Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

Park, Jong-Jin; Yoo, Chang Geun; Flanagan, Amy; Pu, Yunqiao; Debnath, Smriti; Ge, Yaxin; Ragauskas, Arthur J.; Wang, Zeng-Yu

doi:10.1186/s13068-017-0972-0

Title: Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

Journal Article · Thu Nov 30 00:00:00 EST 2017 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0972-0· OSTI ID:1618709

Park, Jong-Jin; Yoo, Chang Geun; Flanagan, Amy; Pu, Yunqiao; Debnath, Smriti; Ge, Yaxin; Ragauskas, Arthur J.;

The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with an allotetraploid genome (2n = 4x = 36), a complexity which forms an impediment to generating homozygous knock-out plants. Lignin, a major component of the plant cell wall and a contributor to cellulosic feedstock’s recalcitrance to decomposition, stands as a barrier to efficient biofuel production by limiting enzyme access to cell wall polymers during the fermentation process.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1618709

Alternate ID(s):: OSTI ID: 1412035

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 10 Journal Issue: 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 39 works

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References (33)

Enabling plant synthetic biology through genome engineering Baltes, Nicholas J.; Voytas, Daniel F. Trends in Biotechnology, Vol. 33, Issue 2 https://doi.org/10.1016/j.tibtech.2014.11.008	journal	February 2015
An Improved Method for Clearing and Staining Free-hand Sections and Whole-mount Samples* Lux, Alexander; Morita, Shigenori; Abe, Jun Annals of Botany, Vol. 96, Issue 6 https://doi.org/10.1093/aob/mci266	journal	September 2005
Two-year field analysis of reduced recalcitrance transgenic switchgrass Baxter, Holly L.; Mazarei, Mitra; Labbe, Nicole Plant Biotechnology Journal, Vol. 12, Issue 7 https://doi.org/10.1111/pbi.12195	journal	April 2014
Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system Belhaj, Khaoula; Chaparro-Garcia, Angela; Kamoun, Sophien Plant Methods, Vol. 9, Issue 1 https://doi.org/10.1186/1746-4811-9-39	journal	January 2013
Antisense suppression of 4-coumarate:coenzyme A ligase activity in Arabidopsis leads to altered lignin subunit composition. Lee, D.; Meyer, K.; Chapple, C. The Plant Cell, Vol. 9, Issue 11 https://doi.org/10.1105/tpc.9.11.1985	journal	November 1997
Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808 https://doi.org/10.1073/pnas.1100310108	journal	February 2011
Lignin modification improves fermentable sugar yields for biofuel production Chen, Fang; Dixon, Richard A. Nature Biotechnology, Vol. 25, Issue 7, p. 759-761 https://doi.org/10.1038/nbt1316	journal	June 2007
Simultaneous Downregulation of MTHFR and COMT in Switchgrass Affects Plant Performance and Induces Lesion-Mimic Cell Death Liu, Sijia; Fu, Chunxiang; Gou, Jiqing Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.00982	journal	June 2017
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering Gaj, Thomas; Gersbach, Charles A.; Barbas, Carlos F. Trends in Biotechnology, Vol. 31, Issue 7, p. 397-405 https://doi.org/10.1016/j.tibtech.2013.04.004	journal	July 2013
Is genetic engineering ever going to take off in forage, turf and bioenergy crop breeding? Wang, Zeng-Yu; Brummer, E. Charles Annals of Botany, Vol. 110, Issue 6 https://doi.org/10.1093/aob/mcs027	journal	February 2012
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems DiCarlo, James E.; Norville, Julie E.; Mali, Prashant Nucleic Acids Research, Vol. 41, Issue 7, p. 4336-4343 https://doi.org/10.1093/nar/gkt135	journal	March 2013
Describing and Quantifying Growth Stages of Perennial Forage Grasses Moore, K. J.; Moser, L. E.; Vogel, K. P. Agronomy Journal, Vol. 83, Issue 6 https://doi.org/10.2134/agronj1991.00021962008300060027x	journal	January 1991
New insights into the molecular architecture of hardwood lignins by chemical degradative methods Lapierre, Catherine; Pollet, Brigitte; Rolando, Christian Research on Chemical Intermediates, Vol. 21, Issue 3-5, p. 397-412 https://doi.org/10.1007/BF03052266	journal	March 1995
Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation Mann, David GJ; King, Zachary R.; Liu, Wusheng BMC Biotechnology, Vol. 11, Issue 1 https://doi.org/10.1186/1472-6750-11-74	journal	July 2011
Gene-edited CRISPR mushroom escapes US regulation Waltz, Emily Nature, Vol. 532, Issue 7599 https://doi.org/10.1038/nature.2016.19754	journal	April 2016
Pollen Viability and Longevity of Switchgrass ( L.) Ge, Yaxin; Fu, Chunxiang; Bhandari, Hem Crop Science, Vol. 51, Issue 6 https://doi.org/10.2135/cropsci2011.01.0057	journal	January 2011
Standardization of Switchgrass Sample Collection for Cell Wall and Biomass Trait Analysis Hardin, C. Frank; Fu, Chunxiang; Hisano, Hiroshi BioEnergy Research, Vol. 6, Issue 2 https://doi.org/10.1007/s12155-012-9292-1	journal	January 2013
The substrate specificity-determining amino acid code of 4-coumarate:CoA ligase Schneider, Katja; Hövel, Klaus; Witzel, Kilian Proceedings of the National Academy of Sciences, Vol. 100, Issue 14 https://doi.org/10.1073/pnas.1430550100	journal	June 2003
Complete Switchgrass Genetic Maps Reveal Subgenome Collinearity, Preferential Pairing and Multilocus Interactions Okada, Miki; Lanzatella, Christina; Saha, Malay C. Genetics, Vol. 185, Issue 3 https://doi.org/10.1534/genetics.110.113910	journal	April 2010
Regulating transgenic crops sensibly: lessons from plant breeding, biotechnology and genomics Bradford, Kent J.; Van Deynze, Allen; Gutterson, Neal Nature Biotechnology, Vol. 23, Issue 4 https://doi.org/10.1038/nbt1084	journal	April 2005
Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production Xu, Bin; Escamilla-Treviño, Luis L.; Sathitsuksanoh, Noppadon New Phytologist, Vol. 192, Issue 3 https://doi.org/10.1111/j.1469-8137.2011.03830.x	journal	July 2011
Using the Acetyl Bromide Assay To Determine Lignin Concentrations in Herbaceous Plants: Some Cautionary Notes Hatfield, R. D.; Grabber, J.; Ralph, J. Journal of Agricultural and Food Chemistry, Vol. 47, Issue 2, p. 628-632 https://doi.org/10.1021/jf9808776	journal	February 1999
Downregulation of Cinnamyl Alcohol Dehydrogenase (CAD) Leads to Improved Saccharification Efficiency in Switchgrass Fu, Chunxiang; Xiao, Xirong; Xi, Yajun BioEnergy Research, Vol. 4, Issue 3, p. 153-164 https://doi.org/10.1007/s12155-010-9109-z	journal	January 2011
The miR156 - SPL4 module predominantly regulates aerial axillary bud formation and controls shoot architecture Gou, Jiqing; Fu, Chunxiang; Liu, Sijia New Phytologist, Vol. 216, Issue 3 https://doi.org/10.1111/nph.14758	journal	September 2017
Homeologous Recombination Plays a Major Role in Chromosome Rearrangements That Occur During Meiosis of Brassica napus Haploids Nicolas, Stéphane D.; Mignon, Guillaume Le; Eber, Frédérique Genetics, Vol. 175, Issue 2 https://doi.org/10.1534/genetics.106.062968	journal	December 2006
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease Cho, Seung Woo; Kim, Sojung; Kim, Jong Min Nature Biotechnology, Vol. 31, Issue 3 https://doi.org/10.1038/nbt.2507	journal	January 2013
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew Wang, Yanpeng; Cheng, Xi; Shan, Qiwei Nature Biotechnology, Vol. 32, Issue 9 https://doi.org/10.1038/nbt.2969	journal	July 2014
A Genomics Approach to Deciphering Lignin Biosynthesis in Switchgrass Shen, H.; Mazarei, M.; Hisano, H. The Plant Cell, Vol. 25, Issue 11 https://doi.org/10.1105/tpc.113.118828	journal	November 2013
Cellulosic Biofuels Carroll, Andrew; Somerville, Chris Annual Review of Plant Biology, Vol. 60, Issue 1 https://doi.org/10.1146/annurev.arplant.043008.092125	journal	June 2009
The CRISPR/Cas9 system for plant genome editing and beyond Bortesi, Luisa; Fischer, Rainer Biotechnology Advances, Vol. 33, Issue 1 https://doi.org/10.1016/j.biotechadv.2014.12.006	journal	January 2015
Switchgrass SBP-box transcription factors PvSPL1 and 2 function redundantly to initiate side tillers and affect biomass yield of energy crop Wu, Zhenying; Cao, Yingping; Yang, Ruijuan Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0516-z	journal	May 2016
Transgenic switchgrass ( Panicum virgatum L.) targeted for reduced recalcitrance to bioconversion: a 2-year comparative analysis of field-grown lines modified for target gene or genetic element expression Dumitrache, Alexandru; Natzke, Jace; Rodriguez, Miguel Plant Biotechnology Journal, Vol. 15, Issue 6 https://doi.org/10.1111/pbi.12666	journal	February 2017
Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system Xie, Kabin; Minkenberg, Bastian; Yang, Yinong Proceedings of the National Academy of Sciences, Vol. 112, Issue 11 https://doi.org/10.1073/pnas.1420294112	journal	March 2015

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09 BIOMASS FUELS
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Title: Defined tetra-allelic gene disruption of the 4-coumarate:coenzyme A ligase 1 (Pv4CL1) gene by CRISPR/Cas9 in switchgrass results in lignin reduction and improved sugar release

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