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Title: Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

Abstract

The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with an allotetraploid genome (2n = 4x = 36), a complexity which forms an impediment to generating homozygous knock-out plants. Lignin, a major component of the plant cell wall and a contributor to cellulosic feedstock’s recalcitrance to decomposition, stands as a barrier to efficient biofuel production by limiting enzyme access to cell wall polymers during the fermentation process.

Authors:
 [1];  [2];  [1];  [2];  [3];  [3];  [4]; ORCiD logo [1]
  1. Noble Research Inst., Ardmore, OK (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Noble Research Inst., Ardmore, OK (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1412035
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Genome editing; CRISPR/Cas9; Switchgrass; Panicum virgatum; Bioenergy; Lignin biosynthesis; 4-Coumarate:coenzyme A ligase; 4CL; Sugar release

Citation Formats

Park, Jong -Jin, Yoo, Chang Geun, Flanagan, Amy, Pu, Yunqiao, Debnath, Smriti, Ge, Yaxin, Ragauskas, Arthur J., and Wang, Zeng -Yu. Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0972-0.
Park, Jong -Jin, Yoo, Chang Geun, Flanagan, Amy, Pu, Yunqiao, Debnath, Smriti, Ge, Yaxin, Ragauskas, Arthur J., & Wang, Zeng -Yu. Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release. United States. doi:10.1186/s13068-017-0972-0.
Park, Jong -Jin, Yoo, Chang Geun, Flanagan, Amy, Pu, Yunqiao, Debnath, Smriti, Ge, Yaxin, Ragauskas, Arthur J., and Wang, Zeng -Yu. Thu . "Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release". United States. doi:10.1186/s13068-017-0972-0. https://www.osti.gov/servlets/purl/1412035.
@article{osti_1412035,
title = {Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release},
author = {Park, Jong -Jin and Yoo, Chang Geun and Flanagan, Amy and Pu, Yunqiao and Debnath, Smriti and Ge, Yaxin and Ragauskas, Arthur J. and Wang, Zeng -Yu},
abstractNote = {The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with an allotetraploid genome (2n = 4x = 36), a complexity which forms an impediment to generating homozygous knock-out plants. Lignin, a major component of the plant cell wall and a contributor to cellulosic feedstock’s recalcitrance to decomposition, stands as a barrier to efficient biofuel production by limiting enzyme access to cell wall polymers during the fermentation process.},
doi = {10.1186/s13068-017-0972-0},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = 1,
volume = 10,
place = {United States},
year = {2017},
month = {11}
}

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