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Title: Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses

Journal Article · · Plant Physiology (Bethesda)
DOI:https://doi.org/10.1104/pp.15.00575· OSTI ID:1411727
 [1];  [1]; ORCiD logo [2];  [3];  [4];  [5];  [6]; ORCiD logo [7];  [8]; ORCiD logo [9];  [10];  [4]; ORCiD logo [11];  [12]
  1. Univ. of Heidelberg (Germany). Centre for Organismal Studies; Univ. of California, San Diego, CA (United States)
  2. Univ. of California, San Diego, CA (United States); Scripps Research Inst., La Jolla, CA (United States). Dept. of Chemical Physiology
  3. Okayama Univ. (Japan); Univ. of California, San Diego, CA (United States)
  4. Brown Univ., Providence, RI (United States). Dept. of Molecular Biology Cell Biology and Biochemistry
  5. Scripps Research Inst., La Jolla, CA (United States). Dept. of Chemical Physiology; Univ. of California, San Diego, CA (United States)
  6. Okayama Univ. (Japan)
  7. Donald Danforth Plant Science Center, St. Louis, MS (United States). Dept. of Biology
  8. Univ. of Southern California, Los Angeles, CA (United States)
  9. Brown Univ., Providence, RI (United States). Dept. of Molecular Biology Cell Biology and Biochemistry; Univ. of California, San Diego, CA (United States)
  10. Univ. of Heidelberg (Germany). Centre for Organismal Studies
  11. Scripps Research Inst., La Jolla, CA (United States). Dept. of Chemical Physiology
  12. Univ. of Southern California, Los Angeles, CA (United States); Univ. of California, San Diego, CA (United States)
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The plant hormone abscisic acid (ABA) controls growth and development and regulates plant water status through an established signaling pathway. In the presence of ABA, pyrabactin resistance/regulatory component of ABA receptor proteins inhibit type 2C protein phosphatases (PP2Cs). This, in turn, enables the activation of Sucrose Nonfermenting1-Related Protein Kinases2 (SnRK2). Open Stomata1 (OST1)/SnRK2.6/SRK2E is a major SnRK2-type protein kinase responsible for mediating ABA responses. Arabidopsis (Arabidopsis thaliana) expressing an epitope-tagged OST1 in the recessive ost1-3 mutant background was used for the copurification and identification of OST1-interacting proteins after osmotic stress and ABA treatments. Furthemore, these analyses, which were confirmed using bimolecular fluorescence complementation and coimmunoprecipitation, unexpectedly revealed homo- and heteromerization of OST1 with SnRK2.2, SnRK2.3, OST1, and SnRK2.8. Furthermore, several OST1-complexed proteins were identified as type 2A protein phosphatase (PP2A) subunits and as proteins involved in lipid and galactolipid metabolism. More detailed analyses suggested an interaction network between ABA-activated SnRK2-type protein kinases and several PP2A-type protein phosphatase regulatory subunits. pp2a double mutants exhibited a reduced sensitivity to ABA during seed germination and stomatal closure and an enhanced ABA sensitivity in root growth regulation. Our analyses add PP2A-type protein phosphatases as another class of protein phosphatases to the interaction network of SnRK2-type protein kinases.

Research Organization:
Univ. of California, San Diego, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
FG02-03ER15449
OSTI ID:
1411727
Journal Information:
Plant Physiology (Bethesda), Vol. 169, Issue 1; ISSN 0032-0889
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 65 works
Citation information provided by
Web of Science

References (1)

Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses journal July 2015

Cited By (21)

Root Growth Adaptation is Mediated by PYLs ABA Receptor‐PP2A Protein Phosphatase Complex journal December 2019
Precise control of ABA signaling through post-translational protein modification journal April 2019
Identification and characterization of core abscisic acid (ABA) signaling components and their gene expression profile in response to abiotic stresses in Setaria viridis journal March 2019
Abscisic acid-independent stomatal CO 2 signal transduction pathway and convergence of CO 2 and ABA signaling downstream of OST1 kinase journal October 2018
Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity journal October 2016
The SAUR41 subfamily of cell expansion-promoting genes modulates abscisic acid sensitivity and root touch response: a possible connection to ion homeostasis regulation journal December 2019
Spatio-temporal aspects of Ca2+ signalling: lessons from guard cells and pollen tubes journal April 2018
An Apple Protein Kinase MdSnRK1.1 Interacts with MdCAIP1 to Regulate ABA Sensitivity journal July 2017
Ethylene Inhibits Methyl Jasmonate-Induced Stomatal Closure by Modulating Guard Cell Slow-Type Anion Channel Activity via the OPEN STOMATA 1/SnRK2.6 Kinase-Independent Pathway in Arabidopsis journal June 2019
The Sucrose Non-Fermenting 1-Related Protein Kinase 2 (SnRK2) Genes Are Multifaceted Players in Plant Growth, Development and Response to Environmental Stimuli journal December 2019
Post-translational control of ABA signalling: the roles of protein phosphorylation and ubiquitination journal December 2016
Protein phosphatase 2A alleviates cadmium toxicity by modulating ethylene production in Arabidopsis thaliana journal January 2020
PP2A and microtubules function in 5‐aminolevulinic acid‐mediated H 2 O 2 signaling in Arabidopsis guard cells journal June 2019
The PP2A-interactor TIP41 modulates ABA responses in Arabidopsis thaliana journal May 2018
Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase journal June 2016
The Dual Role of Nitric Oxide in Guard Cells: Promoting and Attenuating the ABA and Phospholipid-Derived Signals Leading to the Stomatal Closure journal April 2016
Abscisic Acid and Abiotic Stress Tolerance in Crop Plants journal May 2016
PP2A Phosphatase as a Regulator of ROS Signaling in Plants journal March 2016
Root Growth Adaptation is Mediated by PYLs ABA Receptor-PP2A Protein Phosphatase Complex text January 2020
Protein Phosphatase 2A in the Regulatory Network Underlying Biotic Stress Resistance in Plants journal June 2016
Silencing of the Wheat Protein Phosphatase 2A Catalytic Subunit TaPP2Ac Enhances Host Resistance to the Necrotrophic Pathogen Rhizoctonia cerealis journal October 2018

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