skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems

Abstract

Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from natural variants are not clear. In addition, the sugar release patterns resulting from digestion with two distinct biological systems, fungal enzymes and Clostridium thermocellum, have yet to be evaluated and compared. This study evaluates the effect of structural features of three natural variant Populus lines, which includes the line BESC standard, with respect to the overall process of sugar release for two different biological systems.

Authors:
 [1];  [1];  [1];  [2];  [3];  [3];  [3];  [4];  [5];  [6];  [1];  [1]
  1. Univ. of California, Riverside, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Georgia Inst. of Technology, Atlanta, GA (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States); Mascoma LLC, Lebanon, NH (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States)
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1412034
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Populus; Natural variants; Hydrothermal pretreatment; Structural changes; Enzymatic hydrolysis; Consolidated bioprocessing; Clostridium thermocellum

Citation Formats

Thomas, Vanessa A., Kothari, Ninad, Bhagia, Samarthya, Akinosho, Hannah, Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Pattathil, Sivakumar, Hahn, Michael G., Raguaskas, Arthur J., Wyman, Charles E., and Kumar, Rajeev. Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems. United States: N. p., 2017. Web. doi:10.1186/s13068-017-0975-x.
Thomas, Vanessa A., Kothari, Ninad, Bhagia, Samarthya, Akinosho, Hannah, Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Pattathil, Sivakumar, Hahn, Michael G., Raguaskas, Arthur J., Wyman, Charles E., & Kumar, Rajeev. Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems. United States. doi:10.1186/s13068-017-0975-x.
Thomas, Vanessa A., Kothari, Ninad, Bhagia, Samarthya, Akinosho, Hannah, Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Pattathil, Sivakumar, Hahn, Michael G., Raguaskas, Arthur J., Wyman, Charles E., and Kumar, Rajeev. Thu . "Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems". United States. doi:10.1186/s13068-017-0975-x. https://www.osti.gov/servlets/purl/1412034.
@article{osti_1412034,
title = {Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems},
author = {Thomas, Vanessa A. and Kothari, Ninad and Bhagia, Samarthya and Akinosho, Hannah and Li, Mi and Pu, Yunqiao and Yoo, Chang Geun and Pattathil, Sivakumar and Hahn, Michael G. and Raguaskas, Arthur J. and Wyman, Charles E. and Kumar, Rajeev},
abstractNote = {Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from natural variants are not clear. In addition, the sugar release patterns resulting from digestion with two distinct biological systems, fungal enzymes and Clostridium thermocellum, have yet to be evaluated and compared. This study evaluates the effect of structural features of three natural variant Populus lines, which includes the line BESC standard, with respect to the overall process of sugar release for two different biological systems.},
doi = {10.1186/s13068-017-0975-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {United States},
year = {Thu Nov 30 00:00:00 EST 2017},
month = {Thu Nov 30 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Dilute Acid and Autohydrolysis Pretreatment
book, August 2009


Small-scale and automatable high-throughput compositional analysis of biomass
journal, October 2010

  • DeMartini, Jaclyn D.; Studer, Michael H.; Wyman, Charles E.
  • Biotechnology and Bioengineering, Vol. 108, Issue 2, p. 306-312
  • DOI: 10.1002/bit.22937

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

The impact of dilute sulfuric acid on the selectivity of xylooligomer depolymerization to monomers
journal, February 2008


Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii
journal, June 2014

  • Chung, D.; Cha, M.; Guss, A. M.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 24, p. 8931-8936
  • DOI: 10.1073/pnas.1402210111

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010

  • Pattathil, S.; Avci, U.; Baldwin, D.
  • Plant Physiology, Vol. 153, Issue 2, p. 514-525
  • DOI: 10.1104/pp.109.151985

Recent progress in consolidated bioprocessing
journal, June 2012

  • Olson, Daniel G.; McBride, John E.; Joe Shaw, A.
  • Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405
  • DOI: 10.1016/j.copbio.2011.11.026

Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids
journal, December 2005