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Title: Development of reactor configurations for an electrofuels platform utilizing genetically modified iron oxidizing bacteria for the reduction of CO 2 to biochemicals

Authors:
; ; ; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1412023
Grant/Contract Number:
AR0000553; 38309
Resource Type:
Journal Article: Publisher's Accepted Manuscript
Journal Name:
Journal of Biotechnology
Additional Journal Information:
Journal Volume: 245; Journal Issue: C; Related Information: CHORUS Timestamp: 2017-12-08 16:55:02; Journal ID: ISSN 0168-1656
Publisher:
Elsevier
Country of Publication:
Netherlands
Language:
English

Citation Formats

Guan, Jingyang, Berlinger, Sarah A., Li, Xiaozheng, Chao, Zhongmou, Sousa e Silva, Victor, Banta, Scott, and West, Alan C. Development of reactor configurations for an electrofuels platform utilizing genetically modified iron oxidizing bacteria for the reduction of CO 2 to biochemicals. Netherlands: N. p., 2017. Web. doi:10.1016/j.jbiotec.2017.02.004.
Guan, Jingyang, Berlinger, Sarah A., Li, Xiaozheng, Chao, Zhongmou, Sousa e Silva, Victor, Banta, Scott, & West, Alan C. Development of reactor configurations for an electrofuels platform utilizing genetically modified iron oxidizing bacteria for the reduction of CO 2 to biochemicals. Netherlands. doi:10.1016/j.jbiotec.2017.02.004.
Guan, Jingyang, Berlinger, Sarah A., Li, Xiaozheng, Chao, Zhongmou, Sousa e Silva, Victor, Banta, Scott, and West, Alan C. Wed . "Development of reactor configurations for an electrofuels platform utilizing genetically modified iron oxidizing bacteria for the reduction of CO 2 to biochemicals". Netherlands. doi:10.1016/j.jbiotec.2017.02.004.
@article{osti_1412023,
title = {Development of reactor configurations for an electrofuels platform utilizing genetically modified iron oxidizing bacteria for the reduction of CO 2 to biochemicals},
author = {Guan, Jingyang and Berlinger, Sarah A. and Li, Xiaozheng and Chao, Zhongmou and Sousa e Silva, Victor and Banta, Scott and West, Alan C.},
abstractNote = {},
doi = {10.1016/j.jbiotec.2017.02.004},
journal = {Journal of Biotechnology},
number = C,
volume = 245,
place = {Netherlands},
year = {Wed Mar 01 00:00:00 EST 2017},
month = {Wed Mar 01 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.jbiotec.2017.02.004

Citation Metrics:
Cited by: 2works
Citation information provided by
Web of Science

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  • Acidophilic bacteria of the genera Thiobacillus and Sulfolobus are able to reduce ferric iron when growing on elemental sulfur as an energy source. It has been previously thought that ferric iron serves as a nonbiological oxidant in the formation of acid mine drainage and in the leaching of ores, but these results suggest that bacterial catalysis may play a significant role in the reactivity of ferric iron.
  • An electrochemical apparatus for culturing chemolithotrophic bacteria that respire aerobically on ferrous ions is described. Enhanced yields of the bacteria were achieved by the in situ electrochemical reduction of soluble iron in the growth medium. When subjected to a direct current of 30 A for 60 days, a 45-liter culture of Thiobacillus ferrooxidans grew from 6 x 10{sup 7} to 9.5 x 10{sup 9} cells per ml. Growth of the bacterium within the electrolytic bioreactor was linear with time. A final cell density corresponding to 4.7 g of wet cell paste per liter was achieved, and a total of 320more » g of wet cell paste was harvested from one culture. The apparatus was designed to deliver protons concomitantly with electrons; therefore, the pH of the culture remained stable at 1.6 {+-} 0.1 for the duration of growth. This laboratory-scale apparatus may be readily adapted to pilot or production scale. It is thus anticipated that abundant numbers of iron-oxidizing bacteria may be obtained for both fundamental and applied studies. 35 refs., 6 figs.« less
  • Electrofuels Project: Penn State is genetically engineering bacteria called Rhodobacter to use electricity or electrically generated hydrogen to convert carbon dioxide into liquid fuels. Penn State is taking genes from oil-producing algae called Botryococcus braunii and putting them into Rhodobacter to produce hydrocarbon molecules, which closely resemble gasoline. Penn State is developing engineered tanks to support microbial fuel production and determining the most economical way to feed the electricity or hydrogen to the bacteria, including using renewable sources of power like solar energy.
  • The quantitative contribution of fatty acids and CO/sub 2/ to methanogenesis was studied by using stirred, 3-liter bench-top digestors fed on a semicontinuous basis with cattle waste. The fermentations were carried out at 40 and 60/sup 0/C under identical loading conditions. In the thermophilic digestor, acetate turnover increased from a prefeeding level of 16 ..mu..M/min to a peak (49 ..mu..M/min. Acetate turnover in the mesophilic digester increased fron 15 to 40 ..mu..M/min. Propionate turnover ranged from 2 to 5.2 and 1.5 to 4.5 ..mu..M/min in the thermophilic and mesophilic digestors, respectively. Butyrate turnover (0.7 to 1.2 ..mu..M/min) was similar inmore » both digestors. The proportion of CH/sub 4/ produced via the methyl group of acetate varied with time after feeding and ranged from 72 to 75% in the mesophilic digestor and 75 to 86% in the thermophilic digestor. The contribution from CO/sub 2/ reduction was 24 to 19% and 19 to 27%, respectively. Propionate and butyrate turnover accounted for 20% of the total CH/sub 4/ produced. Counts of fatty acid-degrading bacteria were related to their turnover activity.« less
  • As researchers engineer cyanobacteria for biotechnological applications, we must consider potential environmental release of these organisms. Previous theoretical work has considered cyanobacterial containment through elimination of the CO 2-concentrating mechanism (CCM) to impose a high-CO 2 requirement (HCR), which could be provided in the cultivation environment but not in the surroundings. In this work, we experimentally implemented an HCR containment mechanism in Synechococcus sp. strain PCC7002 (PCC7002) through deletion of carboxysome shell proteins and showed that this mechanism contained cyanobacteria in a 5% CO 2 environment. We considered escape through horizontal gene transfer (HGT) and reduced the risk of HGTmore » escape by deleting competence genes. We showed that the HCR containment mechanism did not negatively impact the performance of a strain of PCC7002 engineered for L-lactate production. In conclusion, we showed through coculture experiments of HCR strains with ccm-containing strains that this HCR mechanism reduced the frequency of escape below the NIH recommended limit for recombinant organisms of one escape event in 10 8 CFU.« less