAG and from DAG to TAG. Upon coexpression of SfLPAT with EcCPS, di-CPA-PC increased by ~50% relative to lines expressing EcCPS alone with the di-CPA-PC primarily observed in the embryonic axis and mono-CPA-PC primarily in cotyledon tissue. EcCPS-SfLPAT lines revealed a redistribution of CPA from the sn-1 to sn-2 positions within PC and PE that was associated with a doubling of CPA accumulation in both DAG and TAG. Finally, the identification of metabolic bottlenecks in acyl transfer between site of synthesis (phospholipids) and deposition in storage oils (TAGs) lays the foundation for the optimizing CPA accumulation through directed engineering of oil synthesis in target crops.

Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
Grant/Contract Number:
SC0012704; FG02-09ER64812; KC0304000; DBI 1117680
OSTI ID:
1412647
Report Number(s):
BNL-114333-2017-JA
Journal Information:
Plant Biotechnology Journal, Vol. 16, Issue 4; ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied BiologyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 25 works
Citation information provided by
Web of Science

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Cited By (4)

Tissue-specific differences in metabolites and transcripts contribute to the heterogeneity of ricinoleic acid accumulation in Ricinus communis L. (castor) seeds journal January 2019
Cyclopropane fatty acid biosynthesis in plants: phylogenetic and biochemical analysis of Litchi Kennedy pathway and acyl editing cycle genes journal August 2018
Enhanced Production of High‐Value Cyclopropane Fatty Acid in Yeast Engineered for Increased Lipid Synthesis and Accumulation journal October 2018
Producing Cyclopropane Fatty Acid in Plant Leafy Biomass via Expression of Bacterial and Plant Cyclopropane Fatty Acid Synthases journal February 2020

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
cyclopropane fatty acid
Camelina sativa
unusual fatty acid
lipid metabolism
triacylglycerol
lipid synthesis